Crude oil bioremediation by indigenous bacteria isolated from oily sludge

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella...

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Main Authors:	Azizan, Nur Hafizah, Apun, Kasing, Bilung, Lesley Maurice, Vincent, Micky, Roslan, Hairul Azman, Awang Ahmad Husaini, Awang Ahmad Sallehin
Format:	Article
Language:	English English
Published:	UTM Press 2016
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/1/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge.pdf http://irep.iium.edu.my/55114/2/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge_Scopus.pdf

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recordtype	eprints
spelling	iium-551142017-04-18T02:32:07Z http://irep.iium.edu.my/55114/ Crude oil bioremediation by indigenous bacteria isolated from oily sludge Azizan, Nur Hafizah Apun, Kasing Bilung, Lesley Maurice Vincent, Micky Roslan, Hairul Azman Awang Ahmad Husaini, Awang Ahmad Sallehin QH301 Biology Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively. UTM Press 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55114/1/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge.pdf application/pdf en http://irep.iium.edu.my/55114/2/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge_Scopus.pdf Azizan, Nur Hafizah and Apun, Kasing and Bilung, Lesley Maurice and Vincent, Micky and Roslan, Hairul Azman and Awang Ahmad Husaini, Awang Ahmad Sallehin (2016) Crude oil bioremediation by indigenous bacteria isolated from oily sludge. Jurnal Teknologi, 78 (11-2). pp. 61-66. ISSN 0127-9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/9945/5983 10.11113/jt.v78.9945
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QH301 Biology
spellingShingle	QH301 Biology Azizan, Nur Hafizah Apun, Kasing Bilung, Lesley Maurice Vincent, Micky Roslan, Hairul Azman Awang Ahmad Husaini, Awang Ahmad Sallehin Crude oil bioremediation by indigenous bacteria isolated from oily sludge
description	Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.
format	Article
author	Azizan, Nur Hafizah Apun, Kasing Bilung, Lesley Maurice Vincent, Micky Roslan, Hairul Azman Awang Ahmad Husaini, Awang Ahmad Sallehin
author_facet	Azizan, Nur Hafizah Apun, Kasing Bilung, Lesley Maurice Vincent, Micky Roslan, Hairul Azman Awang Ahmad Husaini, Awang Ahmad Sallehin
author_sort	Azizan, Nur Hafizah
title	Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_short	Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_full	Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_fullStr	Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_full_unstemmed	Crude oil bioremediation by indigenous bacteria isolated from oily sludge
title_sort	crude oil bioremediation by indigenous bacteria isolated from oily sludge
publisher	UTM Press
publishDate	2016
url	http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/ http://irep.iium.edu.my/55114/1/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge.pdf http://irep.iium.edu.my/55114/2/55114_Crude%20oil%20bioremediation%20by%20indigenous%20bacteria%20isolated%20from%20oily%20sludge_Scopus.pdf
first_indexed	2023-09-18T21:17:56Z
last_indexed	2023-09-18T21:17:56Z
_version_	1777411687469547520

Crude oil bioremediation by indigenous bacteria isolated from oily sludge

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