Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase

The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphat...

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Main Authors:	Rostam, Muhamad Ashraf, Hassan, Zuridah
Format:	Conference or Workshop Item
Language:	English
Published:	University Publication Centre (UPENA) UiTM 2011
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/55399/ http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf

id	iium-55399
recordtype	eprints
spelling	iium-553992017-04-20T01:59:05Z http://irep.iium.edu.my/55399/ Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase Rostam, Muhamad Ashraf Hassan, Zuridah R Medicine (General) The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphate poisoning using β-glucuronidase assay as an alternative biomarker. A total of 180 blood samples (74 controls and 106 subjects) were examined for this enzyme using a spectrophotometer. These results were then compared to the routine screening assay, butyrylcholinesterase (BChE) assay using a chemical analyzer. Both results showed absence of organophosphate poisoning indicated by no increased in β-glucuronidase level with no reduced in BChE level. Results of BChE level in the subject group (7879.70 ±227.935 IU/L), was significantly higher than the control group (7067.86 ±1609.712 IU/L) (p<0.05). Meanwhile, β- glucuronidase level between the subject (194.48 ±114.711 U/dL) and control group (192.60 ±122.685 U/dL) showed no significant difference (p>0.05). The findings of this evaluation show that assaying for the β-glucuronidase enzyme can be an alternative method to determine organophosphate poisoning. Furthermore, β-glucuronidase assay using spectrophotometer is recommended due to its ease of use and suitability for small laboratories with limited facilities. University Publication Centre (UPENA) UiTM 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf Rostam, Muhamad Ashraf and Hassan, Zuridah (2011) Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase. In: UiTM Sarawak Conference 2011, Kuching, Sarawak.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Rostam, Muhamad Ashraf Hassan, Zuridah Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
description	The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphate poisoning using β-glucuronidase assay as an alternative biomarker. A total of 180 blood samples (74 controls and 106 subjects) were examined for this enzyme using a spectrophotometer. These results were then compared to the routine screening assay, butyrylcholinesterase (BChE) assay using a chemical analyzer. Both results showed absence of organophosphate poisoning indicated by no increased in β-glucuronidase level with no reduced in BChE level. Results of BChE level in the subject group (7879.70 ±227.935 IU/L), was significantly higher than the control group (7067.86 ±1609.712 IU/L) (p<0.05). Meanwhile, β- glucuronidase level between the subject (194.48 ±114.711 U/dL) and control group (192.60 ±122.685 U/dL) showed no significant difference (p>0.05). The findings of this evaluation show that assaying for the β-glucuronidase enzyme can be an alternative method to determine organophosphate poisoning. Furthermore, β-glucuronidase assay using spectrophotometer is recommended due to its ease of use and suitability for small laboratories with limited facilities.
format	Conference or Workshop Item
author	Rostam, Muhamad Ashraf Hassan, Zuridah
author_facet	Rostam, Muhamad Ashraf Hassan, Zuridah
author_sort	Rostam, Muhamad Ashraf
title	Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
title_short	Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
title_full	Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
title_fullStr	Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
title_full_unstemmed	Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
title_sort	assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
publisher	University Publication Centre (UPENA) UiTM
publishDate	2011
url	http://irep.iium.edu.my/55399/ http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf
first_indexed	2023-09-18T21:18:18Z
last_indexed	2023-09-18T21:18:18Z
_version_	1777411710753177600

Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase

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