Recovery of antifungal peptides from zophobas morio fabricus

Human has strong natural immunity against fungal infections, nevertherless, fungi can cause diseases when this immunity breaks down, such as in cancer patients, transplant recipients and acquired immunodeficiency syndrome (AIDS) patients. In the last few years, fungal infections have increased stri...

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Main Authors:	Faruck, Mohammad Omer, Yusof, Faridah, Chowdhury, Silvia
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	QR Microbiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/55091/ http://irep.iium.edu.my/55091/ http://irep.iium.edu.my/55091/1/55091_RECOVERY%20OF%20ANTIFUNGAL%20PEPTIDES.pdf

id	iium-55091
recordtype	eprints
spelling	iium-550912017-03-30T02:21:51Z http://irep.iium.edu.my/55091/ Recovery of antifungal peptides from zophobas morio fabricus Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia QR Microbiology TP248.13 Biotechnology Human has strong natural immunity against fungal infections, nevertherless, fungi can cause diseases when this immunity breaks down, such as in cancer patients, transplant recipients and acquired immunodeficiency syndrome (AIDS) patients. In the last few years, fungal infections have increased strikingly and it has been blamed for the rise in the number of deaths in patients with compromised immune systems. Antifungal remedy from the natural sources is preferred because chemical treatments maybe toxic to both fungus as well as human cells. In this study, antifungal peptides has been recovered from whole body extraction of non-immunized final instar larvae of Zophobas morio Fabricus using acidified (triflouroacetic acid) solvent (ethanol and isopropanol). The two different extracts were found active, although at varying degrees, towards four fungi tested, namely, Aspergillus niger, Microsporum canis, Candida albicans and Blastomyces dermatitids. In vitro susceptibility results were observed by ‘Colony Count Method’ and this was then compared and confirmed by two other assay methods, namely ‘Poisoned Agar Assay’ and ‘EUCAST Standard Method or Broth Micro Dilution Method’ of the European Center for Disease Prevention and Control. Results showed that the two different extracts exhibited high activity towards the tested fungi, whereby, isopropanolic extract against C. albicans showed the highest inhibition (82%). Besides, results obtained from the other two assay methods showed almost similar trend in activities. This work may present the first initiative towards recovering antifungal peptides from low cost, easily found insect larvae, whereby it can be potentially developed as peptide-based drug to combat fungal infection. Future work includes purifying, identification and characterization of peptides. 2015-11-19 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/55091/1/55091_RECOVERY%20OF%20ANTIFUNGAL%20PEPTIDES.pdf Faruck, Mohammad Omer and Yusof, Faridah and Chowdhury, Silvia (2015) Recovery of antifungal peptides from zophobas morio fabricus. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished) http://www.acb2015.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology TP248.13 Biotechnology
spellingShingle	QR Microbiology TP248.13 Biotechnology Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia Recovery of antifungal peptides from zophobas morio fabricus
description	Human has strong natural immunity against fungal infections, nevertherless, fungi can cause diseases when this immunity breaks down, such as in cancer patients, transplant recipients and acquired immunodeficiency syndrome (AIDS) patients. In the last few years, fungal infections have increased strikingly and it has been blamed for the rise in the number of deaths in patients with compromised immune systems. Antifungal remedy from the natural sources is preferred because chemical treatments maybe toxic to both fungus as well as human cells. In this study, antifungal peptides has been recovered from whole body extraction of non-immunized final instar larvae of Zophobas morio Fabricus using acidified (triflouroacetic acid) solvent (ethanol and isopropanol). The two different extracts were found active, although at varying degrees, towards four fungi tested, namely, Aspergillus niger, Microsporum canis, Candida albicans and Blastomyces dermatitids. In vitro susceptibility results were observed by ‘Colony Count Method’ and this was then compared and confirmed by two other assay methods, namely ‘Poisoned Agar Assay’ and ‘EUCAST Standard Method or Broth Micro Dilution Method’ of the European Center for Disease Prevention and Control. Results showed that the two different extracts exhibited high activity towards the tested fungi, whereby, isopropanolic extract against C. albicans showed the highest inhibition (82%). Besides, results obtained from the other two assay methods showed almost similar trend in activities. This work may present the first initiative towards recovering antifungal peptides from low cost, easily found insect larvae, whereby it can be potentially developed as peptide-based drug to combat fungal infection. Future work includes purifying, identification and characterization of peptides.
format	Conference or Workshop Item
author	Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia
author_facet	Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia
author_sort	Faruck, Mohammad Omer
title	Recovery of antifungal peptides from zophobas morio fabricus
title_short	Recovery of antifungal peptides from zophobas morio fabricus
title_full	Recovery of antifungal peptides from zophobas morio fabricus
title_fullStr	Recovery of antifungal peptides from zophobas morio fabricus
title_full_unstemmed	Recovery of antifungal peptides from zophobas morio fabricus
title_sort	recovery of antifungal peptides from zophobas morio fabricus
publishDate	2015
url	http://irep.iium.edu.my/55091/ http://irep.iium.edu.my/55091/ http://irep.iium.edu.my/55091/1/55091_RECOVERY%20OF%20ANTIFUNGAL%20PEPTIDES.pdf
first_indexed	2023-09-18T21:17:53Z
last_indexed	2023-09-18T21:17:53Z
_version_	1777411685290606592

Recovery of antifungal peptides from zophobas morio fabricus

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