Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma

Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent k...

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Main Authors:	Mohd. Ridah, Lailatul Jalilah, Ismail, Aziah, A. Talib, Norlelawati, Muhammad, Siti Aesah @ Naznin, Hussain, Faezahtul Arbaiyah, Zainuddin, Norafiza
Format:	Conference or Workshop Item
Language:	English English
Published:	IIUM 2016
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf

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spelling	iium-543782017-01-20T04:24:58Z http://irep.iium.edu.my/54378/ Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza RC0254 Neoplasms. Tumors. Oncology (including Cancer) Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O6-guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation. IIUM 2016-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf application/pdf en http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf Mohd. Ridah, Lailatul Jalilah and Ismail, Aziah and A. Talib, Norlelawati and Muhammad, Siti Aesah @ Naznin and Hussain, Faezahtul Arbaiyah and Zainuddin, Norafiza (2016) Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
description	Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O6-guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation.
format	Conference or Workshop Item
author	Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza
author_facet	Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza
author_sort	Mohd. Ridah, Lailatul Jalilah
title	Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
title_short	Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
title_full	Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
title_fullStr	Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
title_full_unstemmed	Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
title_sort	epigenetic methylation status of p16, mgmt and spock2 in diffuse large b cell lymphoma
publisher	IIUM
publishDate	2016
url	http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf
first_indexed	2023-09-18T21:16:56Z
last_indexed	2023-09-18T21:16:56Z
_version_	1777411624829714432

Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma

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