Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins

In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solut...

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Main Authors:	Idris, Zulkarnain Mohamed, Mohamed Dzahir, Mohd Irfan Hatim, Jamal, Parveen, Ahmad Barkat, Anumsima, Xian, R. L.W.
Format:	Conference or Workshop Item
Language:	English English
Published:	IOP Publishing 2017
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf

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recordtype	eprints
spelling	iium-544312018-06-26T03:55:47Z http://irep.iium.edu.my/54431/ Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins Idris, Zulkarnain Mohamed Mohamed Dzahir, Mohd Irfan Hatim Jamal, Parveen Ahmad Barkat, Anumsima Xian, R. L.W. TP Chemical technology TP248.13 Biotechnology In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solution pH and ethanol concentration by static adsorption/ desorption method. From the results, it was found that the adsorption capacity for both resins had has no significant difference. Then, the kinetic adsorption data were analyzed with both pseudo-first-order and pseudo-second-order equations and the later performed better. The adsorption isotherm data were fitted well by both Langmuir and Freundlich models. Meanwhile in desorption study, HP-20 and XAD-7HP gave 90.52% and 88.28% recoveries, respectively. Considering the desorption results of the macroporous resins, HP-20 and XAD-7HP were packed in chromatography column to further purify the phenolics. For dynamic adsorption, breakthrough capacity of HP-20 (0.522) was found to be higher than XAD7HP (0.131). Different ethanol concentrations (30% to 50% (v/v)) were investigated at fixed flowrate (1 ml/min) on phenolics recovery from both types of resins. The highest recovery of bioactive phenolics was 94.3% using XAD-7HP resins at 50% (v/v) of ethanol. Only 77.1% of bioactive phenolics were recovered using HP-20 resin at the same experimental conditions. The purified extract subsequently was analyzed using HPLC. The results showed that three phenolics (gallic acid 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) were identified with higher concentrations as compared to non-purified extract. Finally, the purified extract was tested for scavenging activity against DPPH, and it showed that the activity increased significantly to 90.80% from 59.94% in non-purified extract. IOP Publishing 2017-06-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf application/pdf en http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf Idris, Zulkarnain Mohamed and Mohamed Dzahir, Mohd Irfan Hatim and Jamal, Parveen and Ahmad Barkat, Anumsima and Xian, R. L.W. (2017) Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins. In: 29th Symposium of Malaysian Chemical Engineers (SOMChE 2016), 1st-3rd December 2016, Sarawak, Malaysia. http://iopscience.iop.org/article/10.1088/1757-899X/206/1/012070/meta 10.1088/1757-899X/206/1/012070
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Idris, Zulkarnain Mohamed Mohamed Dzahir, Mohd Irfan Hatim Jamal, Parveen Ahmad Barkat, Anumsima Xian, R. L.W. Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
description	In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solution pH and ethanol concentration by static adsorption/ desorption method. From the results, it was found that the adsorption capacity for both resins had has no significant difference. Then, the kinetic adsorption data were analyzed with both pseudo-first-order and pseudo-second-order equations and the later performed better. The adsorption isotherm data were fitted well by both Langmuir and Freundlich models. Meanwhile in desorption study, HP-20 and XAD-7HP gave 90.52% and 88.28% recoveries, respectively. Considering the desorption results of the macroporous resins, HP-20 and XAD-7HP were packed in chromatography column to further purify the phenolics. For dynamic adsorption, breakthrough capacity of HP-20 (0.522) was found to be higher than XAD7HP (0.131). Different ethanol concentrations (30% to 50% (v/v)) were investigated at fixed flowrate (1 ml/min) on phenolics recovery from both types of resins. The highest recovery of bioactive phenolics was 94.3% using XAD-7HP resins at 50% (v/v) of ethanol. Only 77.1% of bioactive phenolics were recovered using HP-20 resin at the same experimental conditions. The purified extract subsequently was analyzed using HPLC. The results showed that three phenolics (gallic acid 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) were identified with higher concentrations as compared to non-purified extract. Finally, the purified extract was tested for scavenging activity against DPPH, and it showed that the activity increased significantly to 90.80% from 59.94% in non-purified extract.
format	Conference or Workshop Item
author	Idris, Zulkarnain Mohamed Mohamed Dzahir, Mohd Irfan Hatim Jamal, Parveen Ahmad Barkat, Anumsima Xian, R. L.W.
author_facet	Idris, Zulkarnain Mohamed Mohamed Dzahir, Mohd Irfan Hatim Jamal, Parveen Ahmad Barkat, Anumsima Xian, R. L.W.
author_sort	Idris, Zulkarnain Mohamed
title	Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_short	Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_full	Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_fullStr	Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_full_unstemmed	Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_sort	purification of bioactive phenolics from phanerochaete chysosporium biomass extract on selected macroporous resins
publisher	IOP Publishing
publishDate	2017
url	http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/ http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf
first_indexed	2023-09-18T21:17:02Z
last_indexed	2023-09-18T21:17:02Z
_version_	1777411630976466944

Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins

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