Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium

Chlorinated benzoic acids (CBA) have been intentionally released to the environment due to their use in agriculture as herbicides or pesticides, or unintentionally because they are common metabolites in the aerobic transformation of many chlorinated pollutants. The biodegradation of 3,4-dichlorobe...

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Main Authors:	Alqudah, Ali Abdallah, Tarawneh, Khaled Ahmed, Alkafaween, Ibrahim Khaled, Saad, Shahbudin
Format:	Article
Language:	English
Published:	Canadian Center of Science and Education 2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/8/55270-edited.pdf

id	iium-55270
recordtype	eprints
spelling	iium-552702017-07-15T01:43:06Z http://irep.iium.edu.my/55270/ Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium Alqudah, Ali Abdallah Tarawneh, Khaled Ahmed Alkafaween, Ibrahim Khaled Saad, Shahbudin QR Microbiology Chlorinated benzoic acids (CBA) have been intentionally released to the environment due to their use in agriculture as herbicides or pesticides, or unintentionally because they are common metabolites in the aerobic transformation of many chlorinated pollutants. The biodegradation of 3,4-dichlorobenzoic acid compound was investigated by using Corynebacterium jeikeium bacteria, which was isolated from Petra wastewater plant in Jordan. 3,4-dichlorobenzoic acid compound (3,4-DCBA) was used as a sole carbon and energy source in minimal salt media (MSM). 3,4-DCBA was the most degradable compound among the Chlorobenzoic acid compounds tested by this bacteria. Different conditions such as substrate concentration, temperature, pH, agitation rate, carbon starvation and carbon adaptation were used to obtain optimal Biodegradation. The optimal conditions for the biodegradation were 3mM as substrate concentration and the culture conditions were also showed a significant impact on the ability of these cells to remove 3,4-DCBA. The optimum solution, temperature and agitation rate were 7.5, 37 °C and 150 rpm, respectively. Adaptation of the cells to 3,4-DCBA for 24 hr and 48 hr and cells starvation for 24 hr and 48 hr increased the initial degradation rate. The degradation ability was monitored through disappearance of the substrate from the medium and the measuring the accompanying chloride release. Corynebacterium jeikeium dioxygenases were physiologically induced by 3,4-DCBA compound. They were analyzed for both ortho or meta ring-cleavage of this aromatic compound. Only 1,2-dioxygenase activity was detected which mean that the cleavage is through the ortho pathway. This microorganism can be a valuable and promising candidate for use in the biotreatment of wastewater samples contaminated with 3,4-DCBA. Canadian Center of Science and Education 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55270/8/55270-edited.pdf Alqudah, Ali Abdallah and Tarawneh, Khaled Ahmed and Alkafaween, Ibrahim Khaled and Saad, Shahbudin (2014) Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium. International Journal of Biology, 6 (3). pp. 54-63. ISSN 1916-9671 E-ISSN 1916-968X http://www.ccsenet.org/journal/index.php/ijb/article/view/34994/20959 10.5539/ijb.v6n3p54
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Alqudah, Ali Abdallah Tarawneh, Khaled Ahmed Alkafaween, Ibrahim Khaled Saad, Shahbudin Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
description	Chlorinated benzoic acids (CBA) have been intentionally released to the environment due to their use in agriculture as herbicides or pesticides, or unintentionally because they are common metabolites in the aerobic transformation of many chlorinated pollutants. The biodegradation of 3,4-dichlorobenzoic acid compound was investigated by using Corynebacterium jeikeium bacteria, which was isolated from Petra wastewater plant in Jordan. 3,4-dichlorobenzoic acid compound (3,4-DCBA) was used as a sole carbon and energy source in minimal salt media (MSM). 3,4-DCBA was the most degradable compound among the Chlorobenzoic acid compounds tested by this bacteria. Different conditions such as substrate concentration, temperature, pH, agitation rate, carbon starvation and carbon adaptation were used to obtain optimal Biodegradation. The optimal conditions for the biodegradation were 3mM as substrate concentration and the culture conditions were also showed a significant impact on the ability of these cells to remove 3,4-DCBA. The optimum solution, temperature and agitation rate were 7.5, 37 °C and 150 rpm, respectively. Adaptation of the cells to 3,4-DCBA for 24 hr and 48 hr and cells starvation for 24 hr and 48 hr increased the initial degradation rate. The degradation ability was monitored through disappearance of the substrate from the medium and the measuring the accompanying chloride release. Corynebacterium jeikeium dioxygenases were physiologically induced by 3,4-DCBA compound. They were analyzed for both ortho or meta ring-cleavage of this aromatic compound. Only 1,2-dioxygenase activity was detected which mean that the cleavage is through the ortho pathway. This microorganism can be a valuable and promising candidate for use in the biotreatment of wastewater samples contaminated with 3,4-DCBA.
format	Article
author	Alqudah, Ali Abdallah Tarawneh, Khaled Ahmed Alkafaween, Ibrahim Khaled Saad, Shahbudin
author_facet	Alqudah, Ali Abdallah Tarawneh, Khaled Ahmed Alkafaween, Ibrahim Khaled Saad, Shahbudin
author_sort	Alqudah, Ali Abdallah
title	Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
title_short	Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
title_full	Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
title_fullStr	Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
title_full_unstemmed	Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium
title_sort	optimizing the biodegradation of 3,4-dichlorobenzoic acid by corynebacterium jeikeium
publisher	Canadian Center of Science and Education
publishDate	2014
url	http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/ http://irep.iium.edu.my/55270/8/55270-edited.pdf
first_indexed	2023-09-18T21:18:08Z
last_indexed	2023-09-18T21:18:08Z
_version_	1777411700206600192

Optimizing the biodegradation of 3,4-Dichlorobenzoic acid by Corynebacterium jeikeium

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