Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo

Introduction: This study was conducted to evaluate the phytochemical contents and antimalarial properties of the essential oils extracted from the leaves of Malaysian Plectranthus amboinicus in mice infected with Plasmodium berghei. Methods: The essential oils were extracted and prepared by using...

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Main Authors:	Ramli, Norazsida, Syed Ahmad, Pakeer Oothuman, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English English
Published:	IMJM 2016
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/2/MRS2016-ABSTRACT-BOOK-58.pdf http://irep.iium.edu.my/54964/8/54964_Evaluation%20of%20the%20phytochemical.pdf

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spelling	iium-549642018-02-26T10:44:18Z http://irep.iium.edu.my/54964/ Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo Ramli, Norazsida Syed Ahmad, Pakeer Oothuman Bakhtiar, M. Taher R Medicine (General) Introduction: This study was conducted to evaluate the phytochemical contents and antimalarial properties of the essential oils extracted from the leaves of Malaysian Plectranthus amboinicus in mice infected with Plasmodium berghei. Methods: The essential oils were extracted and prepared by using a steam distillation technique and subjected to phytochemical screening by using gas chromatography–mass spectrometry (GC-MS). The antimalarial activity of different extract doses of the essential oil was tested in vivo in ICR (Instritute of Cancer Research) mice infected with Plasmodium berghei (PZZ1/100) during early, established and residual infections. The control groups were treated with distilled water (containing 10% DMSO, the solvent of the test extracts) and 2 standard drugs: chloroquine and Fansidar. Results: In all, 5 compounds made up 88.34% of total oil and the major chemical compounds were carvacrol (85.14%), thymoquinone (1.65%), terpinen-4-ol (0.70%), octenol (0.62%) and thymol (0.23%). Antimalarial assay showed this essential oil as a potential prophylactic agent with the percentage chemosuppression of 45.23%, 18.28%, 45.38% and 58.26%, while treated with 50, 200, 400 and 1000 µL/kg respectively of essential oil. It also showed a potential as a curative agent with percentage of chemosuppression of 54.10%, 47.35%, 56.75% and 65.38% while treated with the above dose of essential oil. Statistically, no reduction of parasitemia was calculated for suppressive test. Conclusions: The extract has prophylactic and curative effects on P.berghei in mice. IMJM 2016-08-03 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54964/2/MRS2016-ABSTRACT-BOOK-58.pdf application/pdf en http://irep.iium.edu.my/54964/8/54964_Evaluation%20of%20the%20phytochemical.pdf Ramli, Norazsida and Syed Ahmad, Pakeer Oothuman and Bakhtiar, M. Taher (2016) Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-58.pdf 90
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Ramli, Norazsida Syed Ahmad, Pakeer Oothuman Bakhtiar, M. Taher Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
description	Introduction: This study was conducted to evaluate the phytochemical contents and antimalarial properties of the essential oils extracted from the leaves of Malaysian Plectranthus amboinicus in mice infected with Plasmodium berghei. Methods: The essential oils were extracted and prepared by using a steam distillation technique and subjected to phytochemical screening by using gas chromatography–mass spectrometry (GC-MS). The antimalarial activity of different extract doses of the essential oil was tested in vivo in ICR (Instritute of Cancer Research) mice infected with Plasmodium berghei (PZZ1/100) during early, established and residual infections. The control groups were treated with distilled water (containing 10% DMSO, the solvent of the test extracts) and 2 standard drugs: chloroquine and Fansidar. Results: In all, 5 compounds made up 88.34% of total oil and the major chemical compounds were carvacrol (85.14%), thymoquinone (1.65%), terpinen-4-ol (0.70%), octenol (0.62%) and thymol (0.23%). Antimalarial assay showed this essential oil as a potential prophylactic agent with the percentage chemosuppression of 45.23%, 18.28%, 45.38% and 58.26%, while treated with 50, 200, 400 and 1000 µL/kg respectively of essential oil. It also showed a potential as a curative agent with percentage of chemosuppression of 54.10%, 47.35%, 56.75% and 65.38% while treated with the above dose of essential oil. Statistically, no reduction of parasitemia was calculated for suppressive test. Conclusions: The extract has prophylactic and curative effects on P.berghei in mice.
format	Conference or Workshop Item
author	Ramli, Norazsida Syed Ahmad, Pakeer Oothuman Bakhtiar, M. Taher
author_facet	Ramli, Norazsida Syed Ahmad, Pakeer Oothuman Bakhtiar, M. Taher
author_sort	Ramli, Norazsida
title	Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
title_short	Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
title_full	Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
title_fullStr	Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
title_full_unstemmed	Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
title_sort	evaluation of the phytochemical content and the use of the essential oil from the leaves of malaysian plectranthus amboinicus (lour) spreng as antimalarial in vivo
publisher	IMJM
publishDate	2016
url	http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/2/MRS2016-ABSTRACT-BOOK-58.pdf http://irep.iium.edu.my/54964/8/54964_Evaluation%20of%20the%20phytochemical.pdf
first_indexed	2023-09-18T21:17:43Z
last_indexed	2023-09-18T21:17:43Z
_version_	1777411674214498304

Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo

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