Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells

Eurycoma longifolia, Nigella sativa and Hibiscus Sabdariffa are widely used as herbal remedies and natural supplements. It would be interesting to examine the effects of extracts from these natural products on significant cancers affecting the local health scene. Ovarian cancer is the most lethal of...

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Main Authors:	Abdullah, 'Afif Raihan, Abdul Rahman, Suzanah, Kaderi, Mohd Arifin, Chan, Kit-Lam, Abdul Samat, Nadia Hanis, Ahmad, Nur Amalina, Zahri, Syazana
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RG Gynecology and obstetrics RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/56845/ http://irep.iium.edu.my/56845/13/56845_KRW%202016_Raihan_complete.pdf

id	iium-56845
recordtype	eprints
spelling	iium-568452017-05-09T05:53:29Z http://irep.iium.edu.my/56845/ Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells Abdullah, 'Afif Raihan Abdul Rahman, Suzanah Kaderi, Mohd Arifin Chan, Kit-Lam Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Zahri, Syazana RG Gynecology and obstetrics RM Therapeutics. Pharmacology Eurycoma longifolia, Nigella sativa and Hibiscus Sabdariffa are widely used as herbal remedies and natural supplements. It would be interesting to examine the effects of extracts from these natural products on significant cancers affecting the local health scene. Ovarian cancer is the most lethal of all gynaecological malignancies and it is the top four cancers among women in Malaysia. The current study is designed to investigate the effects of Eurycoma longifolia, Nigella sativa and Hibiscus sabdariffa on ovarian cancer cell line (Caov-3) in comparison with cisplatin, the current drug used in treatment regimes. The appropriate half maximal inhibitory concentration (IC50) of each extracts will be selected through MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assays and the morphological changes in the nucleus indicating cellular apoptosis will be assessed by Hoechst 33258 staining methods. The evaluation of apoptosis with Annexin V-PE/7-AAD and their effects on cell cycle profile with Propidium Iodide (PI) will be performed via flow cytometry. Real-time PCR will be conducted to detect the gene expression of pro-apoptotic, Bcl-2 and anti-apoptotic, Bax. Apoptosis evaluation will be further confirmed by the detection of DNA fragmentation through TUNEL assay. This work is currently in progress to obtain the IC50 value. It is expected that Eurycoma longifolia, Nigella sativa and Hibiscus sabdariffa extracts have cytotoxic effects on ovarian cancer cell and could be new alternatives in treating ovarian cancer. 2016-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56845/13/56845_KRW%202016_Raihan_complete.pdf Abdullah, 'Afif Raihan and Abdul Rahman, Suzanah and Kaderi, Mohd Arifin and Chan, Kit-Lam and Abdul Samat, Nadia Hanis and Ahmad, Nur Amalina and Zahri, Syazana (2016) Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21 - 25 November 2016, KAHS Examination Hall & Lecture Hall 1. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RG Gynecology and obstetrics RM Therapeutics. Pharmacology
spellingShingle	RG Gynecology and obstetrics RM Therapeutics. Pharmacology Abdullah, 'Afif Raihan Abdul Rahman, Suzanah Kaderi, Mohd Arifin Chan, Kit-Lam Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Zahri, Syazana Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
description	Eurycoma longifolia, Nigella sativa and Hibiscus Sabdariffa are widely used as herbal remedies and natural supplements. It would be interesting to examine the effects of extracts from these natural products on significant cancers affecting the local health scene. Ovarian cancer is the most lethal of all gynaecological malignancies and it is the top four cancers among women in Malaysia. The current study is designed to investigate the effects of Eurycoma longifolia, Nigella sativa and Hibiscus sabdariffa on ovarian cancer cell line (Caov-3) in comparison with cisplatin, the current drug used in treatment regimes. The appropriate half maximal inhibitory concentration (IC50) of each extracts will be selected through MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assays and the morphological changes in the nucleus indicating cellular apoptosis will be assessed by Hoechst 33258 staining methods. The evaluation of apoptosis with Annexin V-PE/7-AAD and their effects on cell cycle profile with Propidium Iodide (PI) will be performed via flow cytometry. Real-time PCR will be conducted to detect the gene expression of pro-apoptotic, Bcl-2 and anti-apoptotic, Bax. Apoptosis evaluation will be further confirmed by the detection of DNA fragmentation through TUNEL assay. This work is currently in progress to obtain the IC50 value. It is expected that Eurycoma longifolia, Nigella sativa and Hibiscus sabdariffa extracts have cytotoxic effects on ovarian cancer cell and could be new alternatives in treating ovarian cancer.
format	Conference or Workshop Item
author	Abdullah, 'Afif Raihan Abdul Rahman, Suzanah Kaderi, Mohd Arifin Chan, Kit-Lam Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Zahri, Syazana
author_facet	Abdullah, 'Afif Raihan Abdul Rahman, Suzanah Kaderi, Mohd Arifin Chan, Kit-Lam Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Zahri, Syazana
author_sort	Abdullah, 'Afif Raihan
title	Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
title_short	Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
title_full	Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
title_fullStr	Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
title_full_unstemmed	Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells
title_sort	anticancer effects of eurycoma longifolia, nigella sativa and hibiscus sabdariffa on ovarian cancer cells
publishDate	2016
url	http://irep.iium.edu.my/56845/ http://irep.iium.edu.my/56845/13/56845_KRW%202016_Raihan_complete.pdf
first_indexed	2023-09-18T21:20:15Z
last_indexed	2023-09-18T21:20:15Z
_version_	1777411833966100480

Anticancer effects of eurycoma longifolia, Nigella sativa and hibiscus sabdariffa on ovarian cancer cells

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