Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)

Amoebocyte cells degranulate in response to the bacterial pyrogen, which is the basis for the detection of endotoxin in various medical applications. It is presumed that the captive breeding might impart the cellular reaction by altering their functionality. Crabs under 4 month captivity and wild cr...

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Main Authors:	Sheikh Mohamed, Hassan Ibrahim, John, Akbar, Yunus, Kamaruzzaman, S., Solahuddin
Format:	Conference or Workshop Item
Language:	English
Published:	Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/54269/ http://irep.iium.edu.my/54269/ http://irep.iium.edu.my/54269/1/54269_EFFECT%20OF%20CAPTIVE%20BREEDING.pdf

id	iium-54269
recordtype	eprints
spelling	iium-542692017-02-03T02:40:49Z http://irep.iium.edu.my/54269/ Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas) Sheikh Mohamed, Hassan Ibrahim John, Akbar Yunus, Kamaruzzaman S., Solahuddin QD Chemistry Amoebocyte cells degranulate in response to the bacterial pyrogen, which is the basis for the detection of endotoxin in various medical applications. It is presumed that the captive breeding might impart the cellular reaction by altering their functionality. Crabs under 4 month captivity and wild crabs were bled in triplicate to determine cell viability and density. A total of 3 anticoagulants formulations (A, B and C) were used to obtain the amoebocyte cells. Results showed that the significant reduction in the cell viability of captive crabs compared to the wild crabs (P<0.01). The effect of different anticoagulants on cell viability was also apparent in this study. Cell viability of captive crabs in anticoagulant A, B and C were 40%, 58% and 52% respectively. Whilst, the cell viability of wild crabs was 80%, 86% and 84% respectively. On the contrary, mean cell density in captive crabs were higher compared to the wild crabs (P<0.05) with the mean value of 8×106 cells/ml and 6×106 cells/ml in captive and wild crabs respectively. The performance of different anticoagulants used in this study was in the order of B>C>A (refer methodology). We conclude that the captive breeding has profound negative effect on the amoebocyte viability. Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54269/1/54269_EFFECT%20OF%20CAPTIVE%20BREEDING.pdf Sheikh Mohamed, Hassan Ibrahim and John, Akbar and Yunus, Kamaruzzaman and S., Solahuddin (2016) Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas). In: The 14th Symposium of Malaysian Society of Applied Biology (MSAB 2016), 29th–31st May 2016, Melaka. http://msabsimposium.blogspot.my/p/poster-presentation.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Sheikh Mohamed, Hassan Ibrahim John, Akbar Yunus, Kamaruzzaman S., Solahuddin Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
description	Amoebocyte cells degranulate in response to the bacterial pyrogen, which is the basis for the detection of endotoxin in various medical applications. It is presumed that the captive breeding might impart the cellular reaction by altering their functionality. Crabs under 4 month captivity and wild crabs were bled in triplicate to determine cell viability and density. A total of 3 anticoagulants formulations (A, B and C) were used to obtain the amoebocyte cells. Results showed that the significant reduction in the cell viability of captive crabs compared to the wild crabs (P<0.01). The effect of different anticoagulants on cell viability was also apparent in this study. Cell viability of captive crabs in anticoagulant A, B and C were 40%, 58% and 52% respectively. Whilst, the cell viability of wild crabs was 80%, 86% and 84% respectively. On the contrary, mean cell density in captive crabs were higher compared to the wild crabs (P<0.05) with the mean value of 8×106 cells/ml and 6×106 cells/ml in captive and wild crabs respectively. The performance of different anticoagulants used in this study was in the order of B>C>A (refer methodology). We conclude that the captive breeding has profound negative effect on the amoebocyte viability.
format	Conference or Workshop Item
author	Sheikh Mohamed, Hassan Ibrahim John, Akbar Yunus, Kamaruzzaman S., Solahuddin
author_facet	Sheikh Mohamed, Hassan Ibrahim John, Akbar Yunus, Kamaruzzaman S., Solahuddin
author_sort	Sheikh Mohamed, Hassan Ibrahim
title	Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
title_short	Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
title_full	Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
title_fullStr	Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
title_full_unstemmed	Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)
title_sort	effect of captive breeding on the amoebocyte cell viability in malaysian horseshoe crab (tachypleus gigas)
publisher	Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia
publishDate	2016
url	http://irep.iium.edu.my/54269/ http://irep.iium.edu.my/54269/ http://irep.iium.edu.my/54269/1/54269_EFFECT%20OF%20CAPTIVE%20BREEDING.pdf
first_indexed	2023-09-18T21:16:47Z
last_indexed	2023-09-18T21:16:47Z
_version_	1777411615957712896

Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)

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