Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line

Arborinine is an acridone alkaloid found exclusively in Rutacae family has been shown to inhibit proliferation of a wide variety of cancer cell lines. The present study is aimed to isolate and investigate the anti-proliferative activity of arborinine, isolated from a Malaysian Rutaceae, G. pentapyll...

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Main Authors:	Mohd Hussain, Nurlaili Najmie, Uzir, Bisha Fathamah, Mohd Hassan, Norazian
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/54885/ http://irep.iium.edu.my/54885/29/54885.pdf http://irep.iium.edu.my/54885/30/54885-certificate-1.pdf

id	iium-54885
recordtype	eprints
spelling	iium-548852017-02-21T02:01:40Z http://irep.iium.edu.my/54885/ Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line Mohd Hussain, Nurlaili Najmie Uzir, Bisha Fathamah Mohd Hassan, Norazian RM Therapeutics. Pharmacology RM300 Drugs and their action Arborinine is an acridone alkaloid found exclusively in Rutacae family has been shown to inhibit proliferation of a wide variety of cancer cell lines. The present study is aimed to isolate and investigate the anti-proliferative activity of arborinine, isolated from a Malaysian Rutaceae, G. pentapylla (Retz. D) on human mammary gland adenocarcinoma, MCF-7 cell line. Isolation and characterization of arborinine were conducted from the leaves of G.pentaphylla collected from Kelantan via acid-base extraction and wet column chromatographic techniques. The structure of arborinine was elucidated by comparing its spectroscopic data with published reports. Arborinine was studied for its anti-proliferative activity with the help of MTT assay and further with TUNEL and caspase-3/7 assay to investigate the apoptosis mechanism. The result showed that 0.1295g (12.15%) of arborinine was yield from 1.0653g of alkaloidal extract. Arborinine inhibited the proliferation of MCF-7 in a dose- and time-dependent manner with IC50 (6.25± 1.38µM). Dark brown apoptotic nuclei were observed as a result of DNA fragmentation via TUNEL assay further confirmed the mode of cell death via apoptosis. Activation of caspase-3/7 may suggest the apoptosis mechanism induced through the mitochondrial pathway on MCF-7 cells. The data presented suggest that arborinine can be a potential structure for further investigation in the development of a new anticancer agent. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/54885/29/54885.pdf application/pdf en http://irep.iium.edu.my/54885/30/54885-certificate-1.pdf Mohd Hussain, Nurlaili Najmie and Uzir, Bisha Fathamah and Mohd Hassan, Norazian (2016) Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line. In: 2nd ICIP (International Conference on Industrial Pharmacy) 2016, 15-16th August 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action Mohd Hussain, Nurlaili Najmie Uzir, Bisha Fathamah Mohd Hassan, Norazian Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
description	Arborinine is an acridone alkaloid found exclusively in Rutacae family has been shown to inhibit proliferation of a wide variety of cancer cell lines. The present study is aimed to isolate and investigate the anti-proliferative activity of arborinine, isolated from a Malaysian Rutaceae, G. pentapylla (Retz. D) on human mammary gland adenocarcinoma, MCF-7 cell line. Isolation and characterization of arborinine were conducted from the leaves of G.pentaphylla collected from Kelantan via acid-base extraction and wet column chromatographic techniques. The structure of arborinine was elucidated by comparing its spectroscopic data with published reports. Arborinine was studied for its anti-proliferative activity with the help of MTT assay and further with TUNEL and caspase-3/7 assay to investigate the apoptosis mechanism. The result showed that 0.1295g (12.15%) of arborinine was yield from 1.0653g of alkaloidal extract. Arborinine inhibited the proliferation of MCF-7 in a dose- and time-dependent manner with IC50 (6.25± 1.38µM). Dark brown apoptotic nuclei were observed as a result of DNA fragmentation via TUNEL assay further confirmed the mode of cell death via apoptosis. Activation of caspase-3/7 may suggest the apoptosis mechanism induced through the mitochondrial pathway on MCF-7 cells. The data presented suggest that arborinine can be a potential structure for further investigation in the development of a new anticancer agent.
format	Conference or Workshop Item
author	Mohd Hussain, Nurlaili Najmie Uzir, Bisha Fathamah Mohd Hassan, Norazian
author_facet	Mohd Hussain, Nurlaili Najmie Uzir, Bisha Fathamah Mohd Hassan, Norazian
author_sort	Mohd Hussain, Nurlaili Najmie
title	Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
title_short	Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
title_full	Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
title_fullStr	Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
title_full_unstemmed	Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line
title_sort	arborinine from glycosmis pentaphylla (retz.) dc induces apoptosis through activation of caspase-3/7 in human mammary gland adenocarcinoma (mcf-7) cell line
publishDate	2016
url	http://irep.iium.edu.my/54885/ http://irep.iium.edu.my/54885/29/54885.pdf http://irep.iium.edu.my/54885/30/54885-certificate-1.pdf
first_indexed	2023-09-18T21:17:36Z
last_indexed	2023-09-18T21:17:36Z
_version_	1777411666721374208

Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line

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