Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts

Background and Objective: Fibroblast stromal cells actively participates in tumor invasion by secreting matrix metalloproteinases (MMPs) within the tumor microenvironment. Expression of these enzymes is primarily regulated at a transcriptional level via interaction with transcription factors. Amon...

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Main Authors:	Ibrahim, Wisam Nabeel, Abdul Wahab, Ridhwan, Abdull Rasad, Mohammad Syaiful Bahari
Format:	Article
Language:	English English
Published:	Asian Network of Scienctific Information 2017
Subjects:	RB Pathology RL Dermatology
Online Access:	http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/2/17-25_3.pdf http://irep.iium.edu.my/57172/8/Expression%20of%20collagenases%20matrix%20metalloproteinases%20and%20YB-1%20oncogenic%20factor%20in%20malignant%20melanoma%20cancer%20cells%20and%20its%20regulation%20by%20stromal%20fibroblasts.pdf

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spelling	iium-571722018-04-25T05:44:54Z http://irep.iium.edu.my/57172/ Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari RB Pathology RL Dermatology Background and Objective: Fibroblast stromal cells actively participates in tumor invasion by secreting matrix metalloproteinases (MMPs) within the tumor microenvironment. Expression of these enzymes is primarily regulated at a transcriptional level via interaction with transcription factors. Among these factors, YB-1 oncogenic factor binds with different nucleic acids to exert its diverse influences. Also it represents an important prognostic indicator in many types of tumors. The aim of this study was to assess the expression of collagenases MMPs (MMP1, MMP8, MMP13) and the cellular proliferation in one of the most invasive types of cancer cell types which is the A375 malignant melanoma cancer cell line using co-culture settings. Also, the study attempted to assess the expression of YB-1 factor and its in vivo interaction with the AP-1 gene promoter sequence. Materials and Methods: The experiment involved growing A375 cells with CCD1079SK fibroblasts cells in co-culture environment. The proliferation of cells was determined using serial trypan blue assays, while the expression of YB-1, MMP1, MMP8 and MMP13 was determined by the use of real-time PCR and western blotting analysis. The potential interaction between YB-1 protein and AP-1 promoter sequence was assessed through chromatin immunoprecipitation (ChIP) assay. SPSS with independent t- test was used to compare cell proliferation and real-time PCR Ct mean values between samples. Results: In co-culture setting, the proliferation of A375 cancer cells was significantly faster than the cells in monoculture setting (5.1×105, 3×105 respectively in day 3) (p<0.05). Also, there was a significant increase in the expression of MMP1 enzyme. YB-1 and MMP8 were significantly expressed more in the A375 cancer cells in comparison with normal fibroblasts cells (p<0.05). Conclusion: The study confirms the role of stromal fibroblasts by enhancing the proliferation of melanoma cancer cells in vitro and increasing the expression of the MMP1 enzyme. In addition, YB-1 factor remains as an important prognostic indicator in cancer that might regulate expression of MMPs without binding to the AP-1 promoter sequence. Asian Network of Scienctific Information 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57172/2/17-25_3.pdf application/pdf en http://irep.iium.edu.my/57172/8/Expression%20of%20collagenases%20matrix%20metalloproteinases%20and%20YB-1%20oncogenic%20factor%20in%20malignant%20melanoma%20cancer%20cells%20and%20its%20regulation%20by%20stromal%20fibroblasts.pdf Ibrahim, Wisam Nabeel and Abdul Wahab, Ridhwan and Abdull Rasad, Mohammad Syaiful Bahari (2017) Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts. International Journal of Cancer Research, 13 (1). pp. 17-25. ISSN 1811-9727 E-ISSN 1811-9735 http://scialert.net/qredirect.php?doi=ijcr.2017.17.25&linkid=pdf 10.3923/ijcr.2017.17.25
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RB Pathology RL Dermatology
spellingShingle	RB Pathology RL Dermatology Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
description	Background and Objective: Fibroblast stromal cells actively participates in tumor invasion by secreting matrix metalloproteinases (MMPs) within the tumor microenvironment. Expression of these enzymes is primarily regulated at a transcriptional level via interaction with transcription factors. Among these factors, YB-1 oncogenic factor binds with different nucleic acids to exert its diverse influences. Also it represents an important prognostic indicator in many types of tumors. The aim of this study was to assess the expression of collagenases MMPs (MMP1, MMP8, MMP13) and the cellular proliferation in one of the most invasive types of cancer cell types which is the A375 malignant melanoma cancer cell line using co-culture settings. Also, the study attempted to assess the expression of YB-1 factor and its in vivo interaction with the AP-1 gene promoter sequence. Materials and Methods: The experiment involved growing A375 cells with CCD1079SK fibroblasts cells in co-culture environment. The proliferation of cells was determined using serial trypan blue assays, while the expression of YB-1, MMP1, MMP8 and MMP13 was determined by the use of real-time PCR and western blotting analysis. The potential interaction between YB-1 protein and AP-1 promoter sequence was assessed through chromatin immunoprecipitation (ChIP) assay. SPSS with independent t- test was used to compare cell proliferation and real-time PCR Ct mean values between samples. Results: In co-culture setting, the proliferation of A375 cancer cells was significantly faster than the cells in monoculture setting (5.1×105, 3×105 respectively in day 3) (p<0.05). Also, there was a significant increase in the expression of MMP1 enzyme. YB-1 and MMP8 were significantly expressed more in the A375 cancer cells in comparison with normal fibroblasts cells (p<0.05). Conclusion: The study confirms the role of stromal fibroblasts by enhancing the proliferation of melanoma cancer cells in vitro and increasing the expression of the MMP1 enzyme. In addition, YB-1 factor remains as an important prognostic indicator in cancer that might regulate expression of MMPs without binding to the AP-1 promoter sequence.
format	Article
author	Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari
author_facet	Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari
author_sort	Ibrahim, Wisam Nabeel
title	Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
title_short	Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
title_full	Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
title_fullStr	Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
title_full_unstemmed	Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
title_sort	expression of collagenases matrix metalloproteinases and yb-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts
publisher	Asian Network of Scienctific Information
publishDate	2017
url	http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/ http://irep.iium.edu.my/57172/2/17-25_3.pdf http://irep.iium.edu.my/57172/8/Expression%20of%20collagenases%20matrix%20metalloproteinases%20and%20YB-1%20oncogenic%20factor%20in%20malignant%20melanoma%20cancer%20cells%20and%20its%20regulation%20by%20stromal%20fibroblasts.pdf
first_indexed	2023-09-18T21:20:46Z
last_indexed	2023-09-18T21:20:46Z
_version_	1777411866045186048

Expression of collagenases matrix metalloproteinases and YB-1 oncogenic factor in malignant melanoma cancer cells and its regulation by stromal fibroblasts

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