Effects of ionizing radiation on chondrocytes growth: work in progress
Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/53947/ http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf |
| Summary: | Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes.
Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches
80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a
typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage.
Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control.
Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes. |
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