Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge

An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that t...

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Main Authors:	Drani , Afifah, Alam, Md. Zahangir, Muyibi, Suleyman Aremu
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf

id	iium-5576
recordtype	eprints
spelling	iium-55762011-11-22T00:26:16Z http://irep.iium.edu.my/5576/ Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu TP Chemical technology TP248.13 Biotechnology An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that the optimum media constituent for cellulase production using sewage sludge as the major substrate, containing 1.5% cellulose and 0.5% peptone, supplemented with 0.25% KH2PO4, 0.2% tween 80, and 0.03% MgSO4 gave the maximum cellulase activity of 21.33 U/ml (CMCase activity). Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.92 with very low probability value (p < 0.0001) for the developed model. The present model is a good prediction of the experimental results and it is highly significant. 2009-05-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf Drani , Afifah and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2009) Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge. In: Water Malaysia 2009 Conference & Exhibition, 19-21 May 2009, Kuala Lumpur. (Unpublished) http://www.watermalaysia.com/index.php?option=com_content&view=article&id=13:welcome-to-watermalaysiacom&catid=13:example-category
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
description	An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that the optimum media constituent for cellulase production using sewage sludge as the major substrate, containing 1.5% cellulose and 0.5% peptone, supplemented with 0.25% KH2PO4, 0.2% tween 80, and 0.03% MgSO4 gave the maximum cellulase activity of 21.33 U/ml (CMCase activity). Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.92 with very low probability value (p < 0.0001) for the developed model. The present model is a good prediction of the experimental results and it is highly significant.
format	Conference or Workshop Item
author	Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu
author_facet	Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu
author_sort	Drani , Afifah
title	Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
title_short	Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
title_full	Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
title_fullStr	Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
title_full_unstemmed	Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
title_sort	sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
publishDate	2009
url	http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf
first_indexed	2023-09-18T20:14:14Z
last_indexed	2023-09-18T20:14:14Z
_version_	1777407680698122240

Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge

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