Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains

A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The...

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Main Authors: Alam, Md. Zahangir, Drani, Afifah, Muyibi, Suleyman Aremu
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/5583/
http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf
id iium-5583
recordtype eprints
spelling iium-55832012-01-17T04:05:58Z http://irep.iium.edu.my/5583/ Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains Alam, Md. Zahangir Drani, Afifah Muyibi, Suleyman Aremu TP Chemical technology TP248.13 Biotechnology A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains. 2010-04-23 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf Alam, Md. Zahangir and Drani, Afifah and Muyibi, Suleyman Aremu (2010) Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains. In: International Conference on Environmental Science and Technology (ICEST 2010) , 23-25 April 2010, Bangkok, Thailand. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Drani, Afifah
Muyibi, Suleyman Aremu
Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
description A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains.
format Conference or Workshop Item
author Alam, Md. Zahangir
Drani, Afifah
Muyibi, Suleyman Aremu
author_facet Alam, Md. Zahangir
Drani, Afifah
Muyibi, Suleyman Aremu
author_sort Alam, Md. Zahangir
title Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
title_short Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
title_full Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
title_fullStr Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
title_full_unstemmed Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
title_sort domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
publishDate 2010
url http://irep.iium.edu.my/5583/
http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf
first_indexed 2023-09-18T20:14:15Z
last_indexed 2023-09-18T20:14:15Z
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