Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat

Background: Given the beneficial roles of adiponectin on body metabolism and its profound protective effects against metabolic disease, a better understanding of the adiponectin secretion and regulation is very important. Objectives: The objective of this study was to isolate, detect, quantify the...

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Main Authors:	Khattak, Muhammad Muzaffar Ali Khan, Abdul Rahman, Suzanah, Md Isa, Muhammad Lokman, Syed Osman Idid, Syed Zahir Idid, Md. Aris, Mohd. Aznan, Bakhtiar, Muhammad Taher, Phang, Ing Chia
Format:	Monograph
Language:	English
Published:	2017
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/57322/ http://irep.iium.edu.my/57322/1/END%20OF%20PROJECT%20REPORT%20FORM%20-%20MIRGS%20-%20Dr%20Muzaffar.pdf

id	iium-57322
recordtype	eprints
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Khattak, Muhammad Muzaffar Ali Khan Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Syed Osman Idid, Syed Zahir Idid Md. Aris, Mohd. Aznan Bakhtiar, Muhammad Taher Phang, Ing Chia Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
description	Background: Given the beneficial roles of adiponectin on body metabolism and its profound protective effects against metabolic disease, a better understanding of the adiponectin secretion and regulation is very important. Objectives: The objective of this study was to isolate, detect, quantify the total adiponectin in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb and tested on STZ-induced diabetic rats. Methods: Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Afterwards, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adiponectin using ELISA assay kit. Then continue with tested in STZ-induced diabetic rats on blood glucose and blood lipid, hormone measurement and lastly quantification of PPAR-α mRNA, AMPK mRNA, AdipoR1 mRNA, AdipoR2 mRNA and AdipoQ mRNA in the hepatocytes. The experiment was conducted in triplicates and the results are presented as means ± SD. The data was statistically analyzed by using SPSS (Version 21.0). One-way analysis of variance (ANOVA) was used and the data was considered statistically different at 95% confidence interval. Results: Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram, 0.15 gram and 0.15 gram respectively of protein amount which was 1 - 1.5 % from total tissue mass. The protein concentration in abdominal adipose tissue from chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml, respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml, respectively. Adiponectin concentration in beef abdominal adipose tissue significantly (P<0.05) higher compared to chicken and lamb. In animal study, the normal, insulin-treated, PCCA-treated (protein containing chicken adiponectin), PCBA-treated (protein containing beef adiponectin) and PCLA-treated (protein containing lamb adiponectin) diabetic groups exhibited significantly (P<0.05) reduced blood glucose levels after the treatment period when compared with the control diabetic (no treatment) group. Then, there is no significant difference of blood cholesterol levels in between groups, however the normal, insulin-treated, PCBA-treated and PCLA-treated diabetic groups shows a reduction of cholesterol level at the end of the experimental period. Similarly, the normal, insulin-treated, PCBA-treated and PCLA-treated diabetic groups exhibited significantly (P<0.05) reduced triglycerides levels after the treatment period when compared to the control diabetic (no treatment) group. Moreover, serum adiponectin concentration was significantly (P<0.05) higher in the normal, insulin-treated, PCBA-treated, PCCA-treated and PCLA-treated diabetic groups compared to control diabetic (no treatment) group. Nevertheless, serum insulin concentration was only significantly (P<0.05) higher in normal and insulin-treated groups as compared to control diabetic (no treatment) group. Lastly, AMPK, AdipoR1 and AdipoR2 mRNAs was significantly (p<0.05) increased in the normal, insulin-treated, PCBA-treated, PCCA-treated and PCLA-treated diabetic groups compared to control diabetic (no treatment) group. Then, PPAR-α mRNA was only significant (p<0.05) for normal, insulin and PCCA-treated diabetic groups compared to control diabetic (no treatment) group. However, there is no significant different between groups for AdipoQ mRNA. Conclusion: The present study suggests that beef protein had the highest amount of adiponectin protein and gave highest positive effects on STZ-induced diabetic rats. Thus, Adiponectin proteins extracted from wasted adipose tissues in meat sources can be one of the promising target for future novel pharmacological and therapeutic treatment/prevention for insulin resistance and metabolic diseases.
format	Monograph
author	Khattak, Muhammad Muzaffar Ali Khan Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Syed Osman Idid, Syed Zahir Idid Md. Aris, Mohd. Aznan Bakhtiar, Muhammad Taher Phang, Ing Chia
author_facet	Khattak, Muhammad Muzaffar Ali Khan Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Syed Osman Idid, Syed Zahir Idid Md. Aris, Mohd. Aznan Bakhtiar, Muhammad Taher Phang, Ing Chia
author_sort	Khattak, Muhammad Muzaffar Ali Khan
title	Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
title_short	Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
title_full	Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
title_fullStr	Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
title_full_unstemmed	Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat
title_sort	isolation, quantification and formulation of bioactive peptides (adipokines) for diabetes and obesity from adipose tissue in halal meat
publishDate	2017
url	http://irep.iium.edu.my/57322/ http://irep.iium.edu.my/57322/1/END%20OF%20PROJECT%20REPORT%20FORM%20-%20MIRGS%20-%20Dr%20Muzaffar.pdf
first_indexed	2023-09-18T21:21:01Z
last_indexed	2023-09-18T21:21:01Z
_version_	1777411881565159424
spelling	iium-573222018-03-29T07:13:43Z http://irep.iium.edu.my/57322/ Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat Khattak, Muhammad Muzaffar Ali Khan Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Syed Osman Idid, Syed Zahir Idid Md. Aris, Mohd. Aznan Bakhtiar, Muhammad Taher Phang, Ing Chia TX341 Nutrition. Foods and food supply Background: Given the beneficial roles of adiponectin on body metabolism and its profound protective effects against metabolic disease, a better understanding of the adiponectin secretion and regulation is very important. Objectives: The objective of this study was to isolate, detect, quantify the total adiponectin in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb and tested on STZ-induced diabetic rats. Methods: Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Afterwards, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adiponectin using ELISA assay kit. Then continue with tested in STZ-induced diabetic rats on blood glucose and blood lipid, hormone measurement and lastly quantification of PPAR-α mRNA, AMPK mRNA, AdipoR1 mRNA, AdipoR2 mRNA and AdipoQ mRNA in the hepatocytes. The experiment was conducted in triplicates and the results are presented as means ± SD. The data was statistically analyzed by using SPSS (Version 21.0). One-way analysis of variance (ANOVA) was used and the data was considered statistically different at 95% confidence interval. Results: Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram, 0.15 gram and 0.15 gram respectively of protein amount which was 1 - 1.5 % from total tissue mass. The protein concentration in abdominal adipose tissue from chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml, respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml, respectively. Adiponectin concentration in beef abdominal adipose tissue significantly (P<0.05) higher compared to chicken and lamb. In animal study, the normal, insulin-treated, PCCA-treated (protein containing chicken adiponectin), PCBA-treated (protein containing beef adiponectin) and PCLA-treated (protein containing lamb adiponectin) diabetic groups exhibited significantly (P<0.05) reduced blood glucose levels after the treatment period when compared with the control diabetic (no treatment) group. Then, there is no significant difference of blood cholesterol levels in between groups, however the normal, insulin-treated, PCBA-treated and PCLA-treated diabetic groups shows a reduction of cholesterol level at the end of the experimental period. Similarly, the normal, insulin-treated, PCBA-treated and PCLA-treated diabetic groups exhibited significantly (P<0.05) reduced triglycerides levels after the treatment period when compared to the control diabetic (no treatment) group. Moreover, serum adiponectin concentration was significantly (P<0.05) higher in the normal, insulin-treated, PCBA-treated, PCCA-treated and PCLA-treated diabetic groups compared to control diabetic (no treatment) group. Nevertheless, serum insulin concentration was only significantly (P<0.05) higher in normal and insulin-treated groups as compared to control diabetic (no treatment) group. Lastly, AMPK, AdipoR1 and AdipoR2 mRNAs was significantly (p<0.05) increased in the normal, insulin-treated, PCBA-treated, PCCA-treated and PCLA-treated diabetic groups compared to control diabetic (no treatment) group. Then, PPAR-α mRNA was only significant (p<0.05) for normal, insulin and PCCA-treated diabetic groups compared to control diabetic (no treatment) group. However, there is no significant different between groups for AdipoQ mRNA. Conclusion: The present study suggests that beef protein had the highest amount of adiponectin protein and gave highest positive effects on STZ-induced diabetic rats. Thus, Adiponectin proteins extracted from wasted adipose tissues in meat sources can be one of the promising target for future novel pharmacological and therapeutic treatment/prevention for insulin resistance and metabolic diseases. 2017 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/57322/1/END%20OF%20PROJECT%20REPORT%20FORM%20-%20MIRGS%20-%20Dr%20Muzaffar.pdf Khattak, Muhammad Muzaffar Ali Khan and Abdul Rahman, Suzanah and Md Isa, Muhammad Lokman and Syed Osman Idid, Syed Zahir Idid and Md. Aris, Mohd. Aznan and Bakhtiar, Muhammad Taher and Phang, Ing Chia (2017) Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat. Project Report. UNSPECIFIED. (Unpublished)

Isolation, quantification and formulation of bioactive peptides (Adipokines) for diabetes and obesity from adipose tissue in halal meat

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