Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study

Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast...

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Main Authors:	Abdulrazzaq, Saad Mohammed, Amjad, Nasser Muhammad, Al-Kubaisi, Muna Khaleel Dheyab, Mizan, Shaikh
Format:	Article
Language:	English English English
Published:	Elsevier B.V. 2016
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf

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spelling	iium-565602018-10-18T02:07:53Z http://irep.iium.edu.my/56560/ Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh RC0254 Neoplasms. Tumors. Oncology (including Cancer) Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast tissues from 51 women with invasive ductal breast cancer. Leptin and leptin receptor in the tissues were estimated using immunohistochemistry (IHC). LEP and LEPR were localized at subcellular level by immunocytochemistry (ICC) using ultra-fine gold particle conjugated antibody, and visualized with transmission electron microscopy (TEM). IHC showed high presence of LEP and LEPR in 65% and 67% respectively of the breast cancer samples, 100% and 0% respectively of the adipose tissue samples, and no high presence in the non-cancer breast tissue samples. On TEM views both LEP and LEPR were found highly concentrated within the nucleus of the cancer cells, indicating that nucleus is the principal seat of action. However, presence of high concentration of LEP does not necessarily prove its over-expression, as often concluded, because LEP could be internalized from outside by LEPR in the cells. In contrast, LEPR is definitely over-expressed in the ductal breast cancer cells. Therefore, we hypothesize that over-expression of LEPR, rather than that of LEP has a fundamental role in breast carcinogenesis in particular, and probably for LEP-LEPR associated tumors in general. Elsevier B.V. 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf application/pdf en http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf application/pdf en http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf Abdulrazzaq, Saad Mohammed and Amjad, Nasser Muhammad and Al-Kubaisi, Muna Khaleel Dheyab and Mizan, Shaikh (2016) Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study. Biochemical and Biophysical Research Communications, 482 (4). pp. 1102-1106. ISSN 0006-291X E-ISSN 1090-2104 http://www.sciencedirect.com/science/article/pii/S0006291X16320393 http://doi.org/10.1016/j.bbrc.2016.11.165
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
description	Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast tissues from 51 women with invasive ductal breast cancer. Leptin and leptin receptor in the tissues were estimated using immunohistochemistry (IHC). LEP and LEPR were localized at subcellular level by immunocytochemistry (ICC) using ultra-fine gold particle conjugated antibody, and visualized with transmission electron microscopy (TEM). IHC showed high presence of LEP and LEPR in 65% and 67% respectively of the breast cancer samples, 100% and 0% respectively of the adipose tissue samples, and no high presence in the non-cancer breast tissue samples. On TEM views both LEP and LEPR were found highly concentrated within the nucleus of the cancer cells, indicating that nucleus is the principal seat of action. However, presence of high concentration of LEP does not necessarily prove its over-expression, as often concluded, because LEP could be internalized from outside by LEPR in the cells. In contrast, LEPR is definitely over-expressed in the ductal breast cancer cells. Therefore, we hypothesize that over-expression of LEPR, rather than that of LEP has a fundamental role in breast carcinogenesis in particular, and probably for LEP-LEPR associated tumors in general.
format	Article
author	Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh
author_facet	Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh
author_sort	Abdulrazzaq, Saad Mohammed
title	Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
title_short	Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
title_full	Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
title_fullStr	Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
title_full_unstemmed	Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
title_sort	subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
publisher	Elsevier B.V.
publishDate	2016
url	http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf
first_indexed	2023-09-18T21:19:49Z
last_indexed	2023-09-18T21:19:49Z
_version_	1777411805983801344

Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study

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