In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration

In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration

Poly (lactic-co-glycolic acid) (PLGA) is a widely used synthetic biomaterial in tissue engineering. Incorporation of natural atelocollagen and fibrin may provide PLGA a better surface property for cellular adhesion and proliferation. This paper aims to evaluate PLGA/Atelocollagen/Fibrin hybrid scaff...

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Main Authors: Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format: Conference or Workshop Item
Language:English
Published: 2016
Subjects:
TA164 Bioengineering
Online Access:http://irep.iium.edu.my/54980/
http://irep.iium.edu.my/54980/1/Yusof%206thMTERMS2016%20Abstract.pdf
id iium-54980
recordtype eprints
spelling iium-549802017-02-13T06:08:08Z http://irep.iium.edu.my/54980/ In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah TA164 Bioengineering Poly (lactic-co-glycolic acid) (PLGA) is a widely used synthetic biomaterial in tissue engineering. Incorporation of natural atelocollagen and fibrin may provide PLGA a better surface property for cellular adhesion and proliferation. This paper aims to evaluate PLGA/Atelocollagen/Fibrin hybrid scaffolds seeded with IVD’s annulus fibrosus (AF) cells after in vivo implantation. Porous 3D PLGA were fabricated via solvent-casting and salt-leaching technique. PLGA was crosslinked with atelocollagen (PA). Fibrin was pipetted onto PLGA (PF) and PA (PAF) scaffolds. Rabbits’ AF cells were seeded onto each scaffold groups i.e. PLGA only, PA, PF and PAF. The “cells-scaffolds” constructs were cultured for three-weeks and implanted subcutaneously in athymic nude mice for four-week. All constructs were evaluated for gross morphology, histology, immunohistochemistry (IHC) against collagen I and II with PLGA construct as control group. Sulphated glycosaminoglycan (sGAG) and DNA contents were compared to that of monolayer AF culture. All groups exhibited significant reduction in size but showed increment in weight. Histology analysis indicated high cellular density, supported by high DNA content in all groups. The IHC showed presence of collagen I and II. The PAF showed a significantly higher sGAG content than the other groups. Degradation of PLGA did not hinder tissue formation. Atelocollagen and fibrin may contribute to a higher cellular proliferation and extracellular matrix production in PLGA. Further evaluation using gene expression shall be performed to study the intrinsic properties and possible mechanisms involved. PLGA/Atelocollagen/Fibrin hybrid scaffolds may become potential candidate for tissue engineering application. 2016-11-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54980/1/Yusof%206thMTERMS2016%20Abstract.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2016) In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17-18 Nov 2016, Seberang Jaya, Pulau Pinang.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
description Poly (lactic-co-glycolic acid) (PLGA) is a widely used synthetic biomaterial in tissue engineering. Incorporation of natural atelocollagen and fibrin may provide PLGA a better surface property for cellular adhesion and proliferation. This paper aims to evaluate PLGA/Atelocollagen/Fibrin hybrid scaffolds seeded with IVD’s annulus fibrosus (AF) cells after in vivo implantation. Porous 3D PLGA were fabricated via solvent-casting and salt-leaching technique. PLGA was crosslinked with atelocollagen (PA). Fibrin was pipetted onto PLGA (PF) and PA (PAF) scaffolds. Rabbits’ AF cells were seeded onto each scaffold groups i.e. PLGA only, PA, PF and PAF. The “cells-scaffolds” constructs were cultured for three-weeks and implanted subcutaneously in athymic nude mice for four-week. All constructs were evaluated for gross morphology, histology, immunohistochemistry (IHC) against collagen I and II with PLGA construct as control group. Sulphated glycosaminoglycan (sGAG) and DNA contents were compared to that of monolayer AF culture. All groups exhibited significant reduction in size but showed increment in weight. Histology analysis indicated high cellular density, supported by high DNA content in all groups. The IHC showed presence of collagen I and II. The PAF showed a significantly higher sGAG content than the other groups. Degradation of PLGA did not hinder tissue formation. Atelocollagen and fibrin may contribute to a higher cellular proliferation and extracellular matrix production in PLGA. Further evaluation using gene expression shall be performed to study the intrinsic properties and possible mechanisms involved. PLGA/Atelocollagen/Fibrin hybrid scaffolds may become potential candidate for tissue engineering application.
format Conference or Workshop Item
author Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
author_facet Mohamad, Mohd Yusof
Mohamed Amin, Muhammad Azri Ifwat
Zulkifly, Ahmad Hafiz
Sha'ban, Munirah
author_sort Mohamad, Mohd Yusof
title In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
title_short In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
title_full In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
title_fullStr In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
title_full_unstemmed In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
title_sort in vivo evaluation of 3-dimensional plga/ atelocollagen/ fibrin scaffolds for intervertebral disc (ivd) regeneration
publishDate 2016
url http://irep.iium.edu.my/54980/
http://irep.iium.edu.my/54980/1/Yusof%206thMTERMS2016%20Abstract.pdf
first_indexed 2023-09-18T21:17:44Z
last_indexed 2023-09-18T21:17:44Z
_version_ 1777411674919141376

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