Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549

Introduction: Lung cancer has been reported as one of the most common types of cancer worldwide. Current cancer treatments like chemotherapy do not result in a complete cure and are known to cause side effects in the patients. Therefore, alternative treatment strategies are being explored, one of wh...

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Main Authors:	Sirajudeen, Azila, Mohd Tahir, Aisyah Hanani, Abdul Ghani, Radiah
Format:	Article
Language:	English
Published:	International Islamic University Malaysia, Kuantan. 2016
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/55205/ http://irep.iium.edu.my/55205/ http://irep.iium.edu.my/55205/1/MRS2016-ABSTRACT-BOOK-57.pdf

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recordtype	eprints
spelling	iium-552052017-07-18T01:34:48Z http://irep.iium.edu.my/55205/ Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549 Sirajudeen, Azila Mohd Tahir, Aisyah Hanani Abdul Ghani, Radiah RB Pathology Introduction: Lung cancer has been reported as one of the most common types of cancer worldwide. Current cancer treatments like chemotherapy do not result in a complete cure and are known to cause side effects in the patients. Therefore, alternative treatment strategies are being explored, one of which is to investigate the potential of the local herbs in this regard. Piper sarmentosum (daun kaduk) has received much attention due to its anti-cancer properties in A549 cells. In this study, the cell cycle profile and mechanisms of cell death induced by P. sarmentosum were investigated using a flow cytometer. Methods: The cell cycle profile changes were observed using propidium iodide staining while the type of cell death was analyzed using Annexin-V assay. Caspases -3/7,8 and 9 and cytochrome c assays were elucidated using flow cytometry analysis. Results: P.sarmentosum arrested the growth of A549 cells at G0/G1 phase. The Annexin V analysis revealed that P. sarmentosum exhibited significant induction of apoptosis after 24 h exposure. Caspases analysis showed that P. sarmentosum induced apoptosis through mitochondrial pathway, via the activation of caspase 3 and caspase 9. Meanwhile, cytochrome c analysis revealed that P. sarmentosum induced a mitochondrial pathway of cell death through the release of cytochrome c. Conclusions: Based on these preliminary findings, P. sarmentosum has a great potential as a dietary cancer treatment for lung cancer and may perhaps be used for lung cancer pharmacotherapy in the clinical settings in future. International Islamic University Malaysia, Kuantan. 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55205/1/MRS2016-ABSTRACT-BOOK-57.pdf Sirajudeen, Azila and Mohd Tahir, Aisyah Hanani and Abdul Ghani, Radiah (2016) Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549. International Medical Journal of Malaysia (IMJM), 15 (Supplemental issue). p. 57. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-57.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Sirajudeen, Azila Mohd Tahir, Aisyah Hanani Abdul Ghani, Radiah Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
description	Introduction: Lung cancer has been reported as one of the most common types of cancer worldwide. Current cancer treatments like chemotherapy do not result in a complete cure and are known to cause side effects in the patients. Therefore, alternative treatment strategies are being explored, one of which is to investigate the potential of the local herbs in this regard. Piper sarmentosum (daun kaduk) has received much attention due to its anti-cancer properties in A549 cells. In this study, the cell cycle profile and mechanisms of cell death induced by P. sarmentosum were investigated using a flow cytometer. Methods: The cell cycle profile changes were observed using propidium iodide staining while the type of cell death was analyzed using Annexin-V assay. Caspases -3/7,8 and 9 and cytochrome c assays were elucidated using flow cytometry analysis. Results: P.sarmentosum arrested the growth of A549 cells at G0/G1 phase. The Annexin V analysis revealed that P. sarmentosum exhibited significant induction of apoptosis after 24 h exposure. Caspases analysis showed that P. sarmentosum induced apoptosis through mitochondrial pathway, via the activation of caspase 3 and caspase 9. Meanwhile, cytochrome c analysis revealed that P. sarmentosum induced a mitochondrial pathway of cell death through the release of cytochrome c. Conclusions: Based on these preliminary findings, P. sarmentosum has a great potential as a dietary cancer treatment for lung cancer and may perhaps be used for lung cancer pharmacotherapy in the clinical settings in future.
format	Article
author	Sirajudeen, Azila Mohd Tahir, Aisyah Hanani Abdul Ghani, Radiah
author_facet	Sirajudeen, Azila Mohd Tahir, Aisyah Hanani Abdul Ghani, Radiah
author_sort	Sirajudeen, Azila
title	Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_short	Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_full	Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_fullStr	Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_full_unstemmed	Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549
title_sort	piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, a549
publisher	International Islamic University Malaysia, Kuantan.
publishDate	2016
url	http://irep.iium.edu.my/55205/ http://irep.iium.edu.my/55205/ http://irep.iium.edu.my/55205/1/MRS2016-ABSTRACT-BOOK-57.pdf
first_indexed	2023-09-18T21:18:03Z
last_indexed	2023-09-18T21:18:03Z
_version_	1777411695246835712

Piper sarmentosum induced apoptosis via mitochondrial pathway in human lung adenocarcinoma cells, A549

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