Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen

Purpose: Cisplatin and 5-Fluorouracil are the critical components of therapeutic regimen in a broad range of malignancies. Their therapeutic efficacy is limited due to the development of toxic side effects and drug resistance by the cancer cells. Cisplatin toxic side effects are usually associated w...

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Main Authors:	Khaing, Ei Phyo, Tun, Aung Win, Lertrit, Patcharee, Suthammarak, Wichit, Kaewsutth, Supannee, Kyaw, May Pyone
Format:	Article
Language:	English
Published:	Institut Perubatan dan Pergigian Termaju (IPPT),Universiti Sains Malaysia 2016
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/54969/ http://irep.iium.edu.my/54969/ http://irep.iium.edu.my/54969/12/54969_Mitochondrial%20DNA.pdf

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recordtype	eprints
spelling	iium-549692017-10-17T00:55:29Z http://irep.iium.edu.my/54969/ Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen Khaing, Ei Phyo Tun, Aung Win Lertrit, Patcharee Suthammarak, Wichit Kaewsutth, Supannee Kyaw, May Pyone RM Therapeutics. Pharmacology RM300 Drugs and their action Purpose: Cisplatin and 5-Fluorouracil are the critical components of therapeutic regimen in a broad range of malignancies. Their therapeutic efficacy is limited due to the development of toxic side effects and drug resistance by the cancer cells. Cisplatin toxic side effects are usually associated with mitochondrial injury in vivo and in vitro. In vitro evidence indicates that cisplatin decreases mitochondrial DNA (mtDNA) copy number. There is limited information on the effect of cisplatin on human mtDNA quantity. In order to clarify the effects of cisplatin and 5-FU on mtDNA in human, this study focused on the changes of mtDNA quantitation in cancer patients who were treated with cisplatin-based regimens. Methods: 24 blood samples from head and neck cancer patients were collected before chemotherapy and after second cycle of chemotherapy. Total DNA was extracted from peripheral blood leukocytes by salting out method. Validation of primer pairs were done by conventional PCR and checked by gel electrophoresis. The relative changes of mtDNA quantity were determined by quantitative PCR targeting Cyt-B genes normalizing with the nuclear gene PARL. Result: Comparing 24 samples, there was an increased mtDNA level after second cycle of chemotherapy, though it did Wilcoxon signed ranked test). However, 58% of the total patients had relatively increased mtDNA amount while 42% of the total patients showed decreased mtDNA amount after second cycle of chemotherapy (P<0.05, paired t-test). Conclusion: Overall, Cisplatin-based chemotherapy can affect mtDNA quantity changes in leucocytes of head and neck cancer patients. Institut Perubatan dan Pergigian Termaju (IPPT),Universiti Sains Malaysia 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54969/12/54969_Mitochondrial%20DNA.pdf Khaing, Ei Phyo and Tun, Aung Win and Lertrit, Patcharee and Suthammarak, Wichit and Kaewsutth, Supannee and Kyaw, May Pyone (2016) Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen. Journal of Biomedical and Clinical Sciences (JBCS), 1 (1 (Supplement issue)). p. 8. E-ISSN 2550-147X http://apps.amdi.usm.my/journal/index.php/jbcs/issue/view/Vol%201%2C%20No%201%2C%20Dec%202016%20-%20Special%20Issue
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action Khaing, Ei Phyo Tun, Aung Win Lertrit, Patcharee Suthammarak, Wichit Kaewsutth, Supannee Kyaw, May Pyone Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
description	Purpose: Cisplatin and 5-Fluorouracil are the critical components of therapeutic regimen in a broad range of malignancies. Their therapeutic efficacy is limited due to the development of toxic side effects and drug resistance by the cancer cells. Cisplatin toxic side effects are usually associated with mitochondrial injury in vivo and in vitro. In vitro evidence indicates that cisplatin decreases mitochondrial DNA (mtDNA) copy number. There is limited information on the effect of cisplatin on human mtDNA quantity. In order to clarify the effects of cisplatin and 5-FU on mtDNA in human, this study focused on the changes of mtDNA quantitation in cancer patients who were treated with cisplatin-based regimens. Methods: 24 blood samples from head and neck cancer patients were collected before chemotherapy and after second cycle of chemotherapy. Total DNA was extracted from peripheral blood leukocytes by salting out method. Validation of primer pairs were done by conventional PCR and checked by gel electrophoresis. The relative changes of mtDNA quantity were determined by quantitative PCR targeting Cyt-B genes normalizing with the nuclear gene PARL. Result: Comparing 24 samples, there was an increased mtDNA level after second cycle of chemotherapy, though it did Wilcoxon signed ranked test). However, 58% of the total patients had relatively increased mtDNA amount while 42% of the total patients showed decreased mtDNA amount after second cycle of chemotherapy (P<0.05, paired t-test). Conclusion: Overall, Cisplatin-based chemotherapy can affect mtDNA quantity changes in leucocytes of head and neck cancer patients.
format	Article
author	Khaing, Ei Phyo Tun, Aung Win Lertrit, Patcharee Suthammarak, Wichit Kaewsutth, Supannee Kyaw, May Pyone
author_facet	Khaing, Ei Phyo Tun, Aung Win Lertrit, Patcharee Suthammarak, Wichit Kaewsutth, Supannee Kyaw, May Pyone
author_sort	Khaing, Ei Phyo
title	Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
title_short	Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
title_full	Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
title_fullStr	Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
title_full_unstemmed	Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
title_sort	mitochondrial dna quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen
publisher	Institut Perubatan dan Pergigian Termaju (IPPT),Universiti Sains Malaysia
publishDate	2016
url	http://irep.iium.edu.my/54969/ http://irep.iium.edu.my/54969/ http://irep.iium.edu.my/54969/12/54969_Mitochondrial%20DNA.pdf
first_indexed	2023-09-18T21:17:43Z
last_indexed	2023-09-18T21:17:43Z
_version_	1777411674492370944

Mitochondrial DNA quantity changes in leucocytes of cancer patients on cisplatin and 5-fluorouracil regimen

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