Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate

Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate

A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The...

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Main Authors: Alam, Md. Zahangir, Mansor, Mariatul F., Drani , Afifah, Muyibi, Suleyman Aremu, Abdul Karim, Mohamed Ismail, Hamzah, Mohd. Salleh, Kabbashi, Nassereldeen Ahmed
Format: Conference or Workshop Item
Language:English
Published: 2008
Subjects:
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/5523/
http://irep.iium.edu.my/5523/1/Full-paper-Indonesia.pdf
id iium-5523
recordtype eprints
spelling iium-55232012-08-08T07:07:49Z http://irep.iium.edu.my/5523/ Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate Alam, Md. Zahangir Mansor, Mariatul F. Drani , Afifah Muyibi, Suleyman Aremu Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh Kabbashi, Nassereldeen Ahmed TP Chemical technology TP248.13 Biotechnology A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains. 2008-10-29 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5523/1/Full-paper-Indonesia.pdf Alam, Md. Zahangir and Mansor, Mariatul F. and Drani , Afifah and Muyibi, Suleyman Aremu and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh and Kabbashi, Nassereldeen Ahmed (2008) Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate. In: The Sixth International Symposium on Southeast Asian Water Environment, 29 - 31 October 2008, Bandung, Indonesia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Mansor, Mariatul F.
Drani , Afifah
Muyibi, Suleyman Aremu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
Kabbashi, Nassereldeen Ahmed
Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
description A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains.
format Conference or Workshop Item
author Alam, Md. Zahangir
Mansor, Mariatul F.
Drani , Afifah
Muyibi, Suleyman Aremu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
Kabbashi, Nassereldeen Ahmed
author_facet Alam, Md. Zahangir
Mansor, Mariatul F.
Drani , Afifah
Muyibi, Suleyman Aremu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
Kabbashi, Nassereldeen Ahmed
author_sort Alam, Md. Zahangir
title Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
title_short Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
title_full Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
title_fullStr Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
title_full_unstemmed Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
title_sort comparison of trichoderma reesei rut c-30 and locally isolated aspergillus spp. cellulases using domestic wastewater sludge as a major substrate
publishDate 2008
url http://irep.iium.edu.my/5523/
http://irep.iium.edu.my/5523/1/Full-paper-Indonesia.pdf
first_indexed 2023-09-18T20:14:10Z
last_indexed 2023-09-18T20:14:10Z
_version_ 1777407675895644160

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