Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells

Tinospora crispa is a climber plant belonging to the family of Menispermaceae. This plant has been used traditionally in Indonesia to treat some diseases and conditions such as fever, malaria, rheumatism, diabetes, cholera and inflammation. The aims of this study was to investigate cytotoxic activit...

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Main Authors:	Adnan, Adek Zamrud, Bakhtiar, Muhammad Taher, Afriani, Tika, Roesma, Dewi Imelda, Putra, Andani Eka
Format:	Article
Language:	English English
Published:	Scholars Research Library 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/56396/ http://irep.iium.edu.my/56396/ http://irep.iium.edu.my/56396/1/56396_Cytotoxic%20activity%20assay.pdf http://irep.iium.edu.my/56396/2/56396_Cytotoxic%20activity%20assay_SCOPUS.pdf

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spelling	iium-563962017-04-10T06:33:31Z http://irep.iium.edu.my/56396/ Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka RS Pharmacy and materia medica Tinospora crispa is a climber plant belonging to the family of Menispermaceae. This plant has been used traditionally in Indonesia to treat some diseases and conditions such as fever, malaria, rheumatism, diabetes, cholera and inflammation. The aims of this study was to investigate cytotoxic activity of tinocrisposide extracted from the plant against human cancer cell lines. Cytotoxic activity of tinocrisposide in interval dose of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been investigated on H1299 and MCF-7 cell lines and their effects were quantified spectrophotometrically with the 3-(4,5 dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay at wavelength, λ 570 nm. Tinocrisposide in concentrations of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been treated against H1299 cell line and showed a percentage viability of 55.5; 67.70; 80.84; 81.09; 82.69; and 87.06% respectively, with IC50 70.9 μg/mL, whereas on MCF-7 cell line showed percentage viability of 80.71; 89.30; 92.76; 96.01; 96.58, and 102.66% respectively, with IC50 > 100 μg/mL. It can be concluded that tinocrisposide has not shown any cytotoxic activity against H1299 and MCF-7 cell line, but it can be used as a chemopreventive agent toward those cell lines. Scholars Research Library 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56396/1/56396_Cytotoxic%20activity%20assay.pdf application/pdf en http://irep.iium.edu.my/56396/2/56396_Cytotoxic%20activity%20assay_SCOPUS.pdf Adnan, Adek Zamrud and Bakhtiar, Muhammad Taher and Afriani, Tika and Roesma, Dewi Imelda and Putra, Andani Eka (2016) Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells. Der Pharmacia Lettre, 8 (18). pp. 102-106. ISSN 0975-5071 http://www.scholarsresearchlibrary.com/archive/dpl-volume-8-issue-16-year-2016.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
description	Tinospora crispa is a climber plant belonging to the family of Menispermaceae. This plant has been used traditionally in Indonesia to treat some diseases and conditions such as fever, malaria, rheumatism, diabetes, cholera and inflammation. The aims of this study was to investigate cytotoxic activity of tinocrisposide extracted from the plant against human cancer cell lines. Cytotoxic activity of tinocrisposide in interval dose of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been investigated on H1299 and MCF-7 cell lines and their effects were quantified spectrophotometrically with the 3-(4,5 dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay at wavelength, λ 570 nm. Tinocrisposide in concentrations of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been treated against H1299 cell line and showed a percentage viability of 55.5; 67.70; 80.84; 81.09; 82.69; and 87.06% respectively, with IC50 70.9 μg/mL, whereas on MCF-7 cell line showed percentage viability of 80.71; 89.30; 92.76; 96.01; 96.58, and 102.66% respectively, with IC50 > 100 μg/mL. It can be concluded that tinocrisposide has not shown any cytotoxic activity against H1299 and MCF-7 cell line, but it can be used as a chemopreventive agent toward those cell lines.
format	Article
author	Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka
author_facet	Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka
author_sort	Adnan, Adek Zamrud
title	Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
title_short	Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
title_full	Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
title_fullStr	Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
title_full_unstemmed	Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
title_sort	cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells
publisher	Scholars Research Library
publishDate	2016
url	http://irep.iium.edu.my/56396/ http://irep.iium.edu.my/56396/ http://irep.iium.edu.my/56396/1/56396_Cytotoxic%20activity%20assay.pdf http://irep.iium.edu.my/56396/2/56396_Cytotoxic%20activity%20assay_SCOPUS.pdf
first_indexed	2023-09-18T21:19:32Z
last_indexed	2023-09-18T21:19:32Z
_version_	1777411788926615552

Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells

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