Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea

This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic so...

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Main Authors:	Elgharbawy, Amal A.M., Alam, Md Zahangir, Mohd. Salleh, Hamzah
Format:	Article
Language:	English English
Published:	Asian Research Publishing Network 2016
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/55040/ http://irep.iium.edu.my/55040/ http://irep.iium.edu.my/55040/1/IPCER%20Amal%20Lipase.pdf http://irep.iium.edu.my/55040/7/55040_Characterization%20of%20low-cost%20lipase_scopus.pdf

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recordtype	eprints
spelling	iium-550402019-04-17T02:05:01Z http://irep.iium.edu.my/55040/ Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea Elgharbawy, Amal A.M. Alam, Md Zahangir Mohd. Salleh, Hamzah TP155 Chemical engineering This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.08.0 respectively.Zn 2+,Mg 2+ and Ca 2+ stimulated the enzyme while Fe 3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity.Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol,ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production. Asian Research Publishing Network 2016-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55040/1/IPCER%20Amal%20Lipase.pdf application/pdf en http://irep.iium.edu.my/55040/7/55040_Characterization%20of%20low-cost%20lipase_scopus.pdf Elgharbawy, Amal A.M. and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2016) Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea. ARPN Journal of Engineering and Applied Sciences, 11 (1). pp. 319-326. ISSN 1819-6608 http://www.arpnjournals.org/jeas/volume_01_2016.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Elgharbawy, Amal A.M. Alam, Md Zahangir Mohd. Salleh, Hamzah Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
description	This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.08.0 respectively.Zn 2+,Mg 2+ and Ca 2+ stimulated the enzyme while Fe 3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity.Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol,ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production.
format	Article
author	Elgharbawy, Amal A.M. Alam, Md Zahangir Mohd. Salleh, Hamzah
author_facet	Elgharbawy, Amal A.M. Alam, Md Zahangir Mohd. Salleh, Hamzah
author_sort	Elgharbawy, Amal A.M.
title	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
title_short	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
title_full	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
title_fullStr	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
title_full_unstemmed	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
title_sort	characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
publisher	Asian Research Publishing Network
publishDate	2016
url	http://irep.iium.edu.my/55040/ http://irep.iium.edu.my/55040/ http://irep.iium.edu.my/55040/1/IPCER%20Amal%20Lipase.pdf http://irep.iium.edu.my/55040/7/55040_Characterization%20of%20low-cost%20lipase_scopus.pdf
first_indexed	2023-09-18T21:17:48Z
last_indexed	2023-09-18T21:17:48Z
_version_	1777411680034095104

Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea

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