A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms

Cardiovascular effects (e.g., tachycardia, hypo- and/or hypertension) are often clinical outcomes of snake envenoming. Malayan krait (Bungarus candidus) envenoming has been reported to cause cardiovascular effects that may be related to abnormalities in parasympathetic activity. However, the exact m...

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Main Authors:	Chaisakul, Janeyuth, Ahmad Rusmili, Muhamad Rusdi, Hodgson, Wayne C., Hatthachote, Panadda, Suwan, Kijja, Inchan, Anjaree, Chanhome, Lawan, Othman, Iekhsan, Chootip, Krongkarn
Format:	Article
Language:	English English
Published:	MDPI 2017
Subjects:	QL Zoology RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/1/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_article.pdf http://irep.iium.edu.my/56289/2/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_scopus.pdf

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spelling	iium-562892018-03-14T11:00:45Z http://irep.iium.edu.my/56289/ A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms Chaisakul, Janeyuth Ahmad Rusmili, Muhamad Rusdi Hodgson, Wayne C. Hatthachote, Panadda Suwan, Kijja Inchan, Anjaree Chanhome, Lawan Othman, Iekhsan Chootip, Krongkarn QL Zoology RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica Cardiovascular effects (e.g., tachycardia, hypo- and/or hypertension) are often clinical outcomes of snake envenoming. Malayan krait (Bungarus candidus) envenoming has been reported to cause cardiovascular effects that may be related to abnormalities in parasympathetic activity. However, the exact mechanism for this effect has yet to be determined. In the present study, we investigated the in vivo and in vitro cardiovascular effects of B. candidus venoms from Southern (BC-S) and Northeastern (BC-NE) Thailand. SDS-PAGE analysis of venoms showed some differences in the protein profile of the venoms. B. candidus venoms (50 µg/kg–100 µg/kg, i.v.) caused dose-dependent hypotension in anaesthetised rats. The highest dose caused sudden hypotension (phase I) followed by a return of mean arterial pressure to baseline levels and a decrease in heart rate with transient hypertension (phase II) prior to a small decrease in blood pressure (phase III). Prior administration of monovalent antivenom significantly attenuated the hypotension induced by venoms (100 µg/kg, i.v.). The sudden hypotensive effect of BC-NE venom was abolished by prior administration of hexamethonium (10 mg/kg, i.v.) or atropine (5 mg/kg, i.v.). BC-S and BC-NE venoms (0.1 µg/kg–100 µg/ml) induced concentration-dependent relaxation (EC50 = 8 ± 1 and 13 ± 3 µg/mL, respectively) in endothelium-intact aorta. The concentration–response curves were markedly shifted to the right by pre-incubation with L-NAME (0.2 mM), or removal of the endothelium, suggesting that endothelium-derived nitric oxide (NO) is likely to be responsible for venom-induced aortic relaxation. Our data indicate that the cardiovascular effects caused by B. candidus venoms may be due to a combination of vascular mediators (i.e., NO) and autonomic adaptation via nicotinic and muscarinic acetylcholine receptors MDPI 2017-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56289/1/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_article.pdf application/pdf en http://irep.iium.edu.my/56289/2/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_scopus.pdf Chaisakul, Janeyuth and Ahmad Rusmili, Muhamad Rusdi and Hodgson, Wayne C. and Hatthachote, Panadda and Suwan, Kijja and Inchan, Anjaree and Chanhome, Lawan and Othman, Iekhsan and Chootip, Krongkarn (2017) A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms. Toxins, 9 (4). pp. 1-11. ISSN 2072-6651 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408196/pdf/toxins-09-00122.pdf 10.3390/toxins9040122
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QL Zoology RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
spellingShingle	QL Zoology RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica Chaisakul, Janeyuth Ahmad Rusmili, Muhamad Rusdi Hodgson, Wayne C. Hatthachote, Panadda Suwan, Kijja Inchan, Anjaree Chanhome, Lawan Othman, Iekhsan Chootip, Krongkarn A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
description	Cardiovascular effects (e.g., tachycardia, hypo- and/or hypertension) are often clinical outcomes of snake envenoming. Malayan krait (Bungarus candidus) envenoming has been reported to cause cardiovascular effects that may be related to abnormalities in parasympathetic activity. However, the exact mechanism for this effect has yet to be determined. In the present study, we investigated the in vivo and in vitro cardiovascular effects of B. candidus venoms from Southern (BC-S) and Northeastern (BC-NE) Thailand. SDS-PAGE analysis of venoms showed some differences in the protein profile of the venoms. B. candidus venoms (50 µg/kg–100 µg/kg, i.v.) caused dose-dependent hypotension in anaesthetised rats. The highest dose caused sudden hypotension (phase I) followed by a return of mean arterial pressure to baseline levels and a decrease in heart rate with transient hypertension (phase II) prior to a small decrease in blood pressure (phase III). Prior administration of monovalent antivenom significantly attenuated the hypotension induced by venoms (100 µg/kg, i.v.). The sudden hypotensive effect of BC-NE venom was abolished by prior administration of hexamethonium (10 mg/kg, i.v.) or atropine (5 mg/kg, i.v.). BC-S and BC-NE venoms (0.1 µg/kg–100 µg/ml) induced concentration-dependent relaxation (EC50 = 8 ± 1 and 13 ± 3 µg/mL, respectively) in endothelium-intact aorta. The concentration–response curves were markedly shifted to the right by pre-incubation with L-NAME (0.2 mM), or removal of the endothelium, suggesting that endothelium-derived nitric oxide (NO) is likely to be responsible for venom-induced aortic relaxation. Our data indicate that the cardiovascular effects caused by B. candidus venoms may be due to a combination of vascular mediators (i.e., NO) and autonomic adaptation via nicotinic and muscarinic acetylcholine receptors
format	Article
author	Chaisakul, Janeyuth Ahmad Rusmili, Muhamad Rusdi Hodgson, Wayne C. Hatthachote, Panadda Suwan, Kijja Inchan, Anjaree Chanhome, Lawan Othman, Iekhsan Chootip, Krongkarn
author_facet	Chaisakul, Janeyuth Ahmad Rusmili, Muhamad Rusdi Hodgson, Wayne C. Hatthachote, Panadda Suwan, Kijja Inchan, Anjaree Chanhome, Lawan Othman, Iekhsan Chootip, Krongkarn
author_sort	Chaisakul, Janeyuth
title	A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
title_short	A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
title_full	A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
title_fullStr	A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
title_full_unstemmed	A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms
title_sort	pharmacological examination of the cardiovascular effects of malayan krait (bungarus candidus) venoms
publisher	MDPI
publishDate	2017
url	http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/ http://irep.iium.edu.my/56289/1/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_article.pdf http://irep.iium.edu.my/56289/2/56289_A%20pharmacological%20examination%20of%20the%20cardiovascular%20effects%20_scopus.pdf
first_indexed	2023-09-18T21:19:23Z
last_indexed	2023-09-18T21:19:23Z
_version_	1777411779058466816

A pharmacological examination of the cardiovascular effects of Malayan Krait (Bungarus candidus) venoms

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