Evaluation of the phytochemical content and the use of the essential oil from the leaves of Malaysian Plectranthus amboinicus (lour) spreng as antimalarial in vivo
Introduction: This study was conducted to evaluate the phytochemical contents and antimalarial properties of the essential oils extracted from the leaves of Malaysian Plectranthus amboinicus in mice infected with Plasmodium berghei. Methods: The essential oils were extracted and prepared by using...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
IMJM
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/ http://irep.iium.edu.my/54964/2/MRS2016-ABSTRACT-BOOK-58.pdf http://irep.iium.edu.my/54964/8/54964_Evaluation%20of%20the%20phytochemical.pdf |
| Summary: | Introduction: This study was conducted to evaluate the phytochemical contents and
antimalarial properties of the essential oils extracted from the leaves of
Malaysian Plectranthus amboinicus in mice infected with Plasmodium berghei.
Methods: The essential oils were extracted and prepared by using a steam distillation
technique and subjected to phytochemical screening by using gas chromatography–mass
spectrometry (GC-MS). The antimalarial activity of different extract doses of the
essential oil was tested in vivo in ICR (Instritute of Cancer Research) mice infected
with Plasmodium berghei (PZZ1/100) during early, established and residual infections. The
control groups were treated with distilled water (containing 10% DMSO, the solvent of the
test extracts) and 2 standard drugs: chloroquine and Fansidar. Results: In all, 5
compounds made up 88.34% of total oil and the major chemical compounds were carvacrol
(85.14%), thymoquinone (1.65%), terpinen-4-ol (0.70%), octenol (0.62%) and thymol
(0.23%). Antimalarial assay showed this essential oil as a potential prophylactic agent with
the percentage chemosuppression of 45.23%, 18.28%, 45.38% and 58.26%, while treated
with 50, 200, 400 and 1000 µL/kg respectively of essential oil. It also showed a potential
as a curative agent with percentage of chemosuppression of 54.10%, 47.35%, 56.75% and
65.38% while treated with the above dose of essential oil. Statistically, no reduction of
parasitemia was calculated for suppressive test. Conclusions: The extract has prophylactic
and curative effects on P.berghei in mice. |
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