Flooding impact on distribution of pathogenic bacteria in Pahang River

Pahang River is one of the important inland aquatic biodiversity resources that provides major economic activities along the river. It is an excellent source of food and habitat for organisms including microorganisms leading to higher bacterial diversity. This analytical study determines the impact...

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Main Authors:	Abd Razak, Fatin Hafawati, Hadry Nordin, Noor Faizul Hadry, Khan Chowdhury, Ahmed Jalal, Yunus, Kamaruzzaman
Format:	Article
Language:	English
Published:	Kulliyyah of Medicine, IIUM, Kuantan 2016
Subjects:	SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/54774/ http://irep.iium.edu.my/54774/ http://irep.iium.edu.my/54774/1/5.%20Flooding%20impact.pdf

id	iium-54774
recordtype	eprints
spelling	iium-547742017-12-06T05:04:48Z http://irep.iium.edu.my/54774/ Flooding impact on distribution of pathogenic bacteria in Pahang River Abd Razak, Fatin Hafawati Hadry Nordin, Noor Faizul Hadry Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman SH Aquaculture. Fisheries. Angling Pahang River is one of the important inland aquatic biodiversity resources that provides major economic activities along the river. It is an excellent source of food and habitat for organisms including microorganisms leading to higher bacterial diversity. This analytical study determines the impact of flooding event to the bacterial distribution and pathogenic bacteria in the river. Methods: Sampling was done during pre-monsoon and post monsoon seasons. Water samples were taken at 19 stations along the Pahang River. Bacterial was isolated from the water samples using cultivation method. Subsequently DNA from the isolated bacteria was extracted using conventional method. 16s RNA was used in the study to amplify the bacterial DNA. Following restriction fragment length polymorphism (RFLP), selected bacteria were sent for sequencing to the First Base company. The sequences were then analyzed using blast from NCBI to obtain the bacterial name. Results: Here, we evaluated the colony forming unit (CFU) and the presence of potentially pathogenic bacteria in the river before and after the flood event in 2014. The CFU calculated was lower before the flooding event and Bacillus sp. were found in many of the water samples. After the flooding event pathogenic bacteria such as Klebsiella pneumoniae, Serratia marcescens and Enterobacter cloacae were discovered while the CFU calculated was shown to be higher. Conclusions: The pathogenic bacteria diversity was increased after the flood in 2014 in Pahang River. The river became contaminated with various types of potential human bacterial pathogens which might pose a health risk to the public. Kulliyyah of Medicine, IIUM, Kuantan 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54774/1/5.%20Flooding%20impact.pdf Abd Razak, Fatin Hafawati and Hadry Nordin, Noor Faizul Hadry and Khan Chowdhury, Ahmed Jalal and Yunus, Kamaruzzaman (2016) Flooding impact on distribution of pathogenic bacteria in Pahang River. The International Medical Journal of Malaysia, 15 (Supplement Issue). p. 11. http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-11.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling
spellingShingle	SH Aquaculture. Fisheries. Angling Abd Razak, Fatin Hafawati Hadry Nordin, Noor Faizul Hadry Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Flooding impact on distribution of pathogenic bacteria in Pahang River
description	Pahang River is one of the important inland aquatic biodiversity resources that provides major economic activities along the river. It is an excellent source of food and habitat for organisms including microorganisms leading to higher bacterial diversity. This analytical study determines the impact of flooding event to the bacterial distribution and pathogenic bacteria in the river. Methods: Sampling was done during pre-monsoon and post monsoon seasons. Water samples were taken at 19 stations along the Pahang River. Bacterial was isolated from the water samples using cultivation method. Subsequently DNA from the isolated bacteria was extracted using conventional method. 16s RNA was used in the study to amplify the bacterial DNA. Following restriction fragment length polymorphism (RFLP), selected bacteria were sent for sequencing to the First Base company. The sequences were then analyzed using blast from NCBI to obtain the bacterial name. Results: Here, we evaluated the colony forming unit (CFU) and the presence of potentially pathogenic bacteria in the river before and after the flood event in 2014. The CFU calculated was lower before the flooding event and Bacillus sp. were found in many of the water samples. After the flooding event pathogenic bacteria such as Klebsiella pneumoniae, Serratia marcescens and Enterobacter cloacae were discovered while the CFU calculated was shown to be higher. Conclusions: The pathogenic bacteria diversity was increased after the flood in 2014 in Pahang River. The river became contaminated with various types of potential human bacterial pathogens which might pose a health risk to the public.
format	Article
author	Abd Razak, Fatin Hafawati Hadry Nordin, Noor Faizul Hadry Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman
author_facet	Abd Razak, Fatin Hafawati Hadry Nordin, Noor Faizul Hadry Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman
author_sort	Abd Razak, Fatin Hafawati
title	Flooding impact on distribution of pathogenic bacteria in Pahang River
title_short	Flooding impact on distribution of pathogenic bacteria in Pahang River
title_full	Flooding impact on distribution of pathogenic bacteria in Pahang River
title_fullStr	Flooding impact on distribution of pathogenic bacteria in Pahang River
title_full_unstemmed	Flooding impact on distribution of pathogenic bacteria in Pahang River
title_sort	flooding impact on distribution of pathogenic bacteria in pahang river
publisher	Kulliyyah of Medicine, IIUM, Kuantan
publishDate	2016
url	http://irep.iium.edu.my/54774/ http://irep.iium.edu.my/54774/ http://irep.iium.edu.my/54774/1/5.%20Flooding%20impact.pdf
first_indexed	2023-09-18T21:17:27Z
last_indexed	2023-09-18T21:17:27Z
_version_	1777411658000367616

Flooding impact on distribution of pathogenic bacteria in Pahang River

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