Characterization of purified cellulase from fermentation of sewage sludge

The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in ce...

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Main Authors:	Mukhtaruddin, Muna Tasnim, Alam, Md. Zahangir, Hamzah, Mohd. Salleh
Format:	Conference or Workshop Item
Language:	English English
Published:	2011
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5685/ http://irep.iium.edu.my/5685/ http://irep.iium.edu.my/5685/1/ICBioE_2011_Mukhtaruddin_et_al.pdf http://irep.iium.edu.my/5685/4/characterization_of_purified_cellulase_from_fermentation_of_sewage_sludge.pdf

id	iium-5685
recordtype	eprints
spelling	iium-56852012-01-19T06:58:05Z http://irep.iium.edu.my/5685/ Characterization of purified cellulase from fermentation of sewage sludge Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Hamzah, Mohd. Salleh TP Chemical technology TP248.13 Biotechnology The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in certain industries as well as a key to solve the problem of environmental pollution and also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic materials. This study focuses on the characterization of cellulases produced from fermentation broth of sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out to characterize and checked the stability of the enzyme. The tests were pH stability, temperature stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48 hours turn to be the optimum incubation time. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5685/1/ICBioE_2011_Mukhtaruddin_et_al.pdf application/pdf en http://irep.iium.edu.my/5685/4/characterization_of_purified_cellulase_from_fermentation_of_sewage_sludge.pdf Mukhtaruddin, Muna Tasnim and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2011) Characterization of purified cellulase from fermentation of sewage sludge. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Hamzah, Mohd. Salleh Characterization of purified cellulase from fermentation of sewage sludge
description	The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in certain industries as well as a key to solve the problem of environmental pollution and also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic materials. This study focuses on the characterization of cellulases produced from fermentation broth of sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out to characterize and checked the stability of the enzyme. The tests were pH stability, temperature stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48 hours turn to be the optimum incubation time.
format	Conference or Workshop Item
author	Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_facet	Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_sort	Mukhtaruddin, Muna Tasnim
title	Characterization of purified cellulase from fermentation of sewage sludge
title_short	Characterization of purified cellulase from fermentation of sewage sludge
title_full	Characterization of purified cellulase from fermentation of sewage sludge
title_fullStr	Characterization of purified cellulase from fermentation of sewage sludge
title_full_unstemmed	Characterization of purified cellulase from fermentation of sewage sludge
title_sort	characterization of purified cellulase from fermentation of sewage sludge
publishDate	2011
url	http://irep.iium.edu.my/5685/ http://irep.iium.edu.my/5685/ http://irep.iium.edu.my/5685/1/ICBioE_2011_Mukhtaruddin_et_al.pdf http://irep.iium.edu.my/5685/4/characterization_of_purified_cellulase_from_fermentation_of_sewage_sludge.pdf
first_indexed	2023-09-18T20:14:25Z
last_indexed	2023-09-18T20:14:25Z
_version_	1777407691735433216

Characterization of purified cellulase from fermentation of sewage sludge

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