Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl

Entada spiralis Ridl. which is locally known as Sintok or Beluru is a woody climber with a characteristic spiral stem. The stem bark is traditionally used as natural shampoo and body wash among Malays due to its high content of saponins and its medicinal properties. The methanol extract of the stem...

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Main Authors:	Mohd Hassan, Norazian, Mat So'ad, Siti Zaiton, Harun, Aiza, Wan Mat Khalil, Wan Khaima Azira, Che Mohd Ramli, Neni Kartini
Format:	Conference or Workshop Item
Language:	English English
Published:	2010
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/5503/ http://irep.iium.edu.my/5503/ http://irep.iium.edu.my/5503/2/POSTER.pdf http://irep.iium.edu.my/5503/5/icast2010azian.pdf

id	iium-5503
recordtype	eprints
spelling	iium-55032014-04-15T20:07:59Z http://irep.iium.edu.my/5503/ Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl Mohd Hassan, Norazian Mat So'ad, Siti Zaiton Harun, Aiza Wan Mat Khalil, Wan Khaima Azira Che Mohd Ramli, Neni Kartini RS Pharmacy and materia medica Entada spiralis Ridl. which is locally known as Sintok or Beluru is a woody climber with a characteristic spiral stem. The stem bark is traditionally used as natural shampoo and body wash among Malays due to its high content of saponins and its medicinal properties. The methanol extract of the stem bark possess promising antimicrobial activity against some species of skin diseases caused microbes. A bioautographic profile as standard reference for qualitative analysis on the antimicrobial properties of E. spiralis stem barks was developed with the aim at validating the authenticity of the herbal material and quality control of its herbal preparations. A method of a combination of bioautographic assay and thin layer chromatography (TLC) analysis was employed to detect the antimicrobial biomarkers and to obtain the TLC profile, respectively. The methanol extract from the stem barks was fractionated through vacuum liquid chromatography to afford several fractions. Each fraction was assayed by Agar Disc Diffusion method and the active fractions were subjected to TLC analysis on silica gel plate. Five antimicrobial biomarkers were detected from the fractions by using bioautographic agar overlay assay against selected skin diseases caused bacterial and fungal strains, namely S. aureus, S. epidermidis, M. gypseum, T.mentagrophytes and T. tonsurans. The fraction which is eluted with methanol and chloroform at a ratio of 9:1 provides the best profile as standard reference for the efficacy analysis of E. spiralis as antimicrobial agent against skin infectious diseases microbes. 2010-11-26 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/5503/2/POSTER.pdf application/pdf en http://irep.iium.edu.my/5503/5/icast2010azian.pdf Mohd Hassan, Norazian and Mat So'ad, Siti Zaiton and Harun, Aiza and Wan Mat Khalil, Wan Khaima Azira and Che Mohd Ramli, Neni Kartini (2010) Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia. http://www.iium.edu.my/icast/2010
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Hassan, Norazian Mat So'ad, Siti Zaiton Harun, Aiza Wan Mat Khalil, Wan Khaima Azira Che Mohd Ramli, Neni Kartini Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
description	Entada spiralis Ridl. which is locally known as Sintok or Beluru is a woody climber with a characteristic spiral stem. The stem bark is traditionally used as natural shampoo and body wash among Malays due to its high content of saponins and its medicinal properties. The methanol extract of the stem bark possess promising antimicrobial activity against some species of skin diseases caused microbes. A bioautographic profile as standard reference for qualitative analysis on the antimicrobial properties of E. spiralis stem barks was developed with the aim at validating the authenticity of the herbal material and quality control of its herbal preparations. A method of a combination of bioautographic assay and thin layer chromatography (TLC) analysis was employed to detect the antimicrobial biomarkers and to obtain the TLC profile, respectively. The methanol extract from the stem barks was fractionated through vacuum liquid chromatography to afford several fractions. Each fraction was assayed by Agar Disc Diffusion method and the active fractions were subjected to TLC analysis on silica gel plate. Five antimicrobial biomarkers were detected from the fractions by using bioautographic agar overlay assay against selected skin diseases caused bacterial and fungal strains, namely S. aureus, S. epidermidis, M. gypseum, T.mentagrophytes and T. tonsurans. The fraction which is eluted with methanol and chloroform at a ratio of 9:1 provides the best profile as standard reference for the efficacy analysis of E. spiralis as antimicrobial agent against skin infectious diseases microbes.
format	Conference or Workshop Item
author	Mohd Hassan, Norazian Mat So'ad, Siti Zaiton Harun, Aiza Wan Mat Khalil, Wan Khaima Azira Che Mohd Ramli, Neni Kartini
author_facet	Mohd Hassan, Norazian Mat So'ad, Siti Zaiton Harun, Aiza Wan Mat Khalil, Wan Khaima Azira Che Mohd Ramli, Neni Kartini
author_sort	Mohd Hassan, Norazian
title	Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
title_short	Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
title_full	Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
title_fullStr	Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
title_full_unstemmed	Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl
title_sort	bioautographic profile of antimicrobial active extract from the stem barks of entada spiralis ridl
publishDate	2010
url	http://irep.iium.edu.my/5503/ http://irep.iium.edu.my/5503/ http://irep.iium.edu.my/5503/2/POSTER.pdf http://irep.iium.edu.my/5503/5/icast2010azian.pdf
first_indexed	2023-09-18T20:14:08Z
last_indexed	2023-09-18T20:14:08Z
_version_	1777407673980944384

Bioautographic profile of antimicrobial active extract from the stem barks of Entada spiralis Ridl

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