The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells

Lee Indica and Stachytarpeta is the common plant in Malaysia and people are using as a traditional wound healing agent since an ancient era. The aim of this current study was to determine the diabetic wound healing activity. The NIH 3T3 mouse fibroblast cells and RAW 264.7 mouse macrophage cells c...

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Main Authors:	Wan Sulaiman, Wan Mohd Azizi, N.K., Sunzida, A. K., Azad
Format:	Article
Language:	English English
Published:	SILAE (Italo-Latin American Society of Ethnomedicine) 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/56591/ http://irep.iium.edu.my/56591/ http://irep.iium.edu.my/56591/1/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds_Scopus.pdf http://irep.iium.edu.my/56591/2/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds.pdf

id	iium-56591
recordtype	eprints
spelling	iium-565912017-04-19T07:18:37Z http://irep.iium.edu.my/56591/ The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells Wan Sulaiman, Wan Mohd Azizi N.K., Sunzida A. K., Azad RS Pharmacy and materia medica Lee Indica and Stachytarpeta is the common plant in Malaysia and people are using as a traditional wound healing agent since an ancient era. The aim of this current study was to determine the diabetic wound healing activity. The NIH 3T3 mouse fibroblast cells and RAW 264.7 mouse macrophage cells cultured to confluence in a six-well culture plate, and the culture medium drained away. The area of cell is scraped gently with a rubber stick to create a wound with 0.5mm width. The herbal extract added at 100 mg/mL to the culture while the control culture received only PBS. The migration of cells were observed after 24 h. The leea indica and stachytarpeta indica has shown a positive results when tested on RAW macrophage cells compared to its result when tested on NIH 3T3 mouse fibroblast cells which shows a less positive results. It can be concluded that both leea indica and stachytarpeta indica can be used to treat wound as it enhance the mitochondrial activities of cells. The extract from leea indica is more effective on tested cells compare than stachytarpeta indica. It is suitable to be used on macrophage cells rather than on fibroblast cells. It will support to the use of leea indica for the treatment of wound healing. SILAE (Italo-Latin American Society of Ethnomedicine) 2016-04-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56591/1/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds_Scopus.pdf application/pdf en http://irep.iium.edu.my/56591/2/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds.pdf Wan Sulaiman, Wan Mohd Azizi and N.K., Sunzida and A. K., Azad (2016) The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells. Pharmacologyonline, 2016 (1). pp. 139-145. ISSN 1827-8620 http://pharmacologyonline.silae.it/front/archives_2016_1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Wan Sulaiman, Wan Mohd Azizi N.K., Sunzida A. K., Azad The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
description	Lee Indica and Stachytarpeta is the common plant in Malaysia and people are using as a traditional wound healing agent since an ancient era. The aim of this current study was to determine the diabetic wound healing activity. The NIH 3T3 mouse fibroblast cells and RAW 264.7 mouse macrophage cells cultured to confluence in a six-well culture plate, and the culture medium drained away. The area of cell is scraped gently with a rubber stick to create a wound with 0.5mm width. The herbal extract added at 100 mg/mL to the culture while the control culture received only PBS. The migration of cells were observed after 24 h. The leea indica and stachytarpeta indica has shown a positive results when tested on RAW macrophage cells compared to its result when tested on NIH 3T3 mouse fibroblast cells which shows a less positive results. It can be concluded that both leea indica and stachytarpeta indica can be used to treat wound as it enhance the mitochondrial activities of cells. The extract from leea indica is more effective on tested cells compare than stachytarpeta indica. It is suitable to be used on macrophage cells rather than on fibroblast cells. It will support to the use of leea indica for the treatment of wound healing.
format	Article
author	Wan Sulaiman, Wan Mohd Azizi N.K., Sunzida A. K., Azad
author_facet	Wan Sulaiman, Wan Mohd Azizi N.K., Sunzida A. K., Azad
author_sort	Wan Sulaiman, Wan Mohd Azizi
title	The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
title_short	The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
title_full	The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
title_fullStr	The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
title_full_unstemmed	The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
title_sort	screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells
publisher	SILAE (Italo-Latin American Society of Ethnomedicine)
publishDate	2016
url	http://irep.iium.edu.my/56591/ http://irep.iium.edu.my/56591/ http://irep.iium.edu.my/56591/1/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds_Scopus.pdf http://irep.iium.edu.my/56591/2/56591_The%20screening%20of%20local%20herbs%20in%20treating%20non%20healing%20wounds.pdf
first_indexed	2023-09-18T21:19:51Z
last_indexed	2023-09-18T21:19:51Z
_version_	1777411808747847680

The screening of local herbs in treating non healing wounds and diabetic foot ulcers complications using nih 3t3 mouse fibroblast and raw 264.7 mouse macrophage cells

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