Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification

To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill eff...

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Main Authors: Rashid, Shah S, Alam, Md. Zahangir, Abdul Karim, Mohamed Ismail, Hamzah, Mohd. Salleh
Format: Conference or Workshop Item
Language:English
English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/5686/
http://irep.iium.edu.my/5686/
http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf
http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf
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recordtype eprints
spelling iium-56862012-01-19T06:49:48Z http://irep.iium.edu.my/5686/ Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification Rashid, Shah S, Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh TP Chemical technology TP248.13 Biotechnology To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain Trichoderma reesei RUT C-30. The study was conducted by using two different types of agents such as physical and chemical. Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for pretreatment of EFB to enhance the enzymatic saccharification of EFB. Among the physical pretreatment agents drying of EFB was found to be the best method and the maximum reducing sugar 99.06 mg/gm of EFB after 3 days (72 hours) was hydrolyzed from EFB which is 1.3 fold higher than the control. NaOH was proved to be the best among all the agents used in the pretreatment of EFB and 3% NaOH was far more effective on the enzymatic saccharification of EFB and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/gm of EFB) after 96 hours of incubation. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf application/pdf en http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf Rashid, Shah S, and Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh (2011) Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Rashid, Shah S,
Alam, Md. Zahangir
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
description To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain Trichoderma reesei RUT C-30. The study was conducted by using two different types of agents such as physical and chemical. Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for pretreatment of EFB to enhance the enzymatic saccharification of EFB. Among the physical pretreatment agents drying of EFB was found to be the best method and the maximum reducing sugar 99.06 mg/gm of EFB after 3 days (72 hours) was hydrolyzed from EFB which is 1.3 fold higher than the control. NaOH was proved to be the best among all the agents used in the pretreatment of EFB and 3% NaOH was far more effective on the enzymatic saccharification of EFB and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/gm of EFB) after 96 hours of incubation.
format Conference or Workshop Item
author Rashid, Shah S,
Alam, Md. Zahangir
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
author_facet Rashid, Shah S,
Alam, Md. Zahangir
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
author_sort Rashid, Shah S,
title Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
title_short Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
title_full Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
title_fullStr Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
title_full_unstemmed Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
title_sort pretreatment of empty fruit bunches (efb) for improved enzymatic saccharification
publishDate 2011
url http://irep.iium.edu.my/5686/
http://irep.iium.edu.my/5686/
http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf
http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf
first_indexed 2023-09-18T20:14:25Z
last_indexed 2023-09-18T20:14:25Z
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