Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment

Direct sampling of microalgae from nature inevitably brings together the problem of massive growth of bacteria and fungi. In this study, bacterial and fungal contamination level was evaluated from the Chlorella sp. isolated from two different locations of local freshwater area. Attempts to obtain ax...

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Main Authors:	Mustapa, Mokhzanni, Sallehudin, Nor Jannah, Mohamed, Mohd Shamzi, Mohammad Noor, Normawaty, Ahmad Raus, Raha
Format:	Article
Language:	English English
Published:	Asian Research Publishing Network 2016
Subjects:	TD172 Environmental pollution
Online Access:	http://irep.iium.edu.my/56398/ http://irep.iium.edu.my/56398/ http://irep.iium.edu.my/56398/1/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment.pdf http://irep.iium.edu.my/56398/2/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment%20_Scopus.pdf

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spelling	iium-563982017-04-10T08:58:08Z http://irep.iium.edu.my/56398/ Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment Mustapa, Mokhzanni Sallehudin, Nor Jannah Mohamed, Mohd Shamzi Mohammad Noor, Normawaty Ahmad Raus, Raha TD172 Environmental pollution Direct sampling of microalgae from nature inevitably brings together the problem of massive growth of bacteria and fungi. In this study, bacterial and fungal contamination level was evaluated from the Chlorella sp. isolated from two different locations of local freshwater area. Attempts to obtain axenic Chlorella sp. culture by combinations of antibiotic and antifungal at different range of concentration treatment were investigated. It is evident that there were three different bacteria and two different fungi present in the culture, but apparently sterility can be achieved when ampicillin, cefotaxime and carbendazim cocktail were employed at concentrations of 700 µg/mL, 200 µg/mL and 0.1µg/mL, respectively. These concentrations are also proven harmless toward Chlorella sp. as higher concentrations inhibit the growth of microalgae. It was found that by streaking the contaminated microalgae twice onto the TAP agar containing the previously described cocktail, completely removed the contaminating fungi and bacteria from the culture. In conclusion, this study suggested that axenic Chlorella sp. can be attained with this method and cocktail recipe. Asian Research Publishing Network 2016-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56398/1/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment.pdf application/pdf en http://irep.iium.edu.my/56398/2/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment%20_Scopus.pdf Mustapa, Mokhzanni and Sallehudin, Nor Jannah and Mohamed, Mohd Shamzi and Mohammad Noor, Normawaty and Ahmad Raus, Raha (2016) Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment. ARPN Journal of Engineering and Applied Sciences, 11 (1). pp. 104-109. ISSN 1819-6608 http://www.arpnjournals.org/jeas/volume_01_2016.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TD172 Environmental pollution
spellingShingle	TD172 Environmental pollution Mustapa, Mokhzanni Sallehudin, Nor Jannah Mohamed, Mohd Shamzi Mohammad Noor, Normawaty Ahmad Raus, Raha Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
description	Direct sampling of microalgae from nature inevitably brings together the problem of massive growth of bacteria and fungi. In this study, bacterial and fungal contamination level was evaluated from the Chlorella sp. isolated from two different locations of local freshwater area. Attempts to obtain axenic Chlorella sp. culture by combinations of antibiotic and antifungal at different range of concentration treatment were investigated. It is evident that there were three different bacteria and two different fungi present in the culture, but apparently sterility can be achieved when ampicillin, cefotaxime and carbendazim cocktail were employed at concentrations of 700 µg/mL, 200 µg/mL and 0.1µg/mL, respectively. These concentrations are also proven harmless toward Chlorella sp. as higher concentrations inhibit the growth of microalgae. It was found that by streaking the contaminated microalgae twice onto the TAP agar containing the previously described cocktail, completely removed the contaminating fungi and bacteria from the culture. In conclusion, this study suggested that axenic Chlorella sp. can be attained with this method and cocktail recipe.
format	Article
author	Mustapa, Mokhzanni Sallehudin, Nor Jannah Mohamed, Mohd Shamzi Mohammad Noor, Normawaty Ahmad Raus, Raha
author_facet	Mustapa, Mokhzanni Sallehudin, Nor Jannah Mohamed, Mohd Shamzi Mohammad Noor, Normawaty Ahmad Raus, Raha
author_sort	Mustapa, Mokhzanni
title	Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
title_short	Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
title_full	Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
title_fullStr	Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
title_full_unstemmed	Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment
title_sort	decontamination of chlorella sp. culture using antibiotics and antifungal cocktail treatment
publisher	Asian Research Publishing Network
publishDate	2016
url	http://irep.iium.edu.my/56398/ http://irep.iium.edu.my/56398/ http://irep.iium.edu.my/56398/1/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment.pdf http://irep.iium.edu.my/56398/2/56398_Decontamination%20of%20Chlorella%20sp.%20Culture%20using%20antibiotics%20and%20antifungal%20cocktail%20treatment%20_Scopus.pdf
first_indexed	2023-09-18T21:19:33Z
last_indexed	2023-09-18T21:19:33Z
_version_	1777411789224411136

Decontamination of Chlorella sp. Culture using antibiotics and antifungal cocktail treatment

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