Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia

The epigenetic changes of RELN that are involved in the development of dopaminergic neurons may fit the developmental theory of schizophrenia. However, evidence regarding the association of RELN DNA methylation with schizophrenia is far from sufficient, as studies have only been conducted on a few l...

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Main Authors:	Rahim Mohd, Nabil Fikri, A.Talib, Norlelawati, Abd. Rahim, Nour El Huda, Mohd Noor, Hanisah, Abdullah, Kartini, kuzaifah, Norsidah, Abdullah, Nor Zamzila
Format:	Article
Language:	English English English
Published:	Elsevier Ireland Ltd 2017
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/1/RELN%20Methylation%20in%20peripheral%20blood.pdf http://irep.iium.edu.my/56937/7/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_SCOPUS.pdf http://irep.iium.edu.my/56937/8/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_WoS.pdf

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spelling	iium-569372020-01-02T02:36:29Z http://irep.iium.edu.my/56937/ Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia Rahim Mohd, Nabil Fikri A.Talib, Norlelawati Abd. Rahim, Nour El Huda Mohd Noor, Hanisah Abdullah, Kartini kuzaifah, Norsidah Abdullah, Nor Zamzila R Medicine (General) The epigenetic changes of RELN that are involved in the development of dopaminergic neurons may fit the developmental theory of schizophrenia. However, evidence regarding the association of RELN DNA methylation with schizophrenia is far from sufficient, as studies have only been conducted on a few limited brain samples. As DNA methylation in the peripheral blood may mirror the changes taking place in the brain, the use of peripheral blood for a DNA methylation study in schizophrenia is feasible due to the scarcity of brain samples. Therefore, the aim of our study was to examine the relationship of DNA methylation levels of RELN promoters with schizophrenia using genomic DNA derived from the peripheral blood of patients with the disorder. The case control studies consisted of 110 schizophrenia participants and 122 healthy controls who had been recruited from the same district. After bisufhite conversion, the methylation levels of the DNA samples were calculated based on their differences of the Cq values assayed using the highly sensitive real-time MethyLight TaqMan® procedure. A significantly higher level of methylation of the RELN promoter was found in patients with schizophrenia compared to controls (p = 0.005) and also in males compared with females (p = 0.004). Subsequently, the RELN expression of the methylated group was 25 fold less than that of the non-methylated group. Based upon the assumption of parallel methylation changes in the brain and peripheral blood, we concluded that RELN DNA methylation might contribute to the pathogenesis of schizophrenia. However, the definite effects of methylation on RELN function during development and also in adult life still require further elaboration. Elsevier Ireland Ltd 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56937/1/RELN%20Methylation%20in%20peripheral%20blood.pdf application/pdf en http://irep.iium.edu.my/56937/7/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/56937/8/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_WoS.pdf Rahim Mohd, Nabil Fikri and A.Talib, Norlelawati and Abd. Rahim, Nour El Huda and Mohd Noor, Hanisah and Abdullah, Kartini and kuzaifah, Norsidah and Abdullah, Nor Zamzila (2017) Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia. Journal of Psychiatric Research, 88. pp. 28-37. ISSN 0022-3956 http://doi.org/10.1016/j.jpsychires.2016.12.020 10.1016/j.jpsychires.2016.12.020
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Rahim Mohd, Nabil Fikri A.Talib, Norlelawati Abd. Rahim, Nour El Huda Mohd Noor, Hanisah Abdullah, Kartini kuzaifah, Norsidah Abdullah, Nor Zamzila Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
description	The epigenetic changes of RELN that are involved in the development of dopaminergic neurons may fit the developmental theory of schizophrenia. However, evidence regarding the association of RELN DNA methylation with schizophrenia is far from sufficient, as studies have only been conducted on a few limited brain samples. As DNA methylation in the peripheral blood may mirror the changes taking place in the brain, the use of peripheral blood for a DNA methylation study in schizophrenia is feasible due to the scarcity of brain samples. Therefore, the aim of our study was to examine the relationship of DNA methylation levels of RELN promoters with schizophrenia using genomic DNA derived from the peripheral blood of patients with the disorder. The case control studies consisted of 110 schizophrenia participants and 122 healthy controls who had been recruited from the same district. After bisufhite conversion, the methylation levels of the DNA samples were calculated based on their differences of the Cq values assayed using the highly sensitive real-time MethyLight TaqMan® procedure. A significantly higher level of methylation of the RELN promoter was found in patients with schizophrenia compared to controls (p = 0.005) and also in males compared with females (p = 0.004). Subsequently, the RELN expression of the methylated group was 25 fold less than that of the non-methylated group. Based upon the assumption of parallel methylation changes in the brain and peripheral blood, we concluded that RELN DNA methylation might contribute to the pathogenesis of schizophrenia. However, the definite effects of methylation on RELN function during development and also in adult life still require further elaboration.
format	Article
author	Rahim Mohd, Nabil Fikri A.Talib, Norlelawati Abd. Rahim, Nour El Huda Mohd Noor, Hanisah Abdullah, Kartini kuzaifah, Norsidah Abdullah, Nor Zamzila
author_facet	Rahim Mohd, Nabil Fikri A.Talib, Norlelawati Abd. Rahim, Nour El Huda Mohd Noor, Hanisah Abdullah, Kartini kuzaifah, Norsidah Abdullah, Nor Zamzila
author_sort	Rahim Mohd, Nabil Fikri
title	Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
title_short	Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
title_full	Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
title_fullStr	Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
title_full_unstemmed	Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia
title_sort	reelin (reln) dna methylation in the peripheral blood of schizophrenia
publisher	Elsevier Ireland Ltd
publishDate	2017
url	http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/ http://irep.iium.edu.my/56937/1/RELN%20Methylation%20in%20peripheral%20blood.pdf http://irep.iium.edu.my/56937/7/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_SCOPUS.pdf http://irep.iium.edu.my/56937/8/56937_Reelin%20%28RELN%29%20DNA%20methylation%20in%20the%20peripheral%20blood%20of%20schizophrenia_WoS.pdf
first_indexed	2023-09-18T21:20:24Z
last_indexed	2023-09-18T21:20:24Z
_version_	1777411843701080064

Reelin (RELN) DNA methylation in the peripheral blood of schizophrenia

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