The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure

Objectives/Research Problem: Studies have reported that exposure to the alkylating drug, cyclophosphamide (CPA), for cancer treatment results in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sp...

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Main Authors:	Abdul Samat, Nadia Hanis, Abdul Rahman, Suzanah, Md Isa, Muhammad Lokman, Ahmad, Nur Amalina, Abdullah, 'Afif Raihan, Zahri, Syazana
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/56846/ http://irep.iium.edu.my/56846/1/KRW%202016_Nadia.pdf http://irep.iium.edu.my/56846/2/KAHS%20RW%202016_NADIA%20HANIS%20ORAL%20PPT.pdf

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recordtype	eprints
spelling	iium-568462017-05-09T07:22:32Z http://irep.iium.edu.my/56846/ The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Ahmad, Nur Amalina Abdullah, 'Afif Raihan Zahri, Syazana RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action Objectives/Research Problem: Studies have reported that exposure to the alkylating drug, cyclophosphamide (CPA), for cancer treatment results in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential of Nigella sativa extract (NSE) and thymoquinone (TQ) in the enhancement of sperm and embryo quality following exposure to the damaging effects of the anticancer drug. Materials and Method: Thirty male ICR mice were divided into six groups; Group 1: Vehicle-treated control (normal saline), Group 2: CPA-only, Group 3: TQ-only, Group 4: NSE-only, Group 5: CPA followed by TQ and Group 6: CPA followed by NSE. CPA is a one-time administration at a dose of 200 mg/kg via intraperitoneal injection. Both NSE and TQ were administered at 10 mg/kg on alternate days either on their own or in the combination with CPA. Animals were sacrificed at 33 days by cervical dislocation and sperm from caudal epididymis were taken for analysis, followed by in vitro fertilization (IVF) with the eggs from untreated female ICR mice. Fertilization rates and embryo development were assessed and monitored for 5 days. Results and Discussion: Results showed that NSE and TQ supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm. NSE and TQ supplementation have been shown to have significant effect (p<0.05) on the percentage of motile sperm as well as the number of abnormal sperm. Four types of abnormalities of the sperm were found which include folded sperm, amorphous, banana-like and the lack of usual hook. Embryo quality showed a significant improvement following supplementation of TQ and NSE (p<0.05). Conclusion: Overall, both NSE and TQ have indicated chemo-preventive potential against the cytotoxicity of CPA on reproductive capacity and fertility in mice. 2016-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56846/1/KRW%202016_Nadia.pdf application/pdf en http://irep.iium.edu.my/56846/2/KAHS%20RW%202016_NADIA%20HANIS%20ORAL%20PPT.pdf Abdul Samat, Nadia Hanis and Abdul Rahman, Suzanah and Md Isa, Muhammad Lokman and Ahmad, Nur Amalina and Abdullah, 'Afif Raihan and Zahri, Syazana (2016) The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21 - 25 November 2016, KAHS Examination Hall & Lecture Hall 1. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Ahmad, Nur Amalina Abdullah, 'Afif Raihan Zahri, Syazana The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
description	Objectives/Research Problem: Studies have reported that exposure to the alkylating drug, cyclophosphamide (CPA), for cancer treatment results in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential of Nigella sativa extract (NSE) and thymoquinone (TQ) in the enhancement of sperm and embryo quality following exposure to the damaging effects of the anticancer drug. Materials and Method: Thirty male ICR mice were divided into six groups; Group 1: Vehicle-treated control (normal saline), Group 2: CPA-only, Group 3: TQ-only, Group 4: NSE-only, Group 5: CPA followed by TQ and Group 6: CPA followed by NSE. CPA is a one-time administration at a dose of 200 mg/kg via intraperitoneal injection. Both NSE and TQ were administered at 10 mg/kg on alternate days either on their own or in the combination with CPA. Animals were sacrificed at 33 days by cervical dislocation and sperm from caudal epididymis were taken for analysis, followed by in vitro fertilization (IVF) with the eggs from untreated female ICR mice. Fertilization rates and embryo development were assessed and monitored for 5 days. Results and Discussion: Results showed that NSE and TQ supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm. NSE and TQ supplementation have been shown to have significant effect (p<0.05) on the percentage of motile sperm as well as the number of abnormal sperm. Four types of abnormalities of the sperm were found which include folded sperm, amorphous, banana-like and the lack of usual hook. Embryo quality showed a significant improvement following supplementation of TQ and NSE (p<0.05). Conclusion: Overall, both NSE and TQ have indicated chemo-preventive potential against the cytotoxicity of CPA on reproductive capacity and fertility in mice.
format	Conference or Workshop Item
author	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Ahmad, Nur Amalina Abdullah, 'Afif Raihan Zahri, Syazana
author_facet	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Ahmad, Nur Amalina Abdullah, 'Afif Raihan Zahri, Syazana
author_sort	Abdul Samat, Nadia Hanis
title	The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_short	The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_full	The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_fullStr	The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_full_unstemmed	The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_sort	potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
publishDate	2016
url	http://irep.iium.edu.my/56846/ http://irep.iium.edu.my/56846/1/KRW%202016_Nadia.pdf http://irep.iium.edu.my/56846/2/KAHS%20RW%202016_NADIA%20HANIS%20ORAL%20PPT.pdf
first_indexed	2023-09-18T21:20:15Z
last_indexed	2023-09-18T21:20:15Z
_version_	1777411834112901120

The potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure

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