Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment
Objectives/Research Problem: Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore, this work is performed to study the effects of standardized extract of Eurycoma longifol...
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2016
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| Online Access: | http://irep.iium.edu.my/56843/ http://irep.iium.edu.my/56843/12/56843_KRW%202016_Amalina_complete.pdf |
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iium-568432017-05-09T06:49:08Z http://irep.iium.edu.my/56843/ Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment Ahmad, Nur Amalina Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Zahri, Syazana Abdullah, 'Afif Raihan Chan, Kit-Lam RG Gynecology and obstetrics RM Therapeutics. Pharmacology Objectives/Research Problem: Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore, this work is performed to study the effects of standardized extract of Eurycoma longifolia, TAF 273, on oestrous cycle and some reproductive functionality in rats. Materials and Method: Female Sprague-Dawley rats (7-8 weeks old) weighing between 170-200 g were used in this study. Vaginal smear of each rat was monitored daily for 2-weeks period (cover 3 cycles) in order to select females with normal cycle (4-5 days). Those with regular cycle were randomized into four groups of 6 animals each. Rats in the treatment group received the extract orally at different doses (50, 75 and 100 mg/kg body weight) for 14-days, whereas the control group received an equal volume of distilled water in a similar manner. The oestrous cycle pattern of both control and treatment groups were monitored daily and recorded during the 14-days treatment. The body weight of the females was recorded before and after 14-days treatment. At the end of the experiments, blood samples were collected and the serum were analysed for testosterone, oestradiol, progesterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels. Ovaries were removed, weighed and examined for histomorphological changes. Results and Discussion: The results showed that the administration of TAF 273 has some changes on the analysed variables. Conclusion: The data obtained did not indicate that Eurycoma longifolia standardized extract exhibited any detrimental effects on the oestrous cycle, ovarian histomorphology and female reproductive hormones. 2016-11 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56843/12/56843_KRW%202016_Amalina_complete.pdf Ahmad, Nur Amalina and Abdul Rahman, Suzanah and Abdul Samat, Nadia Hanis and Zahri, Syazana and Abdullah, 'Afif Raihan and Chan, Kit-Lam (2016) Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21 - 25 November 2016, KAHS Examination Hall & ​Lecture Hall 1. (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
RG Gynecology and obstetrics RM Therapeutics. Pharmacology |
| spellingShingle |
RG Gynecology and obstetrics RM Therapeutics. Pharmacology Ahmad, Nur Amalina Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Zahri, Syazana Abdullah, 'Afif Raihan Chan, Kit-Lam Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| description |
Objectives/Research Problem:
Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore, this work is performed to study the effects of standardized extract of Eurycoma longifolia, TAF 273, on oestrous cycle and some reproductive functionality in rats.
Materials and Method:
Female Sprague-Dawley rats (7-8 weeks old) weighing between 170-200 g were used in this study. Vaginal smear of each rat was monitored daily for 2-weeks period (cover 3 cycles) in order to select females with normal cycle (4-5 days). Those with regular cycle were randomized into four groups of 6 animals each. Rats in the treatment group received the extract orally at different doses (50, 75 and 100 mg/kg body weight) for 14-days, whereas the control group received an equal volume of distilled water in a similar manner. The oestrous cycle pattern of both control and treatment groups were monitored daily and recorded during the 14-days treatment. The body weight of the females was recorded before and after 14-days treatment. At the end of the experiments, blood samples were collected and the serum were analysed for testosterone, oestradiol, progesterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels. Ovaries were removed, weighed and examined for histomorphological changes.
Results and Discussion:
The results showed that the administration of TAF 273 has some changes on the analysed variables.
Conclusion:
The data obtained did not indicate that Eurycoma longifolia standardized extract exhibited any detrimental effects on the oestrous cycle, ovarian histomorphology and female reproductive hormones. |
| format |
Conference or Workshop Item |
| author |
Ahmad, Nur Amalina Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Zahri, Syazana Abdullah, 'Afif Raihan Chan, Kit-Lam |
| author_facet |
Ahmad, Nur Amalina Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Zahri, Syazana Abdullah, 'Afif Raihan Chan, Kit-Lam |
| author_sort |
Ahmad, Nur Amalina |
| title |
Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| title_short |
Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| title_full |
Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| title_fullStr |
Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| title_full_unstemmed |
Effects of eurycoma longifolia (TAF 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| title_sort |
effects of eurycoma longifolia (taf 273) on oestrous cycle and reproductive hormones of normal rats during 14-days treatment |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/56843/ http://irep.iium.edu.my/56843/12/56843_KRW%202016_Amalina_complete.pdf |
| first_indexed |
2023-09-18T21:20:15Z |
| last_indexed |
2023-09-18T21:20:15Z |
| _version_ |
1777411833689276416 |