Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet

Previous studies have proved that chicken (Gallus gallus domesticus) feet can be one of the alternative sources of collagen. Besides of less risk of diseases and can be accepted by all religions, chicken feet also have high potential as a low cost of collagen source. Method of collagen extraction al...

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Main Authors:	Mokhtar, Nur Dhiya, Abdul Wahab, Widya, Hamdan, Nurul Ashima, Ab. Hadi, Hazrina, Abu Hassan, Muhamad Shafiq, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English English English
Published:	Emirates Research Publishing Limited, Dignified Researchers Publications 2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/56704/ http://irep.iium.edu.my/56704/1/56704_Extraction%20Optimization.pdf http://irep.iium.edu.my/56704/2/56704_presentation.pdf http://irep.iium.edu.my/56704/12/56704_Extraction%20Optimization%20and%20Characterization%20of%20Collagen.pdf

id	iium-56704
recordtype	eprints
spelling	iium-567042017-10-30T03:58:13Z http://irep.iium.edu.my/56704/ Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet Mokhtar, Nur Dhiya Abdul Wahab, Widya Hamdan, Nurul Ashima Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda Q Science (General) Previous studies have proved that chicken (Gallus gallus domesticus) feet can be one of the alternative sources of collagen. Besides of less risk of diseases and can be accepted by all religions, chicken feet also have high potential as a low cost of collagen source. Method of collagen extraction also plays an important role to obtain high percentage of end yield and the quality of collagen extracted. Optimization of collagen extraction was investigated by using three different soaking methods; 0.5 mol/L acetic acid with 0.1% bromelain, 0.5 mol/L acetic acid with 0.1% papain and 5% lactic acid for 36 hours. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction and potential of bromelain to improve collagen extraction. No significant different (P > 0.05) have been recorded between all three methods used in this research which indicated that all treatments were not affected the pH, swelling percentage, percentage of end yield and concentration of extracted collagen. However, lactic acid method was showed the highest percentage of end yield and concentration with reading of 30.04% and 11.66 mg/ml respectively. Type I collagen has been determined as major component of collagen from chicken feet based on the result of SDS-PAGE. It also proven that bromelain has same potential as papain in order to improve protein digestion Emirates Research Publishing Limited, Dignified Researchers Publications 2017-04-18 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56704/1/56704_Extraction%20Optimization.pdf application/pdf en http://irep.iium.edu.my/56704/2/56704_presentation.pdf application/pdf en http://irep.iium.edu.my/56704/12/56704_Extraction%20Optimization%20and%20Characterization%20of%20Collagen.pdf Mokhtar, Nur Dhiya and Abdul Wahab, Widya and Hamdan, Nurul Ashima and Ab. Hadi, Hazrina and Abu Hassan, Muhamad Shafiq and Muhamad Bunnori, Noraslinda (2017) Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet. In: 5th International Conference on Chemical, Agricultural, Biological and Environmental Sciences (CAFES-17), 18th-21st April 2017, Kyoto, Japan.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mokhtar, Nur Dhiya Abdul Wahab, Widya Hamdan, Nurul Ashima Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
description	Previous studies have proved that chicken (Gallus gallus domesticus) feet can be one of the alternative sources of collagen. Besides of less risk of diseases and can be accepted by all religions, chicken feet also have high potential as a low cost of collagen source. Method of collagen extraction also plays an important role to obtain high percentage of end yield and the quality of collagen extracted. Optimization of collagen extraction was investigated by using three different soaking methods; 0.5 mol/L acetic acid with 0.1% bromelain, 0.5 mol/L acetic acid with 0.1% papain and 5% lactic acid for 36 hours. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction and potential of bromelain to improve collagen extraction. No significant different (P > 0.05) have been recorded between all three methods used in this research which indicated that all treatments were not affected the pH, swelling percentage, percentage of end yield and concentration of extracted collagen. However, lactic acid method was showed the highest percentage of end yield and concentration with reading of 30.04% and 11.66 mg/ml respectively. Type I collagen has been determined as major component of collagen from chicken feet based on the result of SDS-PAGE. It also proven that bromelain has same potential as papain in order to improve protein digestion
format	Conference or Workshop Item
author	Mokhtar, Nur Dhiya Abdul Wahab, Widya Hamdan, Nurul Ashima Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda
author_facet	Mokhtar, Nur Dhiya Abdul Wahab, Widya Hamdan, Nurul Ashima Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda
author_sort	Mokhtar, Nur Dhiya
title	Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
title_short	Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
title_full	Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
title_fullStr	Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
title_full_unstemmed	Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
title_sort	extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet
publisher	Emirates Research Publishing Limited, Dignified Researchers Publications
publishDate	2017
url	http://irep.iium.edu.my/56704/ http://irep.iium.edu.my/56704/1/56704_Extraction%20Optimization.pdf http://irep.iium.edu.my/56704/2/56704_presentation.pdf http://irep.iium.edu.my/56704/12/56704_Extraction%20Optimization%20and%20Characterization%20of%20Collagen.pdf
first_indexed	2023-09-18T21:20:03Z
last_indexed	2023-09-18T21:20:03Z
_version_	1777411821091684352

Extraction optimization and characterization of collagen from chicken (gallus gallus domesticus) feet

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