Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.

This study attempted to examine the feasibility of using medical Computerized Tomography (CT) imaging scanner to monitor the growth of tissue engineered constructs (TECs) in vitro. A preliminary CT examination was performed on TECs prepared from serially expanded chondrocytes seeded on poly (lactic-...

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Main Authors:	Mat Nawi, Nur Farhana, Zainuddin, Zainul Ibrahim, Md Nazir, Noorhidayah, Mohamad, Mohd Yusof, Ahmad Radzi, Muhammad Aa'zamuddin, Hashim, Rosyafirah, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English English
Published:	International Islamic University Malaysia (IIUM) 2016
Subjects:	RZ Other systems of medicine
Online Access:	http://irep.iium.edu.my/53939/ http://irep.iium.edu.my/53939/1/NurFarhana.ICBIOE2016.pdf http://irep.iium.edu.my/53939/2/NurFarhana.ICBIOE2016_Oral.pdf

id	iium-53939
recordtype	eprints
spelling	iium-539392016-12-26T04:32:33Z http://irep.iium.edu.my/53939/ Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds. Mat Nawi, Nur Farhana Zainuddin, Zainul Ibrahim Md Nazir, Noorhidayah Mohamad, Mohd Yusof Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Sha'ban, Munirah RZ Other systems of medicine This study attempted to examine the feasibility of using medical Computerized Tomography (CT) imaging scanner to monitor the growth of tissue engineered constructs (TECs) in vitro. A preliminary CT examination was performed on TECs prepared from serially expanded chondrocytes seeded on poly (lactic-co-glycolic acid) (PLGA) based scaffolds that acted as cell carriers. One day old PLGA with fibrin and cells (PFC), PLGA and cells (PC) as well as PLGA with Fibrin (PF) constructs were stored in normal saline prior to CT examination. The kVp used was 80 Kvpwhile the effective mAs was 11 mAs. Sharp kernel was chosen as the reconstruction parameters and slice thickness used was 0.1mm.A PLGA without cells or fibrin was used as control. X-ray attenuation of TECs, measured in Hounsfield units (HU) or, also known as CT values were observed. The results indicated that the control PLGA, the PFC, the PC and also the PF constructs gave CT values between -665HU to 477HU, between 149HU to 181HU, between -120HU to 263HU and -209HU to 185HU respectively. These initial results indicate that the x-ray absorption-based quantitative differentiation between TECs could be appreciated. The CT values documented suggest that the differences in attenuation values are dependent on the condition of the scaffolds or the TECs themselves. Furthermore, the use of intravascular ultrasound (IVUS) software installed in the CT system was found to be useful in appreciating the different compositions within the scanned samples. It is hoped that the outcomes of this preliminary study can serve as a baseline for future research to look for an alternative method to quantitatively monitor the growth of TECs in vitro as well as in vivo. International Islamic University Malaysia (IIUM) 2016-07-25 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/53939/1/NurFarhana.ICBIOE2016.pdf application/pdf en http://irep.iium.edu.my/53939/2/NurFarhana.ICBIOE2016_Oral.pdf Mat Nawi, Nur Farhana and Zainuddin, Zainul Ibrahim and Md Nazir, Noorhidayah and Mohamad, Mohd Yusof and Ahmad Radzi, Muhammad Aa'zamuddin and Hashim, Rosyafirah and Sha'ban, Munirah (2016) Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 2-27 Jul 2016, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Mat Nawi, Nur Farhana Zainuddin, Zainul Ibrahim Md Nazir, Noorhidayah Mohamad, Mohd Yusof Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Sha'ban, Munirah Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
description	This study attempted to examine the feasibility of using medical Computerized Tomography (CT) imaging scanner to monitor the growth of tissue engineered constructs (TECs) in vitro. A preliminary CT examination was performed on TECs prepared from serially expanded chondrocytes seeded on poly (lactic-co-glycolic acid) (PLGA) based scaffolds that acted as cell carriers. One day old PLGA with fibrin and cells (PFC), PLGA and cells (PC) as well as PLGA with Fibrin (PF) constructs were stored in normal saline prior to CT examination. The kVp used was 80 Kvpwhile the effective mAs was 11 mAs. Sharp kernel was chosen as the reconstruction parameters and slice thickness used was 0.1mm.A PLGA without cells or fibrin was used as control. X-ray attenuation of TECs, measured in Hounsfield units (HU) or, also known as CT values were observed. The results indicated that the control PLGA, the PFC, the PC and also the PF constructs gave CT values between -665HU to 477HU, between 149HU to 181HU, between -120HU to 263HU and -209HU to 185HU respectively. These initial results indicate that the x-ray absorption-based quantitative differentiation between TECs could be appreciated. The CT values documented suggest that the differences in attenuation values are dependent on the condition of the scaffolds or the TECs themselves. Furthermore, the use of intravascular ultrasound (IVUS) software installed in the CT system was found to be useful in appreciating the different compositions within the scanned samples. It is hoped that the outcomes of this preliminary study can serve as a baseline for future research to look for an alternative method to quantitatively monitor the growth of TECs in vitro as well as in vivo.
format	Conference or Workshop Item
author	Mat Nawi, Nur Farhana Zainuddin, Zainul Ibrahim Md Nazir, Noorhidayah Mohamad, Mohd Yusof Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Sha'ban, Munirah
author_facet	Mat Nawi, Nur Farhana Zainuddin, Zainul Ibrahim Md Nazir, Noorhidayah Mohamad, Mohd Yusof Ahmad Radzi, Muhammad Aa'zamuddin Hashim, Rosyafirah Sha'ban, Munirah
author_sort	Mat Nawi, Nur Farhana
title	Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
title_short	Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
title_full	Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
title_fullStr	Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
title_full_unstemmed	Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
title_sort	exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.
publisher	International Islamic University Malaysia (IIUM)
publishDate	2016
url	http://irep.iium.edu.my/53939/ http://irep.iium.edu.my/53939/1/NurFarhana.ICBIOE2016.pdf http://irep.iium.edu.my/53939/2/NurFarhana.ICBIOE2016_Oral.pdf
first_indexed	2023-09-18T21:16:18Z
last_indexed	2023-09-18T21:16:18Z
_version_	1777411585501822976

Exploring the feasibility in using medical computerized tomography to monitorgrowth progression in tissue engineered construct prepared from cells seeded on poly(lactic-co-glycolic acid) based scaffolds.

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