Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study

Objective To assess the immunopotentiality of Ayurvedic polyherbal preparations, “Saribadi” and “Anantamul Salsa”. Methods Freshly prepared BALB/c mice splenocytes were cultured with “Saribadi” or “Anantamul Salsa” treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%, 3.00% and 4.00% (v/v)]...

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Main Authors:	Sarker, Md Moklesur Rahman, Ming, Long Chiau, Sarker, Md Zaidul Islam, Choudhuri, Mohammad Shahabuddin Kabir
Format:	Article
Language:	English English
Published:	Elsevier BV 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/1/53915_Immunopotentiality%20of%20Ayurvedi.pdf http://irep.iium.edu.my/53915/2/53915_Immunopotentiality%20of%20Ayurvedi_SCOPUS.pdf

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spelling	iium-539152017-04-13T08:21:18Z http://irep.iium.edu.my/53915/ Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study Sarker, Md Moklesur Rahman Ming, Long Chiau Sarker, Md Zaidul Islam Choudhuri, Mohammad Shahabuddin Kabir RS Pharmacy and materia medica Objective To assess the immunopotentiality of Ayurvedic polyherbal preparations, “Saribadi” and “Anantamul Salsa”. Methods Freshly prepared BALB/c mice splenocytes were cultured with “Saribadi” or “Anantamul Salsa” treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%, 3.00% and 4.00% (v/v)] at 37 °C for 5 days. The immunoglobulin M (IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, respectively. Endotoxin contamination was assessed by treating the preparations with polymyxin B. Results The doses of “Saribadi” [0.25%, 0.50%, 0.75% and 1.00% (v/v)] significantly increased IgM productions (0.966, 0.728, 0.695 and 0.615 μg/mL vs. control 0.265 μg/mL) and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density (OD) vs. control 0.162 OD]. Similarly, the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00% and 1.50% (v/v)] promoted IgM productions (0.933, 0.919, 0.917 and 0.892 μg/mL vs. control 0.502 μg/mL) and the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00%, 1.50%, 2.00%, and 3.00% (v/v)] stimulated lymphocytes proliferation (absorbance 0.395, 0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of “Saribadi” and “Anantamul Salsa” was not retarded by the treatment of preparations with polymyxin B. Conclusions Immunomodulatory activity of “Saribadi” and “Anantamul Salsa” was unveiled for the first time. “Saribadi” and “Anantamul Salsa” possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM production. These preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required Elsevier BV 2016-07-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53915/1/53915_Immunopotentiality%20of%20Ayurvedi.pdf application/pdf en http://irep.iium.edu.my/53915/2/53915_Immunopotentiality%20of%20Ayurvedi_SCOPUS.pdf Sarker, Md Moklesur Rahman and Ming, Long Chiau and Sarker, Md Zaidul Islam and Choudhuri, Mohammad Shahabuddin Kabir (2016) Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study. Asian Pacific Journal of Tropical Biomedicine, 6 (7). pp. 568-573. ISSN 2221-1691 http://ac.els-cdn.com/S2221169116300867/1-s2.0-S2221169116300867-main.pdf?_tid=c371ce86-201c-11e7-b2e6-00000aab0f6b&acdnat=1492069536_3f521b9e744905c654abbb79850a6165 10.1016/j.apjtb.2016.05.004
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Sarker, Md Moklesur Rahman Ming, Long Chiau Sarker, Md Zaidul Islam Choudhuri, Mohammad Shahabuddin Kabir Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
description	Objective To assess the immunopotentiality of Ayurvedic polyherbal preparations, “Saribadi” and “Anantamul Salsa”. Methods Freshly prepared BALB/c mice splenocytes were cultured with “Saribadi” or “Anantamul Salsa” treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%, 3.00% and 4.00% (v/v)] at 37 °C for 5 days. The immunoglobulin M (IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, respectively. Endotoxin contamination was assessed by treating the preparations with polymyxin B. Results The doses of “Saribadi” [0.25%, 0.50%, 0.75% and 1.00% (v/v)] significantly increased IgM productions (0.966, 0.728, 0.695 and 0.615 μg/mL vs. control 0.265 μg/mL) and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density (OD) vs. control 0.162 OD]. Similarly, the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00% and 1.50% (v/v)] promoted IgM productions (0.933, 0.919, 0.917 and 0.892 μg/mL vs. control 0.502 μg/mL) and the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00%, 1.50%, 2.00%, and 3.00% (v/v)] stimulated lymphocytes proliferation (absorbance 0.395, 0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of “Saribadi” and “Anantamul Salsa” was not retarded by the treatment of preparations with polymyxin B. Conclusions Immunomodulatory activity of “Saribadi” and “Anantamul Salsa” was unveiled for the first time. “Saribadi” and “Anantamul Salsa” possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM production. These preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required
format	Article
author	Sarker, Md Moklesur Rahman Ming, Long Chiau Sarker, Md Zaidul Islam Choudhuri, Mohammad Shahabuddin Kabir
author_facet	Sarker, Md Moklesur Rahman Ming, Long Chiau Sarker, Md Zaidul Islam Choudhuri, Mohammad Shahabuddin Kabir
author_sort	Sarker, Md Moklesur Rahman
title	Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
title_short	Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
title_full	Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
title_fullStr	Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
title_full_unstemmed	Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study
title_sort	immunopotentiality of ayurvedic polyherbal formulations “saribadi” and “anantamul salsa” with augmentation of igm production and lymphocytes proliferation: a preliminary study
publisher	Elsevier BV
publishDate	2016
url	http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/ http://irep.iium.edu.my/53915/1/53915_Immunopotentiality%20of%20Ayurvedi.pdf http://irep.iium.edu.my/53915/2/53915_Immunopotentiality%20of%20Ayurvedi_SCOPUS.pdf
first_indexed	2023-09-18T21:16:16Z
last_indexed	2023-09-18T21:16:16Z
_version_	1777411583015649280

Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study

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