Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth

The inhibitory effect of hydrogen sulfide has been indicated to activate a sequence of response mechanism through microbial mutual interactions. Bacterial mixed-culture (BMC) consortium has been found to reduce the complexity of oxidative stress through mutual interactions of helper bacteria thereby...

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Main Authors:	Ahmad, Mani Malam, Mohd Azoddein, Abd. Aziz, Mohd Zahari, Mior Ahmad Khusairi, Abu Seman, Mazrul Nizam, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	Penerbit Akademia Baru 2017
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/56288/ http://irep.iium.edu.my/56288/ http://irep.iium.edu.my/56288/1/Ahmad%20et%20al%202017.pdf

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spelling	iium-562882018-01-05T09:42:01Z http://irep.iium.edu.my/56288/ Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi TP155 Chemical engineering TP248.13 Biotechnology The inhibitory effect of hydrogen sulfide has been indicated to activate a sequence of response mechanism through microbial mutual interactions. Bacterial mixed-culture (BMC) consortium has been found to reduce the complexity of oxidative stress through mutual interactions of helper bacteria thereby facilitating the growth of the disadvantaged inoculum. The potential sulfide bioxidation of strain one (S1) and strain two (S2) mixed-culture bacterial consortiums were tested at different sulfide level within a 24-hour period. The present study was therefore able to decipher the potential of BMC to oxidation of a fragmented sulfide concentration in an oxygen deficient condition of an incubator shake flasks using a single nutrient composition under defined operational parameters of pH, temperature, acclimatization time and agitation. Results indicated an overwhelming oxidation of varying sulfide concentration which is an energy source for biomass synthesis. This correspond to an average cell biomass increase at optical density greater than one after 6 hrs of seeding, which later almost doubled at 24 hrs period. Furthermore, BMC degraded on average, 97.50%, 96.67% and 93% of the different concentrations of sulfide in 24 hrs of inoculation, respectively. Sulfide oxidation and uptake were in ascending order 200 ppm S2- L1- d1- > 300 ppm S2- L1- d1- > 500, ppm S2- L1- d1-. Overall, the result signifies the synergistic complementary effects of helper bacteria in the consortium which lessen the oxidative stress, thus ensuring the growth and removal in both isolates. The mixed-culture consortium, S1 and S2, showcased an expeditious growth and sulfide degradation in chemically deficient medium and operational parameters of shake flasks, demonstrating that the consortium would be applicable to handle wastewater laden with H2S.. Penerbit Akademia Baru 2017-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56288/1/Ahmad%20et%20al%202017.pdf Ahmad, Mani Malam and Mohd Azoddein, Abd. Aziz and Mohd Zahari, Mior Ahmad Khusairi and Abu Seman, Mazrul Nizam and Jami, Mohammed Saedi (2017) Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth. Journal of Advanced Research in Materials Science, 30 (1). pp. 10-20. E-ISSN 2289-7992 http://www.akademiabaru.com/doc/ARMSV30_N1_P10_20.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
description	The inhibitory effect of hydrogen sulfide has been indicated to activate a sequence of response mechanism through microbial mutual interactions. Bacterial mixed-culture (BMC) consortium has been found to reduce the complexity of oxidative stress through mutual interactions of helper bacteria thereby facilitating the growth of the disadvantaged inoculum. The potential sulfide bioxidation of strain one (S1) and strain two (S2) mixed-culture bacterial consortiums were tested at different sulfide level within a 24-hour period. The present study was therefore able to decipher the potential of BMC to oxidation of a fragmented sulfide concentration in an oxygen deficient condition of an incubator shake flasks using a single nutrient composition under defined operational parameters of pH, temperature, acclimatization time and agitation. Results indicated an overwhelming oxidation of varying sulfide concentration which is an energy source for biomass synthesis. This correspond to an average cell biomass increase at optical density greater than one after 6 hrs of seeding, which later almost doubled at 24 hrs period. Furthermore, BMC degraded on average, 97.50%, 96.67% and 93% of the different concentrations of sulfide in 24 hrs of inoculation, respectively. Sulfide oxidation and uptake were in ascending order 200 ppm S2- L1- d1- > 300 ppm S2- L1- d1- > 500, ppm S2- L1- d1-. Overall, the result signifies the synergistic complementary effects of helper bacteria in the consortium which lessen the oxidative stress, thus ensuring the growth and removal in both isolates. The mixed-culture consortium, S1 and S2, showcased an expeditious growth and sulfide degradation in chemically deficient medium and operational parameters of shake flasks, demonstrating that the consortium would be applicable to handle wastewater laden with H2S..
format	Article
author	Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi
author_facet	Ahmad, Mani Malam Mohd Azoddein, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi
author_sort	Ahmad, Mani Malam
title	Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
title_short	Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
title_full	Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
title_fullStr	Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
title_full_unstemmed	Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth
title_sort	microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (bmc) growth
publisher	Penerbit Akademia Baru
publishDate	2017
url	http://irep.iium.edu.my/56288/ http://irep.iium.edu.my/56288/ http://irep.iium.edu.my/56288/1/Ahmad%20et%20al%202017.pdf
first_indexed	2023-09-18T21:19:23Z
last_indexed	2023-09-18T21:19:23Z
_version_	1777411778909569024

Microbial interactions in response to sulfide effect on mesophilic bacterial mixed culture (BMC) growth

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