Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro

Purpose: To evaluate the in vitro formation of 3D tissue engineered constructs (TECs) using rabbits’ annulus fibrosus (AF) cells seeded on poly(lactic-co-glycolic acid) (PLGA) based scaffolds. Methods: Porous disc-shaped PLGA was fabricated using solvent casting and salt leaching technique. It wa...

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Main Authors:	Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	IIUM 2016
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/54737/ http://irep.iium.edu.my/54737/1/Azri%206thMTERMS2016%20Abstract.pdf

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spelling	iium-547372017-02-12T15:50:52Z http://irep.iium.edu.my/54737/ Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah TA164 Bioengineering Purpose: To evaluate the in vitro formation of 3D tissue engineered constructs (TECs) using rabbits’ annulus fibrosus (AF) cells seeded on poly(lactic-co-glycolic acid) (PLGA) based scaffolds. Methods: Porous disc-shaped PLGA was fabricated using solvent casting and salt leaching technique. It was crosslinked with atelocollagen to form “PA”scaffold group. Fibrin was added to PLGA and PLGA-atelocollagen composite to form “PF” and “PAF” scaffolds, respectively. The AF cells were seeded into the prefabricated scaffolds (1.0x105 cells per scaffold) to form the following TECs groups: AF+PLGA (AFP; control), AF+PLGA+atelocollagen (AFPA), AF+PLGA+fibrin (AFPF) and AF+PLGA+atelocollagen+fibrin (AFPAF). The resulting TECs were cultured for three-week and evaluated for cells viability using MTT assay, cellular morphology and attachment using SEM, cartilaginous matrix production using sGAG assay and DNA content using PicoGreen® assay. Results: Significant number of viable cells was observed in the AFPAF group (987,985.7±286,858.9 cells)when compared to other TECs(AFP: 373,319.0±5,456.9; AFPA: 547,763.4±66,038.2; AFPF: 463,763.4±46,160.8 cells). Cellular morphology and attachment were comparable in all TECs. The AFPA has the highest sGAG accumulation (0.279±0.117mg/ml) but shows no statistical difference when compared to the other TECs (AFP: 0.083±0.038; AFPF: 0.237±0.131; AFPAF: 0.181±0.024 mg/ml).The AFPF has the highest DNA content (1,919.338±89.050 ng/ml) but shows no statistical difference when comparedto the other TECs (AFP: 485.659±27.468; AFPA: 845.987±82.134; AFPAF: 1,575.007±307.174 ng/ml). Hence, atelocollagen seemed to provide better environment for cellular attachment and proliferation. This unique collagenous material also promotes sGAG production and DNA content in TECs. Conclusion: The incorporation of atelocollagen into PLGA scaffold enhances the formation of TECs in vitro. IIUM 2016-11-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54737/1/Azri%206thMTERMS2016%20Abstract.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2016) Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17 - 18 Nov 2016, Pulang PiNang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
description	Purpose: To evaluate the in vitro formation of 3D tissue engineered constructs (TECs) using rabbits’ annulus fibrosus (AF) cells seeded on poly(lactic-co-glycolic acid) (PLGA) based scaffolds. Methods: Porous disc-shaped PLGA was fabricated using solvent casting and salt leaching technique. It was crosslinked with atelocollagen to form “PA”scaffold group. Fibrin was added to PLGA and PLGA-atelocollagen composite to form “PF” and “PAF” scaffolds, respectively. The AF cells were seeded into the prefabricated scaffolds (1.0x105 cells per scaffold) to form the following TECs groups: AF+PLGA (AFP; control), AF+PLGA+atelocollagen (AFPA), AF+PLGA+fibrin (AFPF) and AF+PLGA+atelocollagen+fibrin (AFPAF). The resulting TECs were cultured for three-week and evaluated for cells viability using MTT assay, cellular morphology and attachment using SEM, cartilaginous matrix production using sGAG assay and DNA content using PicoGreen® assay. Results: Significant number of viable cells was observed in the AFPAF group (987,985.7±286,858.9 cells)when compared to other TECs(AFP: 373,319.0±5,456.9; AFPA: 547,763.4±66,038.2; AFPF: 463,763.4±46,160.8 cells). Cellular morphology and attachment were comparable in all TECs. The AFPA has the highest sGAG accumulation (0.279±0.117mg/ml) but shows no statistical difference when compared to the other TECs (AFP: 0.083±0.038; AFPF: 0.237±0.131; AFPAF: 0.181±0.024 mg/ml).The AFPF has the highest DNA content (1,919.338±89.050 ng/ml) but shows no statistical difference when comparedto the other TECs (AFP: 485.659±27.468; AFPA: 845.987±82.134; AFPAF: 1,575.007±307.174 ng/ml). Hence, atelocollagen seemed to provide better environment for cellular attachment and proliferation. This unique collagenous material also promotes sGAG production and DNA content in TECs. Conclusion: The incorporation of atelocollagen into PLGA scaffold enhances the formation of TECs in vitro.
format	Conference or Workshop Item
author	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_facet	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_sort	Mohamad, Mohd Yusof
title	Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
title_short	Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
title_full	Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
title_fullStr	Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
title_full_unstemmed	Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
title_sort	poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
publisher	IIUM
publishDate	2016
url	http://irep.iium.edu.my/54737/ http://irep.iium.edu.my/54737/1/Azri%206thMTERMS2016%20Abstract.pdf
first_indexed	2023-09-18T21:17:24Z
last_indexed	2023-09-18T21:17:24Z
_version_	1777411654940622848

Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro

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