Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model

Obesity is accumulation of excess body fat to the extent it may have an adverse effect on health. It is undeniably a complex disease with risk factors combination of genetics, lifestyle, nutrition, increasing age and lack of physical activity. Alarming increase of obesity prevalence since past few y...

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Main Authors:	Hamidon, Hanisuhana, Bakhtiar, M. Taher, Darnis, Deny Susanti, Ahmad Rusmili, Muhamad Rusdi
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/56145/ http://irep.iium.edu.my/56145/17/56145.pdf

id	iium-56145
recordtype	eprints
spelling	iium-561452017-04-18T06:28:06Z http://irep.iium.edu.my/56145/ Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti Ahmad Rusmili, Muhamad Rusdi RS Pharmacy and materia medica Obesity is accumulation of excess body fat to the extent it may have an adverse effect on health. It is undeniably a complex disease with risk factors combination of genetics, lifestyle, nutrition, increasing age and lack of physical activity. Alarming increase of obesity prevalence since past few years brings the concern to explore effective therapy against obesity. Thus, targeting preadipocyte differentiation (adipogenesis) is one of natural product’s approaches to exert its anti-obesity property. The success of other Garcinia species for obesity management opened the gap to study the lipid alteration potential the locally underexplored Garcinia atroviridis or Asam Gelugur on 3T3-L1 cell culture model. The fresh fruit was extracted and freeze-dried. We first found that the powdered extract did not affect cell viability of both preadipocyte and adipocyte cells. The extract significantly inhibited adipogenesis by decreasing intracellular lipid accumulation as analysed qualitatively and quantitatively using Oil Red O stain. We further investigated the inhibition effect by means of quantifying the protein associated with adipogenesis, PPARγ and C/EBPα. The treatment only affects the expression of C/EBPα protein significantly. Furthermore, the extract was also enhanced the adipocytes breakdown (adipolysis) as revealed by glycerol concentration measured. Taken together, our findings indicated that the fruit extract of G. atroviridis plays a role in alteration of lipid accumulation with the intervention of protein associated with adipogenesis. Understanding adipogenesis and its molecular mechanisms may provide clues for future strategies development in preventing and treating obesity. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/56145/17/56145.pdf Hamidon, Hanisuhana and Bakhtiar, M. Taher and Darnis, Deny Susanti and Ahmad Rusmili, Muhamad Rusdi (2017) Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model. In: International Conference on Natural Products 2017, 15th-16th March 2017, Lumut, Perak. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti Ahmad Rusmili, Muhamad Rusdi Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
description	Obesity is accumulation of excess body fat to the extent it may have an adverse effect on health. It is undeniably a complex disease with risk factors combination of genetics, lifestyle, nutrition, increasing age and lack of physical activity. Alarming increase of obesity prevalence since past few years brings the concern to explore effective therapy against obesity. Thus, targeting preadipocyte differentiation (adipogenesis) is one of natural product’s approaches to exert its anti-obesity property. The success of other Garcinia species for obesity management opened the gap to study the lipid alteration potential the locally underexplored Garcinia atroviridis or Asam Gelugur on 3T3-L1 cell culture model. The fresh fruit was extracted and freeze-dried. We first found that the powdered extract did not affect cell viability of both preadipocyte and adipocyte cells. The extract significantly inhibited adipogenesis by decreasing intracellular lipid accumulation as analysed qualitatively and quantitatively using Oil Red O stain. We further investigated the inhibition effect by means of quantifying the protein associated with adipogenesis, PPARγ and C/EBPα. The treatment only affects the expression of C/EBPα protein significantly. Furthermore, the extract was also enhanced the adipocytes breakdown (adipolysis) as revealed by glycerol concentration measured. Taken together, our findings indicated that the fruit extract of G. atroviridis plays a role in alteration of lipid accumulation with the intervention of protein associated with adipogenesis. Understanding adipogenesis and its molecular mechanisms may provide clues for future strategies development in preventing and treating obesity.
format	Conference or Workshop Item
author	Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti Ahmad Rusmili, Muhamad Rusdi
author_facet	Hamidon, Hanisuhana Bakhtiar, M. Taher Darnis, Deny Susanti Ahmad Rusmili, Muhamad Rusdi
author_sort	Hamidon, Hanisuhana
title	Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
title_short	Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
title_full	Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
title_fullStr	Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
title_full_unstemmed	Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model
title_sort	garcinia atroviridis fruit extract alters lipid accumulation in 3t3-l1 cell line model
publishDate	2017
url	http://irep.iium.edu.my/56145/ http://irep.iium.edu.my/56145/17/56145.pdf
first_indexed	2023-09-18T21:19:13Z
last_indexed	2023-09-18T21:19:13Z
_version_	1777411769103286272

Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model

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