Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices
The macaque (Macaca fascicularis) monkeys are the third largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency and Ebola and Corona viruses, as well as being a religiously-protected and wildlife-protected species could not save the macaques from being...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English English |
| Published: |
Taylor and Francis Ltd.
2017
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/19/56745_development%20and%20validation.pdf http://irep.iium.edu.my/56745/2/56745_Development%20and%20validation_SCOPUS.pdf http://irep.iium.edu.my/56745/13/56745_Development%20and%20validation_WOS.pdf |
| id |
iium-56745 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-567452018-04-18T02:05:07Z http://irep.iium.edu.my/56745/ Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices Ali, Md Eaqub Abdul Rashid, Nur Raifana Abd Hamid, Sharifah Bee Hossain, Shouquat M.Azad ..,, Asing Hossain, Md Amzad Motalib Sarker, Md. Zaidul Islam QD Chemistry QP Physiology The macaque (Macaca fascicularis) monkeys are the third largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency and Ebola and Corona viruses, as well as being a religiously-protected and wildlife-protected species could not save the macaques from being over-hunted and consumed. Despite having needs, methods to detect monkey species in foods are rarely documented. To fill this gap, this article describes a monkey-specific polymerase chain reaction assay targeting a short-site (120 bp) of mitochondrial d-loop gene because the short-length targets are thermodynamically more stable than the longer ones under degrading states. Specificity was tested against 17 terrestrial and aquatic meat and fish species and no cross-species amplification was detected under raw, processed, and admixed states. The sensitivity of the assay was 0.0001 ng DNA under pure states and 0.1% monkey meats in binary meat mixtures. Finally, the assay was validated by digesting the polymerase chain reaction products with AluI and CViKI-1 and distinctive restriction fingerprints for macaque species were demonstrated. © 2017 Taylor & Francis Group, LLC. Taylor and Francis Ltd. 2017-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56745/19/56745_development%20and%20validation.pdf application/pdf en http://irep.iium.edu.my/56745/2/56745_Development%20and%20validation_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/56745/13/56745_Development%20and%20validation_WOS.pdf Ali, Md Eaqub and Abdul Rashid, Nur Raifana and Abd Hamid, Sharifah Bee and Hossain, Shouquat M.Azad and ..,, Asing and Hossain, Md Amzad Motalib and Sarker, Md. Zaidul Islam (2017) Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices. International Journal of Food Properties, 20 (1). pp. 231-245. ISSN 1094-2912 E-ISSN 1532-2386 http://www.tandfonline.com/doi/full/10.1080/10942912.2016.1154573 10.1080/10942912.2016.1154573 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English English |
| topic |
QD Chemistry QP Physiology |
| spellingShingle |
QD Chemistry QP Physiology Ali, Md Eaqub Abdul Rashid, Nur Raifana Abd Hamid, Sharifah Bee Hossain, Shouquat M.Azad ..,, Asing Hossain, Md Amzad Motalib Sarker, Md. Zaidul Islam Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| description |
The macaque (Macaca fascicularis) monkeys are the third largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency and Ebola and Corona viruses, as well as being a religiously-protected and wildlife-protected species could not save the macaques from being over-hunted and consumed. Despite having needs, methods to detect monkey species in foods are rarely documented. To fill this gap, this article describes a monkey-specific polymerase chain reaction assay targeting a short-site (120 bp) of mitochondrial d-loop gene because the short-length targets are thermodynamically more stable than the longer ones under degrading states. Specificity was tested against 17 terrestrial and aquatic meat and fish species and no cross-species amplification was detected under raw, processed, and admixed states. The sensitivity of the assay was 0.0001 ng DNA under pure states and 0.1% monkey meats in binary meat mixtures. Finally, the assay was validated by digesting the polymerase chain reaction products with AluI and CViKI-1 and distinctive restriction fingerprints for macaque species were demonstrated. © 2017 Taylor & Francis Group, LLC. |
| format |
Article |
| author |
Ali, Md Eaqub Abdul Rashid, Nur Raifana Abd Hamid, Sharifah Bee Hossain, Shouquat M.Azad ..,, Asing Hossain, Md Amzad Motalib Sarker, Md. Zaidul Islam |
| author_facet |
Ali, Md Eaqub Abdul Rashid, Nur Raifana Abd Hamid, Sharifah Bee Hossain, Shouquat M.Azad ..,, Asing Hossain, Md Amzad Motalib Sarker, Md. Zaidul Islam |
| author_sort |
Ali, Md Eaqub |
| title |
Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| title_short |
Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| title_full |
Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| title_fullStr |
Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| title_full_unstemmed |
Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices |
| title_sort |
development and validation of short-amplicon length pcr assay for macaques meat detection under complex matrices |
| publisher |
Taylor and Francis Ltd. |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/ http://irep.iium.edu.my/56745/19/56745_development%20and%20validation.pdf http://irep.iium.edu.my/56745/2/56745_Development%20and%20validation_SCOPUS.pdf http://irep.iium.edu.my/56745/13/56745_Development%20and%20validation_WOS.pdf |
| first_indexed |
2023-09-18T21:20:08Z |
| last_indexed |
2023-09-18T21:20:08Z |
| _version_ |
1777411825931911168 |