Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)

Introduction: Increasing antibiotic resistance in bacteria is a serious global problem. This study was aimed to extract the antibiotic Chloramphenicol (CAP) from different parts of the fish flesh. Methods: Fish fleshes were excised from two commercially important fishes: Oreochromatis niloticus.(...

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Main Authors:	Khan Chowdhury, Ahmed Jalal, Helaluddin, Abul Bashar Mohammed, John, Akbar, Bulbul, Mahbuba, M. N., Nur Hafizah
Format:	Article
Language:	English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/54493/ http://irep.iium.edu.my/54493/ http://irep.iium.edu.my/54493/1/65.pdf

id	iium-54493
recordtype	eprints
spelling	iium-544932017-12-06T05:05:59Z http://irep.iium.edu.my/54493/ Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus) Khan Chowdhury, Ahmed Jalal Helaluddin, Abul Bashar Mohammed John, Akbar Bulbul, Mahbuba M. N., Nur Hafizah Q Science (General) Introduction: Increasing antibiotic resistance in bacteria is a serious global problem. This study was aimed to extract the antibiotic Chloramphenicol (CAP) from different parts of the fish flesh. Methods: Fish fleshes were excised from two commercially important fishes: Oreochromatis niloticus.(Red Tilapia) and Pangasius hypothalamus (Patin). The fleshes were chosen form head and tail region where generally antibiotics are spiked to avoid bacterial contamination while kept in cold storages of supermarkets. Each fish fleshes were weighed and spiked with 20,40,80 and 160ppm of CAP and stored at 4°C. The CAP residue from the flesh was extracted immediately after the first spiking was considered as Day-1. The whole extraction processes were done for 1,7,14 and 28 days. HPLC (High Performance Liquid Chromatography) was used to determine the presence of CAP residue in the tested fish flesh. Results: The results showed variations on the percentage of antibiotic residue recoveries at different concentration. In this study, CAP did not degrade and showed variations on the presence of antibiotic residue in the fish flesh. The recoveries of the entire CAP spiking samples ranged from 4.0% -100%. It also revealed the presence of antibiotic residue in Malaysian freshwater cultured fishes. Thus, the relevant authorities should take immediate action regarding this issue in order to prevent the harmful effects especially to human. Conclusions: Nevertheless, the study portrays that antibiotic residues can be sustained long time in the food products under storage conditions, which might have harmful effects on human when they consumed these frozen fishes. Kulliyyah of Medicine, International Islamic University Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54493/1/65.pdf Khan Chowdhury, Ahmed Jalal and Helaluddin, Abul Bashar Mohammed and John, Akbar and Bulbul, Mahbuba and M. N., Nur Hafizah (2016) Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus). International Medical Journal of Malaysia, 15 (supplement). p. 110. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-110.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Khan Chowdhury, Ahmed Jalal Helaluddin, Abul Bashar Mohammed John, Akbar Bulbul, Mahbuba M. N., Nur Hafizah Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
description	Introduction: Increasing antibiotic resistance in bacteria is a serious global problem. This study was aimed to extract the antibiotic Chloramphenicol (CAP) from different parts of the fish flesh. Methods: Fish fleshes were excised from two commercially important fishes: Oreochromatis niloticus.(Red Tilapia) and Pangasius hypothalamus (Patin). The fleshes were chosen form head and tail region where generally antibiotics are spiked to avoid bacterial contamination while kept in cold storages of supermarkets. Each fish fleshes were weighed and spiked with 20,40,80 and 160ppm of CAP and stored at 4°C. The CAP residue from the flesh was extracted immediately after the first spiking was considered as Day-1. The whole extraction processes were done for 1,7,14 and 28 days. HPLC (High Performance Liquid Chromatography) was used to determine the presence of CAP residue in the tested fish flesh. Results: The results showed variations on the percentage of antibiotic residue recoveries at different concentration. In this study, CAP did not degrade and showed variations on the presence of antibiotic residue in the fish flesh. The recoveries of the entire CAP spiking samples ranged from 4.0% -100%. It also revealed the presence of antibiotic residue in Malaysian freshwater cultured fishes. Thus, the relevant authorities should take immediate action regarding this issue in order to prevent the harmful effects especially to human. Conclusions: Nevertheless, the study portrays that antibiotic residues can be sustained long time in the food products under storage conditions, which might have harmful effects on human when they consumed these frozen fishes.
format	Article
author	Khan Chowdhury, Ahmed Jalal Helaluddin, Abul Bashar Mohammed John, Akbar Bulbul, Mahbuba M. N., Nur Hafizah
author_facet	Khan Chowdhury, Ahmed Jalal Helaluddin, Abul Bashar Mohammed John, Akbar Bulbul, Mahbuba M. N., Nur Hafizah
author_sort	Khan Chowdhury, Ahmed Jalal
title	Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
title_short	Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
title_full	Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
title_fullStr	Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
title_full_unstemmed	Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)
title_sort	persistence of chloramphenicol in the fish flesh patin (pangasius hypothalamus) and tilapia (oreochromis niloticus)
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/54493/ http://irep.iium.edu.my/54493/ http://irep.iium.edu.my/54493/1/65.pdf
first_indexed	2023-09-18T21:17:06Z
last_indexed	2023-09-18T21:17:06Z
_version_	1777411635779993600

Persistence of chloramphenicol in the fish flesh patin (Pangasius hypothalamus) and tilapia (Oreochromis niloticus)

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