In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts

The present study was carried out to determine the in vitro antibacterial activity of aqueous and chloroform extracts of the leaves and fruits of Averrhoa bilimbi L. using the standard disc diffusion test. The air-dried leaves and fruits of A. bilimbi were separately soaked in distilled water or chl...

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Main Authors:	Zakaria, Zainul Amiruddin, H, Zaiton, Henie, E. F. P., Mat Jais, Abdul Manan, E. Zainudin, Engku Nuraishah Huda
Format:	Article
Language:	English
Published:	Medwell Journals 2007
Subjects:	Q Science (General) QK Botany QR Microbiology
Online Access:	http://irep.iium.edu.my/55077/ http://irep.iium.edu.my/55077/ http://irep.iium.edu.my/55077/1/maxwell%20paper.pdf

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spelling	iium-550772017-03-20T01:22:23Z http://irep.iium.edu.my/55077/ In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts Zakaria, Zainul Amiruddin H, Zaiton Henie, E. F. P. Mat Jais, Abdul Manan E. Zainudin, Engku Nuraishah Huda Q Science (General) QK Botany QR Microbiology The present study was carried out to determine the in vitro antibacterial activity of aqueous and chloroform extracts of the leaves and fruits of Averrhoa bilimbi L. using the standard disc diffusion test. The air-dried leaves and fruits of A. bilimbi were separately soaked in distilled water or chloroform (1:10; wv-1) for 24 h and the resultant supernatants were filtered. The aqueous extract was freeze-dried while the chloroform extract was evaporated to dryness under reduce pressure to obtained the crude dried extracts, which were prepared in the concentration of 25, 50 and 100 mg mL 1 prior to the antibacterial assay. The results obtained demonstrated the potential of A. bilimbi extracts against some of the Gram-positive and-negative bacteria. The 100 mg mL 1 aqueous extract of A. bilimbi’s leaves and fruits showed positive antibacterial activity against the Gram-positive S. aureus, S. epidermis B. cereus, C. diphteriae with the latter also gave positive effect against K. rhizophila. Both extracts also exhibited positive antibacterial activity against the Gram-negative S. typhi, C. fuendii and A. hydrophila. On the other hand, the 100 mg mL 1 chloroform extracts of A. bilimbi’s leaves and fruits demonstrated antibacterial activity against the Gram-positive S. aureus, S. epidermis, B. cereus, K. rhizophila and C. diphteriae and Gram-negative S. typhi, C. fuendii, A. hydrophila and P. vulgaris. In conclusion, the A. bilimbi leaves and fruits possess potential antibacterial activity that requires thorough study. Medwell Journals 2007 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55077/1/maxwell%20paper.pdf Zakaria, Zainul Amiruddin and H, Zaiton and Henie, E. F. P. and Mat Jais, Abdul Manan and E. Zainudin, Engku Nuraishah Huda (2007) In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts. International Journal of Tropical Medicine, 2 (3). pp. 96-100. ISSN 1816-3319 E-ISSN 1818-779X https://www.medwelljournals.com/abstract/?doi=ijtmed.2007.96.100
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QK Botany QR Microbiology
spellingShingle	Q Science (General) QK Botany QR Microbiology Zakaria, Zainul Amiruddin H, Zaiton Henie, E. F. P. Mat Jais, Abdul Manan E. Zainudin, Engku Nuraishah Huda In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
description	The present study was carried out to determine the in vitro antibacterial activity of aqueous and chloroform extracts of the leaves and fruits of Averrhoa bilimbi L. using the standard disc diffusion test. The air-dried leaves and fruits of A. bilimbi were separately soaked in distilled water or chloroform (1:10; wv-1) for 24 h and the resultant supernatants were filtered. The aqueous extract was freeze-dried while the chloroform extract was evaporated to dryness under reduce pressure to obtained the crude dried extracts, which were prepared in the concentration of 25, 50 and 100 mg mL 1 prior to the antibacterial assay. The results obtained demonstrated the potential of A. bilimbi extracts against some of the Gram-positive and-negative bacteria. The 100 mg mL 1 aqueous extract of A. bilimbi’s leaves and fruits showed positive antibacterial activity against the Gram-positive S. aureus, S. epidermis B. cereus, C. diphteriae with the latter also gave positive effect against K. rhizophila. Both extracts also exhibited positive antibacterial activity against the Gram-negative S. typhi, C. fuendii and A. hydrophila. On the other hand, the 100 mg mL 1 chloroform extracts of A. bilimbi’s leaves and fruits demonstrated antibacterial activity against the Gram-positive S. aureus, S. epidermis, B. cereus, K. rhizophila and C. diphteriae and Gram-negative S. typhi, C. fuendii, A. hydrophila and P. vulgaris. In conclusion, the A. bilimbi leaves and fruits possess potential antibacterial activity that requires thorough study.
format	Article
author	Zakaria, Zainul Amiruddin H, Zaiton Henie, E. F. P. Mat Jais, Abdul Manan E. Zainudin, Engku Nuraishah Huda
author_facet	Zakaria, Zainul Amiruddin H, Zaiton Henie, E. F. P. Mat Jais, Abdul Manan E. Zainudin, Engku Nuraishah Huda
author_sort	Zakaria, Zainul Amiruddin
title	In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
title_short	In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
title_full	In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
title_fullStr	In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
title_full_unstemmed	In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts
title_sort	in vitro antibacterial activity of averrhoa bilimbi l. leaves and fruits extracts
publisher	Medwell Journals
publishDate	2007
url	http://irep.iium.edu.my/55077/ http://irep.iium.edu.my/55077/ http://irep.iium.edu.my/55077/1/maxwell%20paper.pdf
first_indexed	2023-09-18T21:17:52Z
last_indexed	2023-09-18T21:17:52Z
_version_	1777411683828891648

In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts

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