Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)

Inhibitory effects of hydrogen sulfide could either be due to toxicity to living tissue or precipitation of sulfide salts which inhibit its utilization for cell growth. Growth, is undoubtedly affected by certain conditions, among which are nutrient types and availability, as well as the operational...

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Main Authors:	Ahmad, M.M., Azoddien, A.M., Zahari, M. A.K.M, Seman, M. N, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. 2017
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/1/Ahmad%20et%20al%202017-jceib.pdf

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spelling	iium-562912017-07-11T02:40:27Z http://irep.iium.edu.my/56291/ Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579) Ahmad, M.M. Azoddien, A.M. Zahari, M. A.K.M Seman, M. N Jami, Mohammed Saedi TP155 Chemical engineering TP248.13 Biotechnology Inhibitory effects of hydrogen sulfide could either be due to toxicity to living tissue or precipitation of sulfide salts which inhibit its utilization for cell growth. Growth, is undoubtedly affected by certain conditions, among which are nutrient types and availability, as well as the operational parameters of the fermenting medium. In this study, Bacillus cereus (ATCC 14579) was tested for potential sulfide biodegradation in a suspended growth medium of orbital shake flasks using a single milieu composition under defined operational parameters. Growth and sulfide oxidation efficiency were measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200 ppm S2- L-1 d1, 300 ppm S2- L-1 d-1 and 500 ppm S2- L-1 d-1 with corresponding appreciable cell growth measured at OD600 nm. Results indicated that it was possible to realise sulfide removal efficiency of 95% to 99% using this strain in an orbital shake flask within 24 hrs, as well as 65% to 78% within the first 6 hrs of inoculation. Overall, sulfide was reduced by 95% in 200 ppm and 300 ppm, while 99% in 500 ppm, respectively. While, the corresponding exponential cell growth recorded was 3.91, 3.80 and 3.61 in 200 ppm, 300 ppm and 500 ppm, respectively. This also translate to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L and 0.50 g/L in 200 ppm, 500 ppm and 300 ppm. Based on this finding, it was clear that this inoculum can utilize different sulfide concentration for growth and biosynthesis; thus can be employed to treat sulfide contaminated wastewater in a suspended growth form under simple nutrient composition and operational conditions. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. 2017-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56291/1/Ahmad%20et%20al%202017-jceib.pdf Ahmad, M.M. and Azoddien, A.M. and Zahari, M. A.K.M and Seman, M. N and Jami, Mohammed Saedi (2017) Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579). Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 1. pp. 83-96. E-ISSN Not available (Open access) http://jceib.ump.edu.my/images/JCEIB_VOLUME1/JCEIB-V1-04.pdf 10.15282/JCEIB-V1-04.31/3/2017/1.1.1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Ahmad, M.M. Azoddien, A.M. Zahari, M. A.K.M Seman, M. N Jami, Mohammed Saedi Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
description	Inhibitory effects of hydrogen sulfide could either be due to toxicity to living tissue or precipitation of sulfide salts which inhibit its utilization for cell growth. Growth, is undoubtedly affected by certain conditions, among which are nutrient types and availability, as well as the operational parameters of the fermenting medium. In this study, Bacillus cereus (ATCC 14579) was tested for potential sulfide biodegradation in a suspended growth medium of orbital shake flasks using a single milieu composition under defined operational parameters. Growth and sulfide oxidation efficiency were measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200 ppm S2- L-1 d1, 300 ppm S2- L-1 d-1 and 500 ppm S2- L-1 d-1 with corresponding appreciable cell growth measured at OD600 nm. Results indicated that it was possible to realise sulfide removal efficiency of 95% to 99% using this strain in an orbital shake flask within 24 hrs, as well as 65% to 78% within the first 6 hrs of inoculation. Overall, sulfide was reduced by 95% in 200 ppm and 300 ppm, while 99% in 500 ppm, respectively. While, the corresponding exponential cell growth recorded was 3.91, 3.80 and 3.61 in 200 ppm, 300 ppm and 500 ppm, respectively. This also translate to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L and 0.50 g/L in 200 ppm, 500 ppm and 300 ppm. Based on this finding, it was clear that this inoculum can utilize different sulfide concentration for growth and biosynthesis; thus can be employed to treat sulfide contaminated wastewater in a suspended growth form under simple nutrient composition and operational conditions.
format	Article
author	Ahmad, M.M. Azoddien, A.M. Zahari, M. A.K.M Seman, M. N Jami, Mohammed Saedi
author_facet	Ahmad, M.M. Azoddien, A.M. Zahari, M. A.K.M Seman, M. N Jami, Mohammed Saedi
author_sort	Ahmad, M.M.
title	Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
title_short	Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
title_full	Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
title_fullStr	Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
title_full_unstemmed	Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)
title_sort	biological sulfide oxidation and its impact on cell biomass synthesis by mesophilic bacterium bacillus cerues (atcc 14579)
publisher	Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.
publishDate	2017
url	http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/ http://irep.iium.edu.my/56291/1/Ahmad%20et%20al%202017-jceib.pdf
first_indexed	2023-09-18T21:19:23Z
last_indexed	2023-09-18T21:19:23Z
_version_	1777411779388768256

Biological sulfide oxidation and its impact on cell biomass synthesis by Mesophilic bacterium bacillus cerues (ATCC 14579)

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