Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application

The use of synthetically derived poly(lactic-co-glycolic acid) scaffold and naturally derived materials in regeneration of intervertebral disks has been reported in many previous studies. However, the potential effect of poly(lactic-co-glycolic acid) in combination with atelocollagen or fibrin or b...

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Main Authors:	Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Harun Ismail, Ahmad Fahmi, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Hashim, Rosyafirah, Mat Nawi, Nur Farhana, Zainol, Ismail, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format:	Article
Language:	English English English
Published:	SAGE Journals 2017
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/1/MYM%20ET%20AL.pdf http://irep.iium.edu.my/55723/2/Fabrication%20and%20characterization%20of%20three-dimensional%20poly%28lactic%20acid-co-glycolic%20acid%29%2C%20atelocollagen%2C%20and%20fibrin%20bioscaffold%20composite%20for%20intervertebral%20disk%20tissue%20engineering%20application.pdf http://irep.iium.edu.my/55723/8/55723_Fabrication%20and%20characterization%20of%20three-dimensional%20_article.pdf

id	iium-55723
recordtype	eprints
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Harun Ismail, Ahmad Fahmi Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Mat Nawi, Nur Farhana Zainol, Ismail Zulkifly, Ahmad Hafiz Sha'ban, Munirah Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
description	The use of synthetically derived poly(lactic-co-glycolic acid) scaffold and naturally derived materials in regeneration of intervertebral disks has been reported in many previous studies. However, the potential effect of poly(lactic-co-glycolic acid) in combination with atelocollagen or fibrin or both atelocollagen and fibrin bioscaffold composite have not been mentioned so far. This study aims to fabricate and characterize three-dimensional poly(lactic-co-glycolic acid) scaffold incorporated with (1) atelocollagen, (2) fibrin, and (3) both atelocollagen and fibrin combination for intervertebral disk tissue engineering application. The poly(lactic-co-glycolic acid) without anynatural, bioscaffold composites was used as control. The chemical conformation, morphology, cell–scaffold attachment, porosity, water uptake capacity, thermal properties, mechanical strength, and pH level were evaluated on all scaffolds using attenuated total reflectance Fourier transform infrared, scanning electron microscope, gravimetric analysis, swelling test, differential scanning calorimetry, and Instron E3000, respectively. Biocompatibility test was conducted to assess the intervertebral disk, annulus fibrosus cells viability using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay. The attenuated total reflectance Fourier transform infrared results demonstrated notable peaks of amide bond suggesting interaction of atelocollagen, fibrin, and both atelocollagen and fibrin combination into the poly(lactic-co-glycolic acid) scaffold. Based on the scanning electron microscope observation, the pore size of the poly(lactic-co-glycolic acid) structure significantly reduced when it was incorporated with atelocollagen and fibrin. The poly(lactic-co-glycolic acid)–atelocollagen scaffolds demonstrated higher significant swelling ratios, mechanical strength, and thermal stability than the poly(lactic-co-glycolic acid) scaffold alone. All the three bioscaffold composite groups exhibited the ability to reduce the acidic poly(lactic-co-glycolic acid) by-product. In this study, the biocompatibility assessment using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide cells proliferation assay demonstrated a significantly higher annulus fibrosus cells viability in poly(lactic-co-glycolic acid)–atelocollagen–fibrin compared to poly(lactic-co-glycolic acid) alone. The cellular attachment is comparable in poly(lactic-co-glycolic acid)–atelocollagen–fibrin and poly(lactic-co-glycolic acid)–fibrin scaffolds. Overall, these results may suggest potential use of poly(lactic-co-glycolic acid) combined with atelocollagen and fibrin bioscaffold composite for intervertebral disk regeneration.
format	Article
author	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Harun Ismail, Ahmad Fahmi Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Mat Nawi, Nur Farhana Zainol, Ismail Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_facet	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Harun Ismail, Ahmad Fahmi Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Mat Nawi, Nur Farhana Zainol, Ismail Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_sort	Mohamad, Mohd Yusof
title	Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
title_short	Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
title_full	Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
title_fullStr	Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
title_full_unstemmed	Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
title_sort	fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
publisher	SAGE Journals
publishDate	2017
url	http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/ http://irep.iium.edu.my/55723/1/MYM%20ET%20AL.pdf http://irep.iium.edu.my/55723/2/Fabrication%20and%20characterization%20of%20three-dimensional%20poly%28lactic%20acid-co-glycolic%20acid%29%2C%20atelocollagen%2C%20and%20fibrin%20bioscaffold%20composite%20for%20intervertebral%20disk%20tissue%20engineering%20application.pdf http://irep.iium.edu.my/55723/8/55723_Fabrication%20and%20characterization%20of%20three-dimensional%20_article.pdf
first_indexed	2023-09-18T21:18:41Z
last_indexed	2023-09-18T21:18:41Z
_version_	1777411735591845888
spelling	iium-557232018-03-21T00:12:55Z http://irep.iium.edu.my/55723/ Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Harun Ismail, Ahmad Fahmi Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Mat Nawi, Nur Farhana Zainol, Ismail Zulkifly, Ahmad Hafiz Sha'ban, Munirah TA164 Bioengineering The use of synthetically derived poly(lactic-co-glycolic acid) scaffold and naturally derived materials in regeneration of intervertebral disks has been reported in many previous studies. However, the potential effect of poly(lactic-co-glycolic acid) in combination with atelocollagen or fibrin or both atelocollagen and fibrin bioscaffold composite have not been mentioned so far. This study aims to fabricate and characterize three-dimensional poly(lactic-co-glycolic acid) scaffold incorporated with (1) atelocollagen, (2) fibrin, and (3) both atelocollagen and fibrin combination for intervertebral disk tissue engineering application. The poly(lactic-co-glycolic acid) without anynatural, bioscaffold composites was used as control. The chemical conformation, morphology, cell–scaffold attachment, porosity, water uptake capacity, thermal properties, mechanical strength, and pH level were evaluated on all scaffolds using attenuated total reflectance Fourier transform infrared, scanning electron microscope, gravimetric analysis, swelling test, differential scanning calorimetry, and Instron E3000, respectively. Biocompatibility test was conducted to assess the intervertebral disk, annulus fibrosus cells viability using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay. The attenuated total reflectance Fourier transform infrared results demonstrated notable peaks of amide bond suggesting interaction of atelocollagen, fibrin, and both atelocollagen and fibrin combination into the poly(lactic-co-glycolic acid) scaffold. Based on the scanning electron microscope observation, the pore size of the poly(lactic-co-glycolic acid) structure significantly reduced when it was incorporated with atelocollagen and fibrin. The poly(lactic-co-glycolic acid)–atelocollagen scaffolds demonstrated higher significant swelling ratios, mechanical strength, and thermal stability than the poly(lactic-co-glycolic acid) scaffold alone. All the three bioscaffold composite groups exhibited the ability to reduce the acidic poly(lactic-co-glycolic acid) by-product. In this study, the biocompatibility assessment using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide cells proliferation assay demonstrated a significantly higher annulus fibrosus cells viability in poly(lactic-co-glycolic acid)–atelocollagen–fibrin compared to poly(lactic-co-glycolic acid) alone. The cellular attachment is comparable in poly(lactic-co-glycolic acid)–atelocollagen–fibrin and poly(lactic-co-glycolic acid)–fibrin scaffolds. Overall, these results may suggest potential use of poly(lactic-co-glycolic acid) combined with atelocollagen and fibrin bioscaffold composite for intervertebral disk regeneration. SAGE Journals 2017-02-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55723/1/MYM%20ET%20AL.pdf application/pdf en http://irep.iium.edu.my/55723/2/Fabrication%20and%20characterization%20of%20three-dimensional%20poly%28lactic%20acid-co-glycolic%20acid%29%2C%20atelocollagen%2C%20and%20fibrin%20bioscaffold%20composite%20for%20intervertebral%20disk%20tissue%20engineering%20application.pdf application/pdf en http://irep.iium.edu.my/55723/8/55723_Fabrication%20and%20characterization%20of%20three-dimensional%20_article.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Harun Ismail, Ahmad Fahmi and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Hashim, Rosyafirah and Mat Nawi, Nur Farhana and Zainol, Ismail and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2017) Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application. Journal of Bioactive and Compatible Polymers. pp. 1-13. ISSN 0883-9115 E-ISSN 1530-8030 (In Press) http://journals.sagepub.com/doi/10.1177/0883911516686091 10.1177/0883911516686091

Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application

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