An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores

This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes suffi...

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Main Authors:	Park, J.H., Salmi, Mari L., Wan Salim, Wan Wardatul Amani, Rademacher, A.T., Wickizer, B., Schooley, Aaron, Benton, J.E., Cantero, A., Argote, P., Ren, M., Zhang, Min, Porterfield, David Marshall, Ricco, Antonio J., Roux, S. J., Rickus, Jenna L.
Format:	Article
Language:	English English English
Published:	Royal Society of Chemistry 2017
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/1/2017_SporeSat%20Ground-LoaC.pdf http://irep.iium.edu.my/56923/9/56923_An%20autonomous%20lab%20on%20a%20chip_SCOPUS.pdf http://irep.iium.edu.my/56923/15/56923_An%20autonomous%20lab%20on%20a%20chip%20for%20space_article.pdf

id	iium-56923
recordtype	eprints
spelling	iium-569232018-04-02T06:56:50Z http://irep.iium.edu.my/56923/ An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores Park, J.H. Salmi, Mari L. Wan Salim, Wan Wardatul Amani Rademacher, A.T. Wickizer, B. Schooley, Aaron Benton, J.E. Cantero, A. Argote, P. Ren, M. Zhang, Min Porterfield, David Marshall Ricco, Antonio J. Roux, S. J. Rickus, Jenna L. T Technology (General) This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions. Royal Society of Chemistry 2017-02-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56923/1/2017_SporeSat%20Ground-LoaC.pdf application/pdf en http://irep.iium.edu.my/56923/9/56923_An%20autonomous%20lab%20on%20a%20chip_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/56923/15/56923_An%20autonomous%20lab%20on%20a%20chip%20for%20space_article.pdf Park, J.H. and Salmi, Mari L. and Wan Salim, Wan Wardatul Amani and Rademacher, A.T. and Wickizer, B. and Schooley, Aaron and Benton, J.E. and Cantero, A. and Argote, P. and Ren, M. and Zhang, Min and Porterfield, David Marshall and Ricco, Antonio J. and Roux, S. J. and Rickus, Jenna L. (2017) An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores. Lab on a chip, 17 (6). pp. 1095-1103. ISSN 1473-0197 (In Press) https://www.ncbi.nlm.nih.gov/pubmed/28205656 10.1039/C6LC01370H
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	T Technology (General)
spellingShingle	T Technology (General) Park, J.H. Salmi, Mari L. Wan Salim, Wan Wardatul Amani Rademacher, A.T. Wickizer, B. Schooley, Aaron Benton, J.E. Cantero, A. Argote, P. Ren, M. Zhang, Min Porterfield, David Marshall Ricco, Antonio J. Roux, S. J. Rickus, Jenna L. An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
description	This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.
format	Article
author	Park, J.H. Salmi, Mari L. Wan Salim, Wan Wardatul Amani Rademacher, A.T. Wickizer, B. Schooley, Aaron Benton, J.E. Cantero, A. Argote, P. Ren, M. Zhang, Min Porterfield, David Marshall Ricco, Antonio J. Roux, S. J. Rickus, Jenna L.
author_facet	Park, J.H. Salmi, Mari L. Wan Salim, Wan Wardatul Amani Rademacher, A.T. Wickizer, B. Schooley, Aaron Benton, J.E. Cantero, A. Argote, P. Ren, M. Zhang, Min Porterfield, David Marshall Ricco, Antonio J. Roux, S. J. Rickus, Jenna L.
author_sort	Park, J.H.
title	An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
title_short	An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
title_full	An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
title_fullStr	An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
title_full_unstemmed	An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
title_sort	autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores
publisher	Royal Society of Chemistry
publishDate	2017
url	http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/ http://irep.iium.edu.my/56923/1/2017_SporeSat%20Ground-LoaC.pdf http://irep.iium.edu.my/56923/9/56923_An%20autonomous%20lab%20on%20a%20chip_SCOPUS.pdf http://irep.iium.edu.my/56923/15/56923_An%20autonomous%20lab%20on%20a%20chip%20for%20space_article.pdf
first_indexed	2023-09-18T21:20:23Z
last_indexed	2023-09-18T21:20:23Z
_version_	1777411842082078720

An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores

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