Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract
Abstract: Background and Objective: The problems of bacterial diseases in aquaculture are primarily controlled by antibiotics. Medicinal plants and herbs which are seemed to be candidates of replacements for conventional antibiotics have therefore gained increasing interest. Current study was perfor...
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Asian Network for Scientific Information
2017
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iium-573152018-02-28T01:29:44Z http://irep.iium.edu.my/57315/ Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract A., Syahidah Saad, Che Roos Hassan, M. D. Rukayadi, Yaya Mohd Hassan, Norazian Mohd Salleh, Kamarudin RS Pharmacy and materia medica Abstract: Background and Objective: The problems of bacterial diseases in aquaculture are primarily controlled by antibiotics. Medicinal plants and herbs which are seemed to be candidates of replacements for conventional antibiotics have therefore gained increasing interest. Current study was performed to investigate the presence of phytochemical constituents, antibacterial activities and composition of antibacterial active compounds in methanolic extract of local herb, Piper betle . Methodology: Qualitative phytochemical analysis was firstly carried out to determine the possible active compounds in P. betle leaves methanolic extract. The antibacterial activities of major compounds from this extract against nine fish pathogenic bacteria were then assessed using TLC-bioautography agar overlay assay and their quantity were determined simultaneously by HPLC method. Results: The use of methanol has proved to be successful in extracting numerous bioactive compounds including antibacterial compounds. The TLC-bioautography assay revealed the inhibitory action of two compounds which were identified as hydroxychavicol and eugenol. The $-caryophyllene however was totally inactive against all the tested bacterial species. In this study, the concentration of hydroxychavicol in extract was found to be 374.72±2.79 mg g–1, while eugenol was 49.67±0.16 mg g–1. Conclusion: Based on these findings, it could be concluded that hydroxychavicol and eugenol were the responsible compounds for the promising antibacterial activity of P. betle leaves methanolic extract. This inhibitory action has significantly correlated with the amount of the compounds in extract. Due to its potential, the extract of P. betle leaves or it compounds can be alternative source of potent natural antibacterial agents for aquaculture disease management. Asian Network for Scientific Information 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57315/1/57315_Phytochemical%20analysis%2C%20identification.pdf application/pdf en http://irep.iium.edu.my/57315/2/57315_Phytochemical%20analysis%2C%20identification_SCOPUS.pdf A., Syahidah and Saad, Che Roos and Hassan, M. D. and Rukayadi, Yaya and Mohd Hassan, Norazian and Mohd Salleh, Kamarudin (2017) Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract. Pakistan Journal of Biological Sciences, 20 (2). pp. 70-81. ISSN 1028-8880 http://www.scialert.net/qredirect.php?doi=pjbs.2017.70.81&linkid=pdf 10.3923/pjbs.2017.70.81 |
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Online Access |
| language |
English English |
| topic |
RS Pharmacy and materia medica |
| spellingShingle |
RS Pharmacy and materia medica A., Syahidah Saad, Che Roos Hassan, M. D. Rukayadi, Yaya Mohd Hassan, Norazian Mohd Salleh, Kamarudin Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| description |
Abstract: Background and Objective: The problems of bacterial diseases in aquaculture are primarily controlled by antibiotics. Medicinal plants and herbs which are seemed to be candidates of replacements for conventional antibiotics have therefore gained increasing interest. Current study was performed to investigate the presence of phytochemical constituents, antibacterial activities and composition of antibacterial active compounds in methanolic extract of local herb, Piper betle . Methodology: Qualitative phytochemical analysis was firstly carried out to determine the possible active compounds in P. betle leaves methanolic extract. The antibacterial activities of major compounds from this extract against nine fish pathogenic bacteria were then assessed using TLC-bioautography agar overlay assay and their quantity were determined simultaneously by HPLC method. Results: The use of methanol has proved to be successful in extracting numerous bioactive compounds including antibacterial compounds. The TLC-bioautography assay revealed the inhibitory action of two compounds which were identified as hydroxychavicol and eugenol. The $-caryophyllene however was totally inactive against all the tested bacterial species. In this study, the concentration of hydroxychavicol in extract was found to be 374.72±2.79 mg g–1, while eugenol was 49.67±0.16 mg g–1. Conclusion: Based on these findings, it could be concluded that hydroxychavicol and eugenol were the responsible compounds for the promising antibacterial activity of P. betle leaves methanolic extract. This inhibitory action has significantly correlated with the amount of the compounds in extract. Due to its potential, the extract of P. betle leaves or it compounds can be alternative source of potent natural antibacterial agents for aquaculture disease management. |
| format |
Article |
| author |
A., Syahidah Saad, Che Roos Hassan, M. D. Rukayadi, Yaya Mohd Hassan, Norazian Mohd Salleh, Kamarudin |
| author_facet |
A., Syahidah Saad, Che Roos Hassan, M. D. Rukayadi, Yaya Mohd Hassan, Norazian Mohd Salleh, Kamarudin |
| author_sort |
A., Syahidah |
| title |
Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| title_short |
Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| title_full |
Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| title_fullStr |
Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| title_full_unstemmed |
Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| title_sort |
phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract |
| publisher |
Asian Network for Scientific Information |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/57315/ http://irep.iium.edu.my/57315/ http://irep.iium.edu.my/57315/ http://irep.iium.edu.my/57315/1/57315_Phytochemical%20analysis%2C%20identification.pdf http://irep.iium.edu.my/57315/2/57315_Phytochemical%20analysis%2C%20identification_SCOPUS.pdf |
| first_indexed |
2023-09-18T21:21:00Z |
| last_indexed |
2023-09-18T21:21:00Z |
| _version_ |
1777411880837447680 |