Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves

Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinocicepti...

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Main Authors:	Shah Jaois, Erman, Abdul Rahman, Suzanah, Ching, Siew Mooi, Abdul Kadir, Arifah, Mohd. Desa, Mohd. Nasir, Zakaria, Zainul Amirudin
Format:	Article
Language:	English English English
Published:	Sociedade Brasileira de Farmacognosia 2016
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/1/Melastoma%20malabathricum%202016.pdf http://irep.iium.edu.my/55094/7/55094_Possible%20mechanisms%20of%20antinociception_WOS.pdf http://irep.iium.edu.my/55094/8/55094_Possible%20mechanisms%20of%20antinociception_SCOPUS.pdf

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spelling	iium-550942017-07-18T00:22:35Z http://irep.iium.edu.my/55094/ Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves Shah Jaois, Erman Abdul Rahman, Suzanah Ching, Siew Mooi Abdul Kadir, Arifah Mohd. Desa, Mohd. Nasir Zakaria, Zainul Amirudin RM Therapeutics. Pharmacology Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinociception involved using various rats’ models. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The in vivo acetic acid-induced abdominal constriction, formalininduced paw licking, and hot plate tests were used as the models of nociception to evaluate the extract antinociceptive activity. Further studies were carried out to determine the role of opioid and vanilloid receptors, glutamate system and nitric oxide/cyclic guanosine phosphate (NO/cGMP) pathway in modulating the extract antinociceptive activity. From the results obtained, M. malabathricum exhibited significant (p < 0.05) antinociceptive activity in all the chemical- and thermal-induced nociception models. Naloxone (5 mg/kg), a non-selective opioid antagonist, failed to significantly affect the antinociceptive activity of MEMM when assessed using the abdominal constriction-, hot plate- and formalin-induced paw licking-test. M. malabathricum also significantly (p < 0.05) reversed the nociceptive response in capsaicinand glutamate-induced paw licking test. Furthermore, only l-arginine (a nitric oxide precursor) alone, but not, NG-nitro-l-arginine methyl esters (l-NAME;an inhibitor of NO synthase), methylene blue (MB; an inhibitor of cGMP), or their combination thereof, significantly (p <0.05) block the antinociceptive activity of M. malabathricum. In conclusion, M. malabathricum exerted a non-opioid antinociceptive activity at the central and peripheral levels partly via the inhibition of vanilloid receptors and glutamatergic system, and activation of the NO-mediated/cGMP-independent pathway. Sociedade Brasileira de Farmacognosia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55094/1/Melastoma%20malabathricum%202016.pdf application/pdf en http://irep.iium.edu.my/55094/7/55094_Possible%20mechanisms%20of%20antinociception_WOS.pdf application/pdf en http://irep.iium.edu.my/55094/8/55094_Possible%20mechanisms%20of%20antinociception_SCOPUS.pdf Shah Jaois, Erman and Abdul Rahman, Suzanah and Ching, Siew Mooi and Abdul Kadir, Arifah and Mohd. Desa, Mohd. Nasir and Zakaria, Zainul Amirudin (2016) Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves. Brazilian Journal of Pharmacognosy, 26 (5). pp. 586-594. ISSN 0102-695X http://www.sciencedirect.com/science/article/pii/S0102695X16300928 10.1016/j.bjp.2016.01.011
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Shah Jaois, Erman Abdul Rahman, Suzanah Ching, Siew Mooi Abdul Kadir, Arifah Mohd. Desa, Mohd. Nasir Zakaria, Zainul Amirudin Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
description	Melastoma malabathricum L., Melastomaceae, has been traditionally used to relieve diverse pain-related ailments. The objectives of the present study were to determine the antinociceptive activity of methanol extract of M. malabathricum leaves and to elucidate the possible mechanisms of antinociception involved using various rats’ models. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The in vivo acetic acid-induced abdominal constriction, formalininduced paw licking, and hot plate tests were used as the models of nociception to evaluate the extract antinociceptive activity. Further studies were carried out to determine the role of opioid and vanilloid receptors, glutamate system and nitric oxide/cyclic guanosine phosphate (NO/cGMP) pathway in modulating the extract antinociceptive activity. From the results obtained, M. malabathricum exhibited significant (p < 0.05) antinociceptive activity in all the chemical- and thermal-induced nociception models. Naloxone (5 mg/kg), a non-selective opioid antagonist, failed to significantly affect the antinociceptive activity of MEMM when assessed using the abdominal constriction-, hot plate- and formalin-induced paw licking-test. M. malabathricum also significantly (p < 0.05) reversed the nociceptive response in capsaicinand glutamate-induced paw licking test. Furthermore, only l-arginine (a nitric oxide precursor) alone, but not, NG-nitro-l-arginine methyl esters (l-NAME;an inhibitor of NO synthase), methylene blue (MB; an inhibitor of cGMP), or their combination thereof, significantly (p <0.05) block the antinociceptive activity of M. malabathricum. In conclusion, M. malabathricum exerted a non-opioid antinociceptive activity at the central and peripheral levels partly via the inhibition of vanilloid receptors and glutamatergic system, and activation of the NO-mediated/cGMP-independent pathway.
format	Article
author	Shah Jaois, Erman Abdul Rahman, Suzanah Ching, Siew Mooi Abdul Kadir, Arifah Mohd. Desa, Mohd. Nasir Zakaria, Zainul Amirudin
author_facet	Shah Jaois, Erman Abdul Rahman, Suzanah Ching, Siew Mooi Abdul Kadir, Arifah Mohd. Desa, Mohd. Nasir Zakaria, Zainul Amirudin
author_sort	Shah Jaois, Erman
title	Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_short	Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_full	Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_fullStr	Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_full_unstemmed	Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves
title_sort	possible mechanisms of antinociception of methanol extract of melastoma malabathricum leaves
publisher	Sociedade Brasileira de Farmacognosia
publishDate	2016
url	http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/ http://irep.iium.edu.my/55094/1/Melastoma%20malabathricum%202016.pdf http://irep.iium.edu.my/55094/7/55094_Possible%20mechanisms%20of%20antinociception_WOS.pdf http://irep.iium.edu.my/55094/8/55094_Possible%20mechanisms%20of%20antinociception_SCOPUS.pdf
first_indexed	2023-09-18T21:17:54Z
last_indexed	2023-09-18T21:17:54Z
_version_	1777411685740445696

Possible mechanisms of antinociception of methanol extract of Melastoma malabathricum leaves

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