Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil

Aims: Research on lignin degradation capability is previously restricted exclusively to fungal enzymes. However, recent studies had successfully revealed several soil bacterial strains that were able to produce ligninolytic enzymes. These bacterial ligninolytic enzymes were claimed to be more specif...

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Main Authors:	Mohamad Roslan, Muhamad Aidilfitri, Zainal Abidin, Zaima Azira, Mohd Omar, Suhaila
Format:	Article
Language:	English English
Published:	Malaysian Society for Microbiology 2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/55348/ http://irep.iium.edu.my/55348/ http://irep.iium.edu.my/55348/1/5.%20Screening%20of%20ligninase-producing%20bacteria%20from%20south%20east%20Pahang%20peat%20swamp%20forest%20soil.pdf http://irep.iium.edu.my/55348/7/55348_Screening%20of%20ligninase-producing%20bacteria_SCOPUS.pdf

id	iium-55348
recordtype	eprints
spelling	iium-553482017-07-04T08:31:58Z http://irep.iium.edu.my/55348/ Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila QR Microbiology Aims: Research on lignin degradation capability is previously restricted exclusively to fungal enzymes. However, recent studies had successfully revealed several soil bacterial strains that were able to produce ligninolytic enzymes. These bacterial ligninolytic enzymes were claimed to be more specific in catalysing cleavage of certain linkages between phenolic units of lignin polymers as compared to fungal enzymes. The present study focuses on screening for ligninase- producing bacteria isolated from South East Pahang Peat Swamp Forest (SEPPSF) soil using agar-based assay. Methodology and results: Thirteen isolates used in this study, which were selected based on distinctive colony morphology from our previous isolation work, showed decolourisation zone on Azure B plates screening. The ratio of decolourisation zones were measured to the ratio of the colony size and the biggest ratio was 2.22 by isolate AR1. Only 4 out of the 13 isolates were able to grow on lignin plates. Subsequently, the 4 isolates, AR3, AR8, AR10 and AR13 were tested on M1 agar supplemented with 3 ligninolytic enzyme indicator compounds which were tannic acid (TA), guaiacol and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) respectively. All four isolates showed growth on TA plates while only AR10 showed a clear brown coloration. An Intense reddish-brown colour formation was observed around the colony of isolates AR3 and AR10 on guaiacol plates while none exhibited green coloration around the colonies when tested on ABTS plates. Conclusions, significance and impacts of study: Isolate AR10 that was identified as Serratia sp. was perceived to be a potential ligninase-producer, though in-depth analysis has to be conducted in the future to determine the specific ligninolytic enzyme activities and characteristics. The application of different substrates is essential to investigate the ligninolytic potential and reaction of those bacterial enzymes towards different indicator compounds. This study is a preliminary endeavour concerning potential ligninolytic enzymes from bacteria as biocatalysts in various industrial processes. This is the first report on preliminary study for ligninolytic activities of soil bacteria from SEPPSF soil. Keywords: ligninolytic enzymes, bacteria, peat swamp forest, tannic acid, guaiacol Malaysian Society for Microbiology 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55348/1/5.%20Screening%20of%20ligninase-producing%20bacteria%20from%20south%20east%20Pahang%20peat%20swamp%20forest%20soil.pdf application/pdf en http://irep.iium.edu.my/55348/7/55348_Screening%20of%20ligninase-producing%20bacteria_SCOPUS.pdf Mohamad Roslan, Muhamad Aidilfitri and Zainal Abidin, Zaima Azira and Mohd Omar, Suhaila (2016) Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil. Malaysian Journal of Microbiology, 12 (6). pp. 433-437. ISSN 1823-8262 E-ISSN 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
description	Aims: Research on lignin degradation capability is previously restricted exclusively to fungal enzymes. However, recent studies had successfully revealed several soil bacterial strains that were able to produce ligninolytic enzymes. These bacterial ligninolytic enzymes were claimed to be more specific in catalysing cleavage of certain linkages between phenolic units of lignin polymers as compared to fungal enzymes. The present study focuses on screening for ligninase- producing bacteria isolated from South East Pahang Peat Swamp Forest (SEPPSF) soil using agar-based assay. Methodology and results: Thirteen isolates used in this study, which were selected based on distinctive colony morphology from our previous isolation work, showed decolourisation zone on Azure B plates screening. The ratio of decolourisation zones were measured to the ratio of the colony size and the biggest ratio was 2.22 by isolate AR1. Only 4 out of the 13 isolates were able to grow on lignin plates. Subsequently, the 4 isolates, AR3, AR8, AR10 and AR13 were tested on M1 agar supplemented with 3 ligninolytic enzyme indicator compounds which were tannic acid (TA), guaiacol and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) respectively. All four isolates showed growth on TA plates while only AR10 showed a clear brown coloration. An Intense reddish-brown colour formation was observed around the colony of isolates AR3 and AR10 on guaiacol plates while none exhibited green coloration around the colonies when tested on ABTS plates. Conclusions, significance and impacts of study: Isolate AR10 that was identified as Serratia sp. was perceived to be a potential ligninase-producer, though in-depth analysis has to be conducted in the future to determine the specific ligninolytic enzyme activities and characteristics. The application of different substrates is essential to investigate the ligninolytic potential and reaction of those bacterial enzymes towards different indicator compounds. This study is a preliminary endeavour concerning potential ligninolytic enzymes from bacteria as biocatalysts in various industrial processes. This is the first report on preliminary study for ligninolytic activities of soil bacteria from SEPPSF soil. Keywords: ligninolytic enzymes, bacteria, peat swamp forest, tannic acid, guaiacol
format	Article
author	Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_facet	Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_sort	Mohamad Roslan, Muhamad Aidilfitri
title	Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
title_short	Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
title_full	Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
title_fullStr	Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
title_full_unstemmed	Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil
title_sort	screening of ligninase-producing bacteria from south east pahang peat swamp forest soil
publisher	Malaysian Society for Microbiology
publishDate	2016
url	http://irep.iium.edu.my/55348/ http://irep.iium.edu.my/55348/ http://irep.iium.edu.my/55348/1/5.%20Screening%20of%20ligninase-producing%20bacteria%20from%20south%20east%20Pahang%20peat%20swamp%20forest%20soil.pdf http://irep.iium.edu.my/55348/7/55348_Screening%20of%20ligninase-producing%20bacteria_SCOPUS.pdf
first_indexed	2023-09-18T21:18:14Z
last_indexed	2023-09-18T21:18:14Z
_version_	1777411706393198592

Screening of ligninase-producing bacteria from south east Pahang peat swamp forest soil

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