Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica

Introduction: Andrographis paniculata, Tinospora crispa and Centella asiatica are known to have various pharmacological functions. This research was carried out to investigate the antibacterial activities of protein extracts from A. paniculata, T. crispa and C. asiatica.Methods:Total soluble protein...

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Main Authors:	Md Zin, Noor Hasniza, Ahmad, Nurul Fatihah, Muhamad Bunnori, Noraslinda, Abdul Wahab, Widya, Haron, Normah
Format:	Conference or Workshop Item
Language:	English English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2016
Subjects:	QK Botany QR Microbiology
Online Access:	http://irep.iium.edu.my/56790/ http://irep.iium.edu.my/56790/ http://irep.iium.edu.my/56790/13/56790_poster.pdf http://irep.iium.edu.my/56790/14/56790_abstract.pdf

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recordtype	eprints
spelling	iium-567902017-05-17T04:02:05Z http://irep.iium.edu.my/56790/ Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica Md Zin, Noor Hasniza Ahmad, Nurul Fatihah Muhamad Bunnori, Noraslinda Abdul Wahab, Widya Haron, Normah QK Botany QR Microbiology Introduction: Andrographis paniculata, Tinospora crispa and Centella asiatica are known to have various pharmacological functions. This research was carried out to investigate the antibacterial activities of protein extracts from A. paniculata, T. crispa and C. asiatica.Methods:Total soluble proteins from these herbs were extracted using a modified TCA/acetone method.The protein extracts were then quantified using Bradford assay and separated using SDS-PAGE.The antibacterial activities were determined by disc diffusion method.Results and discussion:T. crispa had a significantly higher amount of proteins (83.86 ±0.4 µg/µl) compared to A.paniculata (81.57±0.4 µg/µl) and C. asiatica (78.93 ±0.5 µg/µl). The proteins separated by SDS-PAGE were ranged from 30kDa to 260kDa, 25kDa to 110kDa and 25kDa to 160kDa for A. paniculata, T. crispa and C. Asiatic, respectively. The high abundance proteins were observed in A. paniculata and T. crispa but not in C.asitica. Protein extracts from C. asiatica have demonstrated antibacterial activity against all tested bacteria with the diameter of inhibition zone of 11.0 ± 0.5 mm, 12.3 ± 0.6 mm, 10.7 ± 0.7 mm and 20.0 ± 0.8 mm against B. cereus, S.aureus, K. pneumonia and S. typhimurium respectively. Meanwhile, protein extracts of A. paniculata showed a positive antibacterial activity only against B.cereus (13.7 ± 0.4 mm), S. aureus (7.0 ± 0.8 mm) and S. typhimurium (11.5 ± 0.3 mm). Protein extracts from T. crispa only showed a positive antibacterial activity against B.cereus (9.7 ± 0.5 mm).Conclusion:There is a constant need in the discovery of new antibiotics for the treatment of infectious diseases. Kulliyyah of Medicine, International Islamic University Malaysia 2016-08-04 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56790/13/56790_poster.pdf application/pdf en http://irep.iium.edu.my/56790/14/56790_abstract.pdf Md Zin, Noor Hasniza and Ahmad, Nurul Fatihah and Muhamad Bunnori, Noraslinda and Abdul Wahab, Widya and Haron, Normah (2016) Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-64.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QK Botany QR Microbiology
spellingShingle	QK Botany QR Microbiology Md Zin, Noor Hasniza Ahmad, Nurul Fatihah Muhamad Bunnori, Noraslinda Abdul Wahab, Widya Haron, Normah Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
description	Introduction: Andrographis paniculata, Tinospora crispa and Centella asiatica are known to have various pharmacological functions. This research was carried out to investigate the antibacterial activities of protein extracts from A. paniculata, T. crispa and C. asiatica.Methods:Total soluble proteins from these herbs were extracted using a modified TCA/acetone method.The protein extracts were then quantified using Bradford assay and separated using SDS-PAGE.The antibacterial activities were determined by disc diffusion method.Results and discussion:T. crispa had a significantly higher amount of proteins (83.86 ±0.4 µg/µl) compared to A.paniculata (81.57±0.4 µg/µl) and C. asiatica (78.93 ±0.5 µg/µl). The proteins separated by SDS-PAGE were ranged from 30kDa to 260kDa, 25kDa to 110kDa and 25kDa to 160kDa for A. paniculata, T. crispa and C. Asiatic, respectively. The high abundance proteins were observed in A. paniculata and T. crispa but not in C.asitica. Protein extracts from C. asiatica have demonstrated antibacterial activity against all tested bacteria with the diameter of inhibition zone of 11.0 ± 0.5 mm, 12.3 ± 0.6 mm, 10.7 ± 0.7 mm and 20.0 ± 0.8 mm against B. cereus, S.aureus, K. pneumonia and S. typhimurium respectively. Meanwhile, protein extracts of A. paniculata showed a positive antibacterial activity only against B.cereus (13.7 ± 0.4 mm), S. aureus (7.0 ± 0.8 mm) and S. typhimurium (11.5 ± 0.3 mm). Protein extracts from T. crispa only showed a positive antibacterial activity against B.cereus (9.7 ± 0.5 mm).Conclusion:There is a constant need in the discovery of new antibiotics for the treatment of infectious diseases.
format	Conference or Workshop Item
author	Md Zin, Noor Hasniza Ahmad, Nurul Fatihah Muhamad Bunnori, Noraslinda Abdul Wahab, Widya Haron, Normah
author_facet	Md Zin, Noor Hasniza Ahmad, Nurul Fatihah Muhamad Bunnori, Noraslinda Abdul Wahab, Widya Haron, Normah
author_sort	Md Zin, Noor Hasniza
title	Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
title_short	Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
title_full	Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
title_fullStr	Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
title_full_unstemmed	Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
title_sort	antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/56790/ http://irep.iium.edu.my/56790/ http://irep.iium.edu.my/56790/13/56790_poster.pdf http://irep.iium.edu.my/56790/14/56790_abstract.pdf
first_indexed	2023-09-18T21:20:11Z
last_indexed	2023-09-18T21:20:11Z
_version_	1777411829329297408

Antibacterial activities of protein extracts from andrographis paniculata, tinospora crispa and centella asiatica

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