Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition

Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition

Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells...

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Main Authors: Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa’zamuddin, Hashim, Rosyafirah, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format: Conference or Workshop Item
Language:English
Published: UTM 2016
Subjects:
TA164 Bioengineering
Online Access:http://irep.iium.edu.my/54992/
http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf
id iium-54992
recordtype eprints
spelling iium-549922017-03-06T00:41:27Z http://irep.iium.edu.my/54992/ Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Zulkifly, Ahmad Hafiz Sha'ban, Munirah TA164 Bioengineering Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells in vivo. Incorporation of natural polymers i.e. atelocollagen and/or fibrin onto PLGA may compensate those effects. This present study aimed to evaluate the swelling capacity and degradation behaviour of PLGA when added with atelocollagen, fibrin or, combination of both atelocollagen and fibrin. Atelocollagen type II has no telopeptide region and forms gel like structure at body temperature. Plasma-derived fibrin is reported to reduce inflammation caused by PLGA. Combination of both atelocollagen and fibrin could enhance PLGA properties. Porous PLGA scaffolds were fabricated via solvent-casting/salt-leaching method using salt as porogen. Atelocollagen was then added and cross-linked with the PLGA using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) to form PLGA/atelocollagen (PA) scaffold. Plasma-derived fibrin was added to the PLGA and the prefabricated PLGA/atelocollagen scaffold to form PLGA/fibrin (PF) and PLGA/atelocollagen/fibrin scaffold (PAF) respectively. Calcium chloride was used to polymerize plasma-derived fibrin onto those scaffolds. Swelling test was performed by immersing the scaffolds in water for 24-hour. Hydrolytic degradation was performed by immersing the scaffolds in simulated body fluid (SBF) and the percentage of reduction was recorded at 28 days. Four scaffolds groups i.e. (1) PLGA alone, (2) PLGA/atelocollagen [PA], (3) PLGA/fibrin [PF], and (4) PLGA/atelocollagen/fibrin [PAF] were compared in this study. The swelling ratio of PA scaffold group (192.57±41.96%) had significantly increased compared to other groups (PLGA= 40.19±4.89%; PF=52.00±23.48%; PAF=66.59±13.38%). In terms of degradation, PA group exhibited significant weight loss (497.11±76.65%) over other groups after 28 days in SBF. These findings suggest PLGA/atelocollagen without fibrin is sufficient to increase swelling capacity and offer higher degradation properties. UTM 2016-02-24 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa’zamuddin and Hashim, Rosyafirah and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2016) Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition. In: 4th International Science Postgraduate Conference 2016, 22-24 Feb 2016, Johor Bahru.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
description Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells in vivo. Incorporation of natural polymers i.e. atelocollagen and/or fibrin onto PLGA may compensate those effects. This present study aimed to evaluate the swelling capacity and degradation behaviour of PLGA when added with atelocollagen, fibrin or, combination of both atelocollagen and fibrin. Atelocollagen type II has no telopeptide region and forms gel like structure at body temperature. Plasma-derived fibrin is reported to reduce inflammation caused by PLGA. Combination of both atelocollagen and fibrin could enhance PLGA properties. Porous PLGA scaffolds were fabricated via solvent-casting/salt-leaching method using salt as porogen. Atelocollagen was then added and cross-linked with the PLGA using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) to form PLGA/atelocollagen (PA) scaffold. Plasma-derived fibrin was added to the PLGA and the prefabricated PLGA/atelocollagen scaffold to form PLGA/fibrin (PF) and PLGA/atelocollagen/fibrin scaffold (PAF) respectively. Calcium chloride was used to polymerize plasma-derived fibrin onto those scaffolds. Swelling test was performed by immersing the scaffolds in water for 24-hour. Hydrolytic degradation was performed by immersing the scaffolds in simulated body fluid (SBF) and the percentage of reduction was recorded at 28 days. Four scaffolds groups i.e. (1) PLGA alone, (2) PLGA/atelocollagen [PA], (3) PLGA/fibrin [PF], and (4) PLGA/atelocollagen/fibrin [PAF] were compared in this study. The swelling ratio of PA scaffold group (192.57±41.96%) had significantly increased compared to other groups (PLGA= 40.19±4.89%; PF=52.00±23.48%; PAF=66.59±13.38%). In terms of degradation, PA group exhibited significant weight loss (497.11±76.65%) over other groups after 28 days in SBF. These findings suggest PLGA/atelocollagen without fibrin is sufficient to increase swelling capacity and offer higher degradation properties.
format Conference or Workshop Item
author Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
author_facet Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Md Nazir, Noorhidayah
Ahmad Radzi, Muhammad Aa’zamuddin
Hashim, Rosyafirah
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
author_sort Mohamad, Mohd Yusof
title Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_short Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_full Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_fullStr Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_full_unstemmed Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_sort swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
publisher UTM
publishDate 2016
url http://irep.iium.edu.my/54992/
http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf
first_indexed 2023-09-18T21:17:44Z
last_indexed 2023-09-18T21:17:44Z
_version_ 1777411675793653760

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